FastQCFastQC Report
Thu 26 May 2016
ERR580202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7244301
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101950.1407313141737208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76840.10606958490543118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.045.9838521
GTATCAA168600.040.6902471
CAACGCA193550.034.0092855
ATCAACG197450.033.3870053
TATCAAC209300.032.3519822
AACGCAG215400.031.177566
TCAACGC216450.030.586214
ACGCAGA246950.026.5282967
CGCAGAG253700.025.5070428
GTACTAG33650.025.4319081
GTACATG291150.023.4822771
TACATGG293450.022.6420442
GCAGAGT284300.022.0874319
ACATGGG300400.021.4910283
GCATCGT32050.021.1145138
GTGGTAT44050.020.8152351
CGTTAAC9200.019.9328481
CATGGGG228050.019.8553164
GTGTAGC48200.019.5108221
GAGTACT110000.019.4658912-13
Produced by FastQC (version 0.11.5)