##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR580203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6421295 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5231964891817 34.0 31.0 34.0 31.0 34.0 2 32.65010578084327 34.0 31.0 34.0 31.0 34.0 3 32.70145508032258 34.0 31.0 34.0 31.0 34.0 4 36.21678664506147 37.0 35.0 37.0 35.0 37.0 5 36.160674599126814 37.0 35.0 37.0 35.0 37.0 6 36.19614610448515 37.0 37.0 37.0 35.0 37.0 7 36.13957293661169 37.0 36.0 37.0 35.0 37.0 8 36.13564273250178 37.0 36.0 37.0 35.0 37.0 9 37.85214991680027 39.0 38.0 39.0 35.0 39.0 10-11 37.80565851592242 39.0 38.0 39.0 35.0 39.0 12-13 37.76582262612136 39.0 38.0 39.0 35.0 39.0 14-15 39.15312098260553 41.0 39.0 41.0 36.0 41.0 16-17 39.14598877640725 40.0 39.0 41.0 36.0 41.0 18-19 39.132459184634875 40.0 38.5 41.0 36.0 41.0 20-21 39.1006070426604 40.0 39.0 41.0 35.0 41.0 22-23 39.017861506129215 40.0 38.5 41.0 35.0 41.0 24-25 38.944808173429195 40.0 38.0 41.0 35.0 41.0 26-27 38.81899663541388 40.0 38.0 41.0 35.0 41.0 28-29 38.7183464550375 40.0 38.0 41.0 35.0 41.0 30-31 38.56898304781201 40.0 38.0 41.0 34.0 41.0 32-33 38.46213045810853 40.0 38.0 41.0 34.0 41.0 34-35 38.36804647660635 40.0 38.0 41.0 34.0 41.0 36-37 38.24104179920094 40.0 38.0 41.0 34.0 41.0 38-39 38.114900888372205 40.0 38.0 41.0 33.0 41.0 40-41 37.996272402996595 40.0 37.0 41.0 33.0 41.0 42-43 37.99091787559986 40.0 37.0 41.0 33.0 41.0 44-45 37.940233784618215 40.0 37.0 41.0 33.0 41.0 46-47 37.746553148547136 40.0 37.0 41.0 33.0 41.0 48-49 37.532920150841846 40.0 36.5 41.0 33.0 41.0 50-51 37.32635652154277 40.0 36.0 41.0 32.0 41.0 52-53 37.14141010497104 39.0 35.5 41.0 32.0 41.0 54-55 36.908973657182855 39.0 35.0 41.0 31.0 41.0 56-57 36.64610619820456 39.0 35.0 41.0 31.0 41.0 58-59 36.38098802500119 38.0 35.0 40.0 31.0 41.0 60-61 36.11637784901644 38.0 35.0 40.0 31.0 41.0 62-63 35.80319514988799 37.0 35.0 40.0 30.0 41.0 64-65 35.49448810559241 36.5 35.0 40.0 30.0 41.0 66-67 35.17254556907913 36.0 34.0 39.0 29.0 41.0 68-69 34.8586823218681 35.5 34.0 39.0 29.0 41.0 70-71 34.55422831375914 35.0 34.0 38.5 29.0 40.5 72-73 34.25880114525185 35.0 34.0 37.5 29.0 40.0 74-75 33.92798430846114 35.0 34.0 37.0 28.5 39.5 76-77 32.42203472975467 34.0 31.5 35.5 25.5 38.5 78-79 33.320740520409046 35.0 33.0 36.0 27.5 39.0 80-81 33.320871179411625 35.0 33.0 36.0 28.0 37.5 82-83 33.116226244083165 35.0 33.0 35.5 28.0 37.0 84-85 32.924084004861946 35.0 33.0 35.0 27.0 37.0 86-87 32.7384236357308 35.0 33.0 35.0 27.0 36.0 88-89 32.5740338202808 35.0 33.0 35.0 27.0 36.0 90-91 32.41530571325566 35.0 33.0 35.0 26.5 36.0 92-93 32.26152543996187 35.0 33.0 35.0 26.0 35.5 94-95 32.1368733253962 35.0 33.0 35.0 25.0 35.0 96-97 32.020768552137845 35.0 32.0 35.0 25.0 35.0 98-99 31.920947098677136 35.0 32.0 35.0 25.0 35.0 100 30.044343236060637 33.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07527169001370737 1101 2 -0.07439556145646264 1101 3 -0.04435957254339229 1101 4 6.824226843917813E-4 1101 5 -0.0468962093578682 1101 6 -0.04548861516574476 1101 7 -0.056564347189734576 1101 8 -0.04008076200510402 1101 9 -0.12096953844508107 1101 10-11 -0.0325807414783128 1101 12-13 -0.07598129765730022 1101 14-15 -0.08602229363624048 1101 16-17 -0.08614485650088 1101 18-19 -0.08378776861517423 1101 20-21 -0.09611289261532363 1101 22-23 -0.07648858660052582 1101 24-25 -0.12520410430386875 1101 26-27 -0.15750694004845656 1101 28-29 -0.1245673122953832 1101 30-31 -0.1355340172174877 1101 32-33 -0.12273180606306511 1101 34-35 -0.16320465274529283 