##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580202_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7244301
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54442340261676	34.0	31.0	34.0	31.0	34.0
2	32.74300211987326	34.0	31.0	34.0	31.0	34.0
3	32.82555915332618	34.0	31.0	34.0	31.0	34.0
4	36.28960516687531	37.0	37.0	37.0	35.0	37.0
5	36.22261043543055	37.0	37.0	37.0	35.0	37.0
6	36.249311838367845	37.0	37.0	37.0	35.0	37.0
7	36.21999707080089	37.0	37.0	37.0	35.0	37.0
8	36.209016301227685	37.0	37.0	37.0	35.0	37.0
9	37.99958325861943	39.0	38.0	39.0	35.0	39.0
10-11	37.951930351872456	39.0	38.0	39.0	35.0	39.0
12-13	37.918620305810045	39.0	38.0	39.0	35.0	39.0
14-15	39.32950563484317	41.0	39.0	41.0	36.0	41.0
16-17	39.31683146517518	41.0	39.0	41.0	36.0	41.0
18-19	39.31125183782397	41.0	39.0	41.0	36.0	41.0
20-21	39.29208856727516	41.0	39.0	41.0	36.0	41.0
22-23	39.22366650695491	41.0	39.0	41.0	36.0	41.0
24-25	39.14541175194128	40.5	39.0	41.0	35.5	41.0
26-27	39.01640151341034	40.0	38.5	41.0	35.0	41.0
28-29	38.91334678114562	40.0	38.0	41.0	35.0	41.0
30-31	38.81861769686268	40.0	38.0	41.0	35.0	41.0
32-33	38.69155264255309	40.0	38.0	41.0	35.0	41.0
34-35	38.67451014252445	40.0	38.0	41.0	35.0	41.0
36-37	38.73417255853947	40.0	38.0	41.0	35.0	41.0
38-39	38.65231158948255	40.0	38.0	41.0	35.0	41.0
40-41	38.54036531612918	40.0	38.0	41.0	34.0	41.0
42-43	38.52649565499833	40.0	38.0	41.0	34.0	41.0
44-45	38.45602646825415	40.0	38.0	41.0	34.0	41.0
46-47	38.29101896787557	40.0	38.0	41.0	34.0	41.0
48-49	38.11039298339481	40.0	37.5	41.0	33.5	41.0
50-51	37.9527592103089	40.0	37.0	41.0	33.0	41.0
52-53	37.7822732655642	40.0	37.0	41.0	33.0	41.0
54-55	37.58770156016433	40.0	36.0	41.0	33.0	41.0
56-57	37.377691857364844	39.0	36.0	41.0	33.0	41.0
58-59	37.151562241822916	39.0	35.0	41.0	32.0	41.0
60-61	36.890099127576285	39.0	35.0	41.0	32.0	41.0
62-63	36.59867908580828	38.0	35.0	40.0	31.5	41.0
64-65	36.302806578578114	37.5	35.0	40.0	31.0	41.0
66-67	35.968492543310944	37.0	35.0	40.0	31.0	41.0
68-69	35.6092824552707	36.5	35.0	39.0	31.0	41.0
70-71	35.269743347218736	36.0	34.5	39.0	30.0	40.5
72-73	34.905893888174994	35.5	34.0	38.5	30.0	40.0
74-75	34.54277127634536	35.0	34.0	37.0	30.0	39.0
76-77	33.301275927104626	34.5	32.5	36.0	28.0	39.0
78-79	33.9197018456301	35.0	34.0	36.0	29.5	39.0
80-81	33.84747934962945	35.0	34.0	36.0	30.0	37.0
82-83	33.639412691438416	35.0	34.0	36.0	29.5	37.0
84-85	33.42740065604673	35.0	34.0	35.0	29.0	37.0
86-87	33.26296898762213	35.0	34.0	35.0	29.0	36.0
88-89	33.11315432641465	35.0	34.0	35.0	29.0	36.0
90-91	32.96639599873059	35.0	33.0	35.0	29.0	36.0
92-93	32.86159651566107	35.0	33.0	35.0	29.0	35.0
94-95	32.7725121443739	35.0	33.0	35.0	29.0	35.0
96-97	32.68406296204424	35.0	33.0	35.0	29.0	35.0
98-99	32.618076195343065	35.0	33.0	35.0	29.0	35.0
100	31.351550135754987	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.16897585871745235
1101	2	0.10507216950067999
1101	3	0.0786645025867756
1101	4	0.053206553707752846
1101	5	9.243289966534007E-4
1101	6	0.033229141879282054
1101	7	0.01164222998182396
1101	8	0.05831657174040572
1101	9	0.036820428488034906
1101	10-11	0.051540540352498
1101	12-13	0.011622399555491825
1101	14-15	0.030942690131439576
1101	16-17	-0.014034369639503552
1101	18-19	-0.018674089129177673
1101	20-21	0.019919435562002263
1101	22-23	0.036467861845785876
1101	24-25	-0.030453798228379014
1101	26-27	-0.04715131252567062
1101	28-29	-0.010207869822920657
1101	30-31	-0.02041891272907037
1101	32-33	-0.012785647424827573
1101	34-35	-0.10974385681668508
1101	36-37	-0.01525537061522897
1101	38-39	0.022161009759436467
