FastQCFastQC Report
Thu 26 May 2016
ERR580202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7244301
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92360.12749332199200447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.10271522400849993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.047.5699541
GTATCAA169600.041.9921261
CAACGCA198250.034.5393035
ATCAACG202700.033.7126463
TATCAAC211850.033.099362
AACGCAG221100.031.5844466
TCAACGC221050.031.1262474
ACGCAGA248300.027.424267
CGCAGAG257150.026.2976848
GTACTAG34850.024.0898461
GTACATG288250.023.4964311
TACATGG293000.022.6328162
GCAGAGT292600.022.549149
ACATGGG298700.021.304223
GTGGTAT44800.021.2662121
GAGTACT113850.019.8749712-13
CATGGGG226350.019.5797624
AGAGTAC263600.018.87033810-11
CAGAGTA282650.018.61269810-11
TGGTATC49950.018.253472
Produced by FastQC (version 0.11.5)