1101 36-37 -0.10980935108159429 1101 38-39 -0.19320388904370844 1101 40-41 -0.158202987273107 1101 42-43 -0.12209937330380427 1101 44-45 -0.09462053120833502 1101 46-47 -0.18548834781031331 1101 48-49 -0.12634986044312058 1101 50-51 -0.11044833197068016 1101 52-53 -0.10274314486332514 1101 54-55 -0.14654417553486354 1101 56-57 -0.15759816129101267 1101 58-59 -0.11832883355761226 1101 60-61 -0.10043187637816686 1101 62-63 -0.07562179308526851 1101 64-65 -0.14348935170831112 1101 66-67 -0.20073158502722066 1101 68-69 -0.16981403267735118 1101 70-71 -0.18542805496512926 1101 72-73 -0.17332325525930514 1101 74-75 -0.21694255299865262 1101 76-77 -0.26534868711047466 1101 78-79 -0.22086102889333858 1101 80-81 -0.17074068845393953 1101 82-83 -0.15050575836543345 1101 84-85 -0.12859144556818336 1101 86-87 -0.2146247809635753 1101 88-89 -0.17003313174756585 1101 90-91 -0.1827125159420362 1101 92-93 -0.21055039272116716 1101 94-95 -0.22101062462740373 1101 96-97 -0.23412488774504325 1101 98-99 -0.3135254373138743 1101 100 -0.46864838535617537 1102 1 -0.015961249645521036 1102 2 -0.0084035800539084 1102 3 -0.013989733879817834 1102 4 0.016310241220217847 1102 5 -0.003817174325163819 1102 6 0.010876606871313754 1102 7 -0.00825218070125544 1102 8 0.017662024442898883 1102 9 -0.01669585631425008 1102 10-11 0.06691247006992285 1102 12-13 -0.007829210852094093 1102 14-15 -0.021683731477502022 1102 16-17 -0.012458180945444042 1102 18-19 -0.008672177592870867 1102 20-21 -0.00434867258145033 1102 22-23 -0.05570518927078183 1102 24-25 -0.016297034225026152 1102 26-27 -0.019246560091566778 1102 28-29 -0.028865369372503835 1102 30-31 -0.08213745320812649 1102 32-33 -0.03701131596881879 1102 34-35 -0.08454447982930446 1102 36-37 -0.10034476986596985 1102 38-39 -0.10935587121785062 1102 40-41 -0.1213309855522482 1102 42-43 -0.028030946341260687 1102 44-45 -0.08156047005468992 1102 46-47 -0.04941199519077344 1102 48-49 -0.014173054987338674 1102 50-51 -0.13139108708781322 1102 52-53 -0.04711992658713626 1102 54-55 -0.08241271273997341 1102 56-57 -0.0174611296684688 1102 58-59 -0.030401285601222128 1102 60-61 -0.058070090214840775 1102 62-63 -0.055388118015578414 1102 64-65 -0.10642869257936383 1102 66-67 -0.01998986768658284 1102 68-69 -0.040066573487855806 1102 70-71 -0.04169924056368046 1102 72-73 -0.15387101057225294 1102 74-75 -0.1370866632926564 1102 76-77 -0.12340463608792618 1102 78-79 -0.07708904687116558 1102 80-81 -0.1183383679824388 1102 82-83 -0.11683567180222099 1102 84-85 -0.19954885780229858 1102 86-87 -0.09157088953554648 1102 88-89 -0.11809392064241564 1102 90-91 -0.14831329991512376 1102 92-93 -0.1633630101753809 1102 94-95 -0.1052325523758526 1102 96-97 -0.21447833031164976 1102 98-99 -0.21881827862011605 1102 100 -0.12279575031926626 1103 1 0.0047972677305310185 1103 2 -0.0028130563650918816 1103 3 -0.010912656467269244 1103 4 0.017415450310728886 1103 5 0.02165167296313797 1103 6 0.03340845788707725 1103 7 0.05147105886916137 1103 8 0.025275556297835067 1103 9 -0.0521819202163627 1103 10-11 0.03496651633845005 1103 12-13 0.01320641442426762 1103 14-15 -0.0492634493873183 1103 16-17 -0.04776570374260558 1103 18-19 -0.054429405964633304 1103 20-21 -0.043551468263629545 1103 22-23 -0.021164315785433985 1103 24-25 -0.035029306913322955 1103 26-27 0.005680555122445696 1103 28-29 -0.04033834819976079 1103 30-31 -0.07357398123583891 1103 32-33 -0.047590902736459384 1103 34-35 -0.028498237080654576 1103 36-37 -0.07129686206004493 1103 38-39 -0.11220831161121225 1103 40-41 -0.06565315984468612 1103 42-43 -0.08039858101277986 1103 44-45 -0.05714403872470797 1103 46-47 -0.0421849684697122 1103 48-49 -0.045140066004961454 1103 50-51 -0.04153616732272525 1103 52-53 -0.09953095306608617 1103 54-55 -0.10440913659372342 1103 56-57 0.0069813899874517915 1103 58-59 -0.024112976253640284 1103 60-61 -0.026051223675544577 1103 62-63 -0.008952172841972583 1103 64-65 0.0017027808141847345 1103 66-67 -0.05673940798842381 1103 68-69 -0.07847500626600379 1103 70-71 -0.09686314586558353 1103 72-73 -0.11063103944594843 1103 74-75 -0.09878535797965782 1103 76-77 -0.06642232402895587 1103 78-79 -0.05728387426509585 1103 80-81 -0.09572291382979614 1103 82-83 -0.040978063622539196 1103 84-85 -0.01263114447348812 1103 86-87 -0.02594113808359566 1103 88-89 -3.076229821701304E-4 1103 90-91 -0.038092644798311426 1103 92-93 -0.05070084082600346 1103 94-95 -0.00300151012621086 1103 96-97 -0.087604496370151 1103 98-99 -0.11571970846213375 1103 100 0.057762898535230534 1104 1 -0.03505891409459849 1104 2 -0.06861154974289718 1104 3 -0.039764339578688634 1104 4 0.0018856502785737916 1104 5 -0.004042212300149117 1104 6 0.012503965074323276 1104 7 0.021466864261199703 1104 8 0.009712348590944941 1104 9 0.02598902824058058 1104 10-11 0.015291367345952267 1104 12-13 -0.018367221592185956 1104 14-15 -0.04078432045913161 1104 16-17 -0.005241436889875217 1104 18-19 -0.01889336397220376 1104 20-21 -0.021920225320961606 1104 22-23 -0.049949503238053694 1104 24-25 -0.054848702999002796 1104 26-27 -0.034788034137257284 1104 28-29 0.011378011051021986 1104 30-31 -0.02144348936644036 1104 32-33 -0.06130355437473867 1104 34-35 -7.487408265660633E-4 1104 36-37 -0.04812813345517952 1104 38-39 0.0455701153162309 1104 40-41 -0.0027984314132467603 1104 42-43 -0.0361427895832378 1104 44-45 0.043250725967631354 1104 46-47 0.04795129568109502 1104 48-49 0.021606829698754382 1104 50-51 0.040526721958947576 1104 52-53 -0.037869177000608545 1104 54-55 -0.03823438062093487 1104 56-57 -0.18644130754375254 1104 58-59 -0.09011311451985904 1104 60-61 -0.03924565674211067 1104 62-63 -0.02504925064282304 1104 64-65 -0.021906694657346293 1104 66-67 -0.027007949481010485 1104 68-69 -0.06803847798410345 1104 70-71 -0.06382865670396143 1104 72-73 -0.10624205955623012 1104 74-75 -0.08038857204306282 1104 76-77 -0.06614451283905964 1104 78-79 -0.03525256509317387 1104 80-81 0.015576046629398377 1104 82-83 0.04603375707309709 1104 84-85 0.009675098672310867 1104 86-87 0.029365520526866362 1104 88-89 0.034678497780710416 1104 90-91 -0.013639838666854587 1104 92-93 -0.01467620493036037 1104 94-95 -0.04541957307517919 1104 96-97 -0.01527229833829935 1104 98-99 -0.068272431780386 1104 100 0.12039538867110977 1105 1 0.07325296551955063 1105 2 0.06626116696288875 1105 3 0.07964389539832695 1105 4 0.0694349989932519 1105 5 0.03347177474034879 1105 6 0.019501678277379142 1105 7 0.02629603465184971 1105 8 0.06876086911388057 1105 9 0.023257236602546527 1105 10-11 0.03794326429647299 1105 12-13 0.06995085997751715 1105 14-15 0.08481593599983484 1105 16-17 0.04385450088084042 1105 18-19 0.04523435585340252 1105 20-21 0.021280216484505843 1105 22-23 0.02192862260714179 1105 24-25 0.038398792751152655 1105 26-27 -0.027453426972414263 1105 28-29 -7.881016558215492E-4 1105 30-31 0.014113421522999658 1105 32-33 0.0239814623292105 1105 34-35 0.048306686583799774 1105 36-37 -0.011051142250977364 1105 38-39 -0.01970119650057711 1105 40-41 0.003637748848873912 1105 42-43 0.0205921894202703 1105 44-45 0.04611075740883308 