1101	40-41	0.03661813735091357
1101	42-43	-0.04609610405974962
1101	44-45	-0.0761230822849015
1101	46-47	0.007301842831239469
1101	48-49	-0.042803401396817264
1101	50-51	-0.022133865545534093
1101	52-53	-0.017357854927965377
1101	54-55	0.039820598806784346
1101	56-57	-0.056899936528772344
1101	58-59	0.018210054043024115
1101	60-61	-0.042331370448984273
1101	62-63	-0.06048270661484878
1101	64-65	-0.07724140461159834
1101	66-67	-0.09176535470727032
1101	68-69	-0.08120011104090707
1101	70-71	-0.12119606664828808
1101	72-73	-0.09292001141228923
1101	74-75	-0.06366066206494025
1101	76-77	-0.09212579966635559
1101	78-79	-0.08585127221854094
1101	80-81	-0.11998160016094772
1101	82-83	-0.045527013691824436
1101	84-85	-0.08970081659222018
1101	86-87	-0.0714362070164114
1101	88-89	-0.11398954530388039
1101	90-91	-0.1429247442919248
1101	92-93	-0.14268320048201133
1101	94-95	-0.1253484192608667
1101	96-97	-0.09395546206991412
1101	98-99	-0.1453316088772283
1101	100	-0.14320542961340266
1102	1	0.10751989028053544
1102	2	0.03262820616556894
1102	3	0.0060343700811813505
1102	4	-0.009570113858259788
1102	5	0.012249632364458307
1102	6	0.009275953026168793
1102	7	0.028113220760133117
1102	8	0.012728843113023913
1102	9	0.010219765883419996
1102	10-11	0.014791131061947738
1102	12-13	-0.009976271090081923
1102	14-15	0.023466786486643798
1102	16-17	0.0025386912509546278
1102	18-19	-0.010935196900696553
1102	20-21	0.002077235134358091
1102	22-23	-0.016573940613326954
1102	24-25	-0.011962687678554573
1102	26-27	-0.06686750160261568
1102	28-29	-0.0890070089784274
1102	30-31	-0.04870459424725482
1102	32-33	-0.11776136880481403
1102	34-35	-0.04336695773241672
1102	36-37	-0.019078543546534377
1102	38-39	-0.08553785536760472
1102	40-41	-0.04336702749856158
1102	42-43	-0.06624389034337952
1102	44-45	-0.05673626522234798
1102	46-47	-0.030203080507710922
1102	48-49	-0.06835772904185689
1102	50-51	-0.03602848226437061
1102	52-53	-0.02018892534376704
1102	54-55	-0.09077332499930435
1102	56-57	-0.1028488194809043
1102	58-59	-0.09401701491057679
1102	60-61	-0.07736389641433306
1102	62-63	-0.09999324516920183
1102	64-65	-0.08281794119508135
1102	66-67	-0.11470432843859157
1102	68-69	-0.06999853427342373
1102	70-71	-0.03074608350276975
1102	72-73	-0.03978419388698029
1102	74-75	-0.07040705039398176
1102	76-77	-0.09206151437900445
1102	78-79	-0.04952820565397076
1102	80-81	-0.07667666972844245
1102	82-83	-0.05711079007396336
1102	84-85	-0.06476618926517119
1102	86-87	-0.11329579948326085
1102	88-89	-0.04342736111550494
1102	90-91	0.015821902601345528
1102	92-93	-0.05174663052964945
1102	94-95	-0.0933915433678365
1102	96-97	-0.15314313686806713
1102	98-99	-0.1446083332573096
1102	100	-0.026587047002404773
1103	1	0.09019170548891964
1103	2	0.03776516697080723
1103	3	0.029110491402796868
1103	4	0.007741692217749119
1103	5	-0.04611964315034811
1103	6	-0.01679126919329832
1103	7	-0.005211261449105109
1103	8	-0.007518723655060455
1103	9	0.05233242166734442
1103	10-11	0.009673651001797623
1103	12-13	-0.011690824819709178
1103	14-15	0.010779485947146838
1103	16-17	-0.0255888989436599
1103	18-19	-0.028229507530475928
1103	20-21	-0.03993528713821348
1103	22-23	-0.09306762984457606
1103	24-25	-0.06782515113665255
1103	26-27	-0.06529576530985537
1103	28-29	-0.11676731888357494
1103	30-31	-0.14675738830725038
1103	32-33	-0.14277948465693413
1103	34-35	-0.12902366977970559
1103	36-37	-0.11621512218174246
1103	38-39	-0.15179224163727412
1103	40-41	-0.14692592387092418
1103	42-43	-0.11901995855824765
1103	44-45	-0.07612382533967121
1103	46-47	-0.09040267749203679