1105 46-47 0.022545726337398264 1105 48-49 0.05963878169072245 1105 50-51 0.04674308836127494 1105 52-53 0.0386914949532553 1105 54-55 0.1051574584456958 1105 56-57 0.04411804145011189 1105 58-59 -0.00949787154981152 1105 60-61 -0.01708403836301642 1105 62-63 0.002098109379559787 1105 64-65 0.06749458750329751 1105 66-67 -0.003217556587792103 1105 68-69 -0.013059881129841244 1105 70-71 0.03276762669496236 1105 72-73 0.039361219440074535 1105 74-75 0.07796556219918926 1105 76-77 -7.359044442480922E-4 1105 78-79 0.09618378933769378 1105 80-81 0.04323076666177883 1105 82-83 0.18974949840313826 1105 84-85 0.15879070331988743 1105 86-87 0.0651706412325126 1105 88-89 0.09626210246894829 1105 90-91 0.10608494478204733 1105 92-93 0.08844029837634793 1105 94-95 0.1340501865600885 1105 96-97 0.11321483735164506 1105 98-99 0.1020438442141689 1105 100 0.22239725070273053 1106 1 0.016788526448912933 1106 2 -0.03329605143176195 1106 3 -0.0349056425047678 1106 4 -0.003088612814934777 1106 5 -0.024178552504352524 1106 6 -0.017350384809112995 1106 7 -0.051688888264408206 1106 8 -0.04148886092824711 1106 9 0.08988157184712975 1106 10-11 0.05065541569594245 1106 12-13 -0.01389057716158959 1106 14-15 -0.041125748030317766 1106 16-17 -0.04227295979149659 1106 18-19 0.041334998175692306 1106 20-21 0.030011389140945255 1106 22-23 -0.07265510995966196 1106 24-25 0.00353171848612277 1106 26-27 -0.018403704027768697 1106 28-29 0.018938467775321044 1106 30-31 -0.057764496683567756 1106 32-33 -0.00201984056605653 1106 34-35 0.020598388104176024 1106 36-37 0.024805219882935603 1106 38-39 0.08135199407193738 1106 40-41 0.10124630233094933 1106 42-43 0.0710380381526079 1106 44-45 0.1070829859680984 1106 46-47 0.09555957150071492 1106 48-49 0.09714895897573683 1106 50-51 0.02669411572207281 1106 52-53 0.11970843766135175 1106 54-55 0.0400328215580501 1106 56-57 0.015101752724461903 1106 58-59 -0.010779119490273104 1106 60-61 0.02556656771141519 1106 62-63 0.12103716227102268 1106 64-65 0.04917337058898141 1106 66-67 0.08235989977534786 1106 68-69 0.08148344647480599 1106 70-71 0.04825187788506469 1106 72-73 0.08549466227744063 1106 74-75 0.06848750609864851 1106 76-77 0.054668373310711615 1106 78-79 0.14961598048307678 1106 80-81 0.19752385726116017 1106 82-83 0.08042976689082337 1106 84-85 0.1464626018461459 1106 86-87 0.13336420482850997 1106 88-89 0.12441247280552403 1106 90-91 0.10814651038560896 1106 92-93 0.13349497435086022 1106 94-95 0.1327000169809196 1106 96-97 0.11860266415275689 1106 98-99 0.14601932240715243 1106 100 0.2064068221667874 1107 1 0.006968113097819639 1107 2 -0.021886226307294976 1107 3 0.01756624417333086 1107 4 0.020684105423697474 1107 5 0.021382572960931157 1107 6 -0.020148049863692563 1107 7 -0.02538826526877358 1107 8 0.03795942356409654 1107 9 -0.016040321824029036 1107 10-11 -0.055478330841268075 1107 12-13 -0.0016502097929205206 1107 14-15 -0.035010809139841115 1107 16-17 -0.03331239818755449 1107 18-19 -0.03008905843618237 1107 20-21 -0.0033724905097400892 1107 22-23 0.0022186469321852087 1107 24-25 0.0027768730327153435 1107 26-27 -0.07612383201300332 1107 28-29 -0.0028427623650131295 1107 30-31 0.004155024255119599 1107 32-33 -0.023511435400514813 1107 34-35 -0.04757729470050265 1107 36-37 -0.056170041866025144 1107 38-39 -0.05244657394598562 1107 40-41 -0.08202254764218253 1107 42-43 -0.11251049735373186 1107 44-45 -0.06914587843281339 1107 46-47 -0.13614082133741334 1107 48-49 -0.09755006960256196 1107 50-51 -0.10439621251001086 1107 52-53 -0.11085494521228156 1107 54-55 -0.05631319497501863 1107 56-57 -0.09795281521494559 1107 58-59 -0.14953975323312818 1107 60-61 -0.05735034739285538 1107 62-63 -0.047511287355646914 1107 64-65 0.011152197570901023 1107 66-67 0.015806618804077743 1107 68-69 -0.06857788387482344 1107 70-71 -0.04824419496053167 1107 72-73 -0.08007836365576537 1107 74-75 -0.015082793768605995 1107 76-77 -0.06661601842815656 1107 78-79 0.010271708534077106 1107 80-81 0.008250656869549289 1107 82-83 0.014206374251436671 1107 84-85 -0.008704789890266795 1107 86-87 0.012338617595617052 1107 88-89 -0.04646353658726099 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-0.15595597143475715 2316 44-45 -0.1926587704533418 2316 46-47 -0.2008376167465329 2316 48-49 -0.19784784072857775 2316 50-51 -0.18509016181117488 2316 52-53 -0.12863902655506365 2316 54-55 -0.17538518889475085 2316 56-57 -0.3636360010736368 2316 58-59 -0.3799987206252453 2316 60-61 -0.19205890303630468 2316 62-63 -0.19147848424505298 2316 64-65 -0.16820559851013428 2316 66-67 -0.24820975285734193 2316 68-69 -0.17111141862821455 2316 70-71 -0.12187019536565913 2316 72-73 -0.1145770739280394 2316 74-75 -0.17335855742496875 2316 76-77 -0.14642678139654208 2316 78-79 -0.1300751684456145 2316 80-81 -0.18579172795347887 2316 82-83 -0.21298847569610047 2316 84-85 -0.13793152809036968 2316 86-87 -0.16408165495797533 2316 88-89 -0.20917268097770148 2316 90-91 -0.3406351235231 2316 92-93 -0.2169131985732875 2316 94-95 -0.2343119773667297 2316 96-97 -0.22738940497363203 2316 98-99 -0.15992755944559534 2316 100 -0.3349187312927988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 39.0 14 251.0 15 774.0 16 2268.0 17 4947.0 18 9330.0 19 14491.0 20 21574.0 21 31524.0 22 40495.0 23 41312.0 24 39124.0 25 39625.0 26 45367.0 27 55724.0 28 68812.0 29 84555.0 30 105086.0 31 131850.0 32 168164.0 33 223329.0 34 333462.0 35 511368.0 36 911355.0 37 1623926.0 38 1528844.0 39 383568.0 40 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49042667113536 20.43115332837873 13.017640341855211 24.0607796586307 2 15.757913665310264 20.49065137748881 39.292146459182256 24.459288498018672 3 18.385552244477477 26.208227271983997 29.694675687818716 25.711544795719803 4 12.674022588538847 16.918121958425044 38.1647976296097 32.24305782342642 5 13.283436598300447 38.015836193931 34.856026317965764 13.844700889802786 6 30.96412684550087 37.525641257242306 17.457686085603957 14.052545811652859 7 27.091112535205454 32.25835839990282 22.774061376043704 17.876467688848017 8 26.425772401268183 34.866971724784854 19.794363770670344 18.912892103276622 9 26.472457496790717 14.78279598225945 20.87565589161314 37.8690906293367 10-11 25.661622010615165 25.66998482468497 27.576760938837232 21.091632225862632 12-13 25.878451030505794 23.483853746798637 27.917504407409822 22.720190815285747 14-15 23.240491437867362 24.72082101289175 25.45740004382332 26.581287505417567 16-17 22.41041130364771 27.479657514300087 26.5252620579354 23.584669124116804 18-19 22.01452014583218 27.153304985122368 28.59750952175495 22.234665347290502 20-21 23.280063077746778 26.30826679158461 28.10460590268423 22.30706422798438 22-23 23.54669371047654 26.02118985495826 27.843127422255016 22.588989012310186 24-25 23.008337359049328 26.162209412606767 28.32070177340187 22.50875145494204 26-27 22.965448438653656 26.311691179642548 27.737018422069422 22.985841959634374 28-29 22.440272260210907 26.67332006132419 28.08860565025668 22.79780202820822 30-31 23.057676076852225 26.328616677260513 28.00027969934812 22.613427546539143 32-33 23.201446523602797 26.61331516847038 27.399805145091534 22.78543316283529 34-35 23.215778248610665 26.52763599582324 27.963136432265085 22.29344932330101 36-37 23.07165792923694 26.177699728735277 27.76048997715944 22.990152364868337 38-39 23.10033218690269 26.223999450572936 28.09599151066668 22.57967685185769 40-41 22.83477154723929 25.77575792593197 28.473590661205666 22.915879865623076 42-43 22.81207439622179 25.97241094685039 27.92709107969522 23.288423577232606 44-45 22.875438081599146 26.552313875230872 27.763403581867752 22.80884446130223 46-47 22.93748040651355 26.36212204719443 27.332471700114265 23.36792584617776 48-49 23.42383839541708 26.23715698686858 27.349783333896617 22.98922128381772 50-51 23.115457287083306 26.261402663938892 27.14928885004333 23.473851198934476 52-53 22.678735358609952 26.61515988286187 27.117778920042646 23.58832583848553 54-55 22.672860939559897 26.63147476594323 26.97170293965801 23.72396135483886 56-57 23.327642453367552 26.29948096838661 26.776261204771618 23.596615373474215 58-59 22.616943820399708 26.516304115061303 27.293302756222822 23.573449308316167 60-61 22.72756970362376 26.84851336859751 26.850351153120233 23.57356577465849 62-63 22.915337712238177 26.407012331866497 26.643747913126248 24.033902042769075 64-65 22.361617622392913 26.49503519878127 26.89497427466901 24.24837290415681 66-67 22.80253995406678 26.211065429840957 26.9138542680096 24.07254034808266 68-69 22.50203954787874 26.225942114606042 27.03974449450554 24.232273843009672 70-71 22.469550445468013 26.009280012558573 26.99600941394575 24.525160128027668 72-73 22.80611410567694 26.10575013744201 27.065656007189038 24.02247974969202 74-75 22.865567028581054 26.466039835008342 26.726404201837788 23.941988934572812 76-77 22.66195910099211 26.438178782268185 26.447289496366732 24.452572620372973 78-79 22.54107692048931 26.453624007261034 26.516074132815376 24.48922493943428 80-81 22.287206275264175 26.782726390981797 26.745871610497062 24.184195723256963 82-83 22.784333840290742 26.071663598732066 27.36192483818491 23.782077722792284 84-85 22.981392673450337 26.38856865826366 26.459700737569968 24.17033793071603 86-87 22.038864080374136 26.896107121078956 26.791373805972075 24.273654992574837 88-89 22.531874969729465 26.16697872894436 26.68883105670366 24.61231524462251 90-91 22.435217744517036 26.144923284002086 26.680642283466906 24.739216688013975 92-93 22.672232110334196 26.262172201820476 26.503003835210087 24.56259185263524 94-95 21.927057186843456 26.18193911732526 27.00450939227664 24.88649430355465 96-97 22.11751054362775 26.180091282330327 26.697839041035625 25.0045591330063 98-99 21.733581230517203 26.598497277109978 26.57250529319978 25.095416199173037 100 21.88829317967368 26.382918948853117 26.094955048758887 25.633832822714307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 262.0 2 259.5 3 363.0 4 311.0 5 325.0 6 529.5 7 999.5 8 1612.0 9 2063.0 10 2059.5 11 1949.5 12 2209.0 13 2631.5 14 3088.5 15 3634.0 16 4243.0 17 4963.0 18 5777.5 19 6598.5 20 7558.5 21 8516.0 22 9595.0 23 11193.0 24 13139.5 25 15680.0 26 19184.5 27 23138.0 28 28868.0 29 38369.5 30 50863.5 31 63144.0 32 79343.5 33 95262.0 34 108602.0 35 123781.5 36 138868.5 37 162275.0 38 176691.0 39 176376.0 40 172988.5 41 168255.0 42 172666.5 43 184208.0 44 200336.5 45 229658.0 46 257583.5 47 277337.5 48 291786.0 49 292215.0 50 284268.0 51 285047.0 52 283819.5 53 274322.0 54 265865.0 55 240204.0 56 213025.0 57 182614.5 58 151944.5 59 138953.0 60 116524.5 61 88070.0 62 68525.0 63 54804.0 64 40177.5 65 25402.5 66 17147.0 67 12559.0 68 9584.0 69 7154.5 70 5411.0 71 4114.5 72 3095.5 73 2356.5 74 1764.0 75 1178.5 76 796.5 77 487.0 78 252.0 79 133.0 80 76.0 81 31.0 82 14.5 83 9.0 84 5.0 85 4.5 86 2.5 87 3.5 88 3.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005154723463102069 2 0.03292170816011412 3 0.0014015864401183873 4 0.001152415517430674 5 0.006758761277904223 6 4.671954800394624E-5 7 9.34390960078925E-4 8 0.00688334673924808 9 0.001152415517430674 10-11 1.7909160068179395E-4 12-13 1.0122568734188353E-4 14-15 8.720982294069965E-4 16-17 7.78659133399104E-5 18-19 0.0012224948394365936 20-21 2.0245137468376706E-4 22-23 0.001697476910810047 24-25 0.0011991350654346202 26-27 0.002149099208181527 28-29 1.7909160068179395E-4 30-31 0.0017831294154839483 32-33 0.0016663305454740825 34-35 7.786591333991041E-4 36-37 0.004064600676343323 38-39 9.42177551412916E-4 40-41 0.003924442032331484 42-43 0.004204759320355162 44-45 0.00404124090234135 46-47 0.0024294164962052047 48-49 0.009125885043437499 50-51 0.006649748999228349 52-53 0.0051157905064321135 54-55 0.004827686627074445 56-57 0.0031535694902663715 58-59 0.00576207758715337 60-61 0.008105841578684674 62-63 0.0032236488122722906 64-65 0.0029900510722525595 66-67 0.003644124744307807 68-69 0.0022036053475194646 70-71 0.002849892428240721 72-73 0.006307138980532743 74-75 0.0075607801853053 76-77 0.00441499728637292 78-79 0.002663014236224936 80-81 0.002289257852193366 82-83 0.004804326853072472 84-85 0.0022036053475194646 86-87 0.0034961795089619775 88-89 5.294882107113908E-4 90-91 0.0017364098674800023 92-93 6.229273067192832E-5 94-95 0.0022036053475194646 96-97 0.0018376355548218855 98-99 0.0013159339354444858 100 6.229273067192832E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6421295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.92919143477261 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91147103151748 41.65773371740881 2 15.99025954074708 18.52605612055273 3 5.415232435668771 9.411001092889384 4 2.4467907553721338 5.669624402751368 5 1.290340883908429 3.7374202040022544 6 0.7466057859145564 2.5950161699131917 7 0.4868514462791188 1.9742037442259293 8 0.33229934860298177 1.5399866063097896 9 0.24903644364615893 1.2983831836391968 >10 1.0903239858640892 10.468144232654106 >50 0.032760131429374256 1.260679349351734 >100 0.00714190168076909 0.6729404551869383 >500 5.113328444225924E-4 0.21267937895507028 >1k 2.7271019974094417E-4 0.2738562734756271 >5k 3.408877496107354E-5 0.1516794084458678 >10k+ 6.817754992214708E-5 0.550595660237949 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14869 0.23155765309022555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13013 0.20265382605845084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7681 0.11961761607277037 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.557318266798208E-5 0.0 3 0.0 0.0 0.0 1.557318266798208E-5 0.0 4 0.0 0.0 0.0 1.557318266798208E-5 0.0 5 0.0 0.0 0.0 1.557318266798208E-5 0.0 6 0.0 0.0 0.0 1.557318266798208E-5 0.0 7 0.0 0.0 0.0 1.557318266798208E-5 0.0 8 0.0 0.0 0.0 1.557318266798208E-5 0.0 9 0.0 0.0 0.0 1.557318266798208E-5 0.0 10-11 0.0 0.0 0.0 1.557318266798208E-5 0.0 12-13 0.0 4.671954800394624E-5 0.0 1.557318266798208E-5 0.0 14-15 0.0 6.229273067192832E-5 0.0 1.557318266798208E-5 3.114636533596416E-5 16-17 0.0 6.229273067192832E-5 0.0 1.557318266798208E-5 4.671954800394624E-5 18-19 0.0 6.229273067192832E-5 0.0 1.557318266798208E-5 4.671954800394624E-5 20-21 0.0 6.229273067192832E-5 0.0 6.229273067192832E-5 4.671954800394624E-5 22-23 0.0 7.78659133399104E-5 0.0 2.335977400197312E-4 5.450613933793728E-5 24-25 0.0 9.343909600789248E-5 0.0 6.307138980532743E-4 6.229273067192832E-5 26-27 0.0 9.343909600789248E-5 0.0 0.001713050093478029 6.229273067192832E-5 28-29 0.0 1.167988700098656E-4 0.0 0.0063850048938726535 6.229273067192832E-5 30-31 0.0 1.4015864401183874E-4 0.0 0.02215285234520451 6.229273067192832E-5 32-33 0.0 1.4015864401183874E-4 0.0 0.05744168427085191 6.229273067192832E-5 34-35 0.0 1.4794523534582976E-4 0.0 0.11621487565981628 9.343909600789248E-5 36-37 0.0 1.7130500934780287E-4 0.0 0.21167069882321243 1.1679887000986561E-4 38-39 0.0 1.9466478334977601E-4 0.0 0.34276575052228564 1.4015864401183874E-4 40-41 0.0 2.0245137468376706E-4 0.0 0.48030809984590334 1.4015864401183874E-4 42-43 0.0 2.0245137468376706E-4 0.0 0.620552396362416 1.4015864401183874E-4 44-45 0.0 2.1023796601775808E-4 0.0 0.7983592094741014 1.4015864401183874E-4 46-47 0.0 2.2581114868574018E-4 0.0 1.0155739613271155 1.4015864401183874E-4 48-49 0.0 2.3359774001973122E-4 0.0 1.2493507929475285 1.557318266798208E-4 50-51 0.0 2.3359774001973122E-4 0.0 1.4875737682196504 1.557318266798208E-4 52-53 0.0 2.4138433135372227E-4 0.0 1.7195207508765755 1.7909160068179395E-4 54-55 0.0 3.192502446936327E-4 0.0 1.9741111411327465 2.0245137468376706E-4 56-57 0.0 3.426100186956058E-4 0.0 2.2783410511431104 2.0245137468376706E-4 58-59 0.0 3.7375638403156994E-4 0.0 2.626487336277184 2.0245137468376706E-4 60-61 0.0 3.7375638403156994E-4 0.0 2.987170033458983 2.0245137468376706E-4 62-63 0.0 3.7375638403156994E-4 0.0 3.3535291557232614 2.0245137468376706E-4 64-65 0.0 3.7375638403156994E-4 0.0 3.7260786180980627 2.0245137468376706E-4 66-67 1.557318266798208E-5 4.049027493675341E-4 0.0 4.143774737027345 2.0245137468376706E-4 68-69 1.557318266798208E-5 4.049027493675341E-4 0.0 4.598830921177115 2.0245137468376706E-4 70-71 1.557318266798208E-5 4.2047593203551616E-4 0.0 5.095545368963737 2.0245137468376706E-4 72-73 1.557318266798208E-5 4.3604911470349826E-4 0.0 5.599034151210931 2.3359774001973122E-4 74-75 1.557318266798208E-5 4.3604911470349826E-4 0.0 6.088631654518286 2.3359774001973122E-4 76-77 1.557318266798208E-5 4.3604911470349826E-4 0.0 6.6246761751328975 2.491709226877133E-4 78-79 1.557318266798208E-5 4.5162229737148035E-4 0.0 7.199972902662157 2.491709226877133E-4 80-81 1.557318266798208E-5 4.5162229737148035E-4 0.0 7.812785427238587 2.5695751402170436E-4 82-83 1.557318266798208E-5 4.5162229737148035E-4 0.0 8.439271206197503 2.647441053556954E-4 84-85 1.557318266798208E-5 4.5162229737148035E-4 0.0 9.039041190289497 2.647441053556954E-4 86-87 1.557318266798208E-5 4.6719548003946245E-4 0.0 9.660761575352012 2.647441053556954E-4 88 1.557318266798208E-5 4.6719548003946245E-4 0.0 10.156362540577874 2.647441053556954E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10555 0.0 48.330788 1 GTATCAA 18095 0.0 39.208755 1 GTACTAG 4400 0.0 32.056858 1 CAACGCA 21270 0.0 31.95122 5 ATCAACG 21705 0.0 31.419872 3 GTGGTAT 3075 0.0 31.038683 1 TATCAAC 23240 0.0 30.445208 2 AACGCAG 23740 0.0 29.437206 6 TCAACGC 24550 0.0 27.854895 4 TAGGCAT 6110 0.0 26.691616 5 ACGCAGA 25625 0.0 26.409586 7 CTGTGCG 6200 0.0 25.696121 9 CGCAGAG 26410 0.0 25.482038 8 TAGTACT 6280 0.0 25.29572 4 GTACATG 24560 0.0 25.231289 1 TCTAACG 2165 0.0 24.755146 2 TACATGG 24715 0.0 24.7287 2 CTAACGC 2135 0.0 24.655678 3 TAACGCC 2255 0.0 24.38537 4 CTAGGCA 6680 0.0 24.1328 4 >>END_MODULE