1103	48-49	-0.0690850929124025
1103	50-51	-0.04690171596879367
1103	52-53	-0.09354646277906653
1103	54-55	-0.04921022475659953
1103	56-57	-0.09237810512074418
1103	58-59	-0.18328039458409506
1103	60-61	-0.11977645640758539
1103	62-63	-0.11516837334505681
1103	64-65	-0.07248396429402959
1103	66-67	-0.08298756606397717
1103	68-69	-0.08731369054125793
1103	70-71	-0.08680806949648456
1103	72-73	-0.11796116839077087
1103	74-75	-0.17790204992247283
1103	76-77	-0.2223600288791232
1103	78-79	-0.08589751591151895
1103	80-81	-0.04377604882623132
1103	82-83	0.03888486212152742
1103	84-85	-0.024313491492172545
1103	86-87	-0.052566872189864
1103	88-89	-0.030403304256552133
1103	90-91	-0.022098918716793037
1103	92-93	-0.03162401350869004
1103	94-95	-0.14116850496719024
1103	96-97	-0.07557080994889986
1103	98-99	-0.07017997524837227
1103	100	0.033746185411569485
1104	1	0.14478778497252875
1104	2	0.0820574305976578
1104	3	0.038743648957009214
1104	4	0.06694957537403923
1104	5	0.01237250088610864
1104	6	0.034368029103944764
1104	7	0.05521939379516283
1104	8	0.007661610433466137
1104	9	0.02957302844792764
1104	10-11	0.007152948087274069
1104	12-13	0.041521706349158194
1104	14-15	0.05839781227310681
1104	16-17	0.02490599826847273
1104	18-19	0.030122399099575148
1104	20-21	-0.02560164292756184
1104	22-23	-0.03607765076928615
1104	24-25	-0.0010852371580583053
1104	26-27	0.010686635860366778
1104	28-29	-0.03223063427834916
1104	30-31	-0.07094860238053968
1104	32-33	-0.10553942966554786
1104	34-35	-0.052206462035044865
1104	36-37	-0.03369670576626049
1104	38-39	-0.020722199751787684
1104	40-41	-0.010138480285057483
1104	42-43	0.021281282282231473
1104	44-45	-0.04638227907485515
1104	46-47	-0.04467581603296367
1104	48-49	0.010215184280532696
1104	50-51	-0.04136146384800554
1104	52-53	-0.08438632922443645
1104	54-55	-0.17087682290042494
1104	56-57	-0.062421884004024264
1104	58-59	-0.024545517395061722
1104	60-61	-0.07579386193081206
1104	62-63	-0.05393609100786989
1104	64-65	-0.08185195648098187
1104	66-67	-0.08696354988969546
1104	68-69	-0.08870266658639281
1104	70-71	-0.0675818582831198
1104	72-73	-0.12047040274539
1104	74-75	-0.11753925032437706
1104	76-77	-0.18334676502073677
1104	78-79	-0.01879830781918912
1104	80-81	-0.023081176715095353
1104	82-83	-0.013527682896182114
1104	84-85	-0.04197988839523248
1104	86-87	-0.028155086693779197
1104	88-89	-0.025154021762830325
1104	90-91	0.012836226216229818
1104	92-93	-0.05663795249739678
1104	94-95	-0.04594205861229028
1104	96-97	0.016376367595228203
1104	98-99	-0.010511077622219034
1104	100	0.02843043576085691
1105	1	0.053245256211248204
1105	2	0.002744889720709409
1105	3	-0.01736364277872582
1105	4	-0.031479316740146146
1105	5	-0.06602649703933139
1105	6	-0.028929337988678583
1105	7	-0.019550978704984345
1105	8	-0.007007263615548709
1105	9	-0.03524246103932427
1105	10-11	-0.02509790796265321
1105	12-13	-0.04243672011631361
1105	14-15	-0.01614980891574902
1105	16-17	-0.04975565167980989
1105	18-19	-0.03245537513343777
1105	20-21	-0.0689791786109879
1105	22-23	-0.05424331560207918
1105	24-25	-0.10199409751815125
1105	26-27	-0.037115921359699655
1105	28-29	-0.06519134602349652
1105	30-31	-0.08252693874332806
1105	32-33	-0.03840821820256224
1105	34-35	-0.0755440381462904
1105	36-37	-0.11019126750525032
1105	38-39	-0.07916572715227232
1105	40-41	-0.08433701318978137
1105	42-43	-0.07054192844817209
1105	44-45	-0.08846816349056041
1105	46-47	-0.1348269969978304
1105	48-49	-0.11822848972445854
1105	50-51	-0.036465858284998376
1105	52-53	-0.08115922507244733
1105	54-55	-0.06351162081269024
1105	56-57	-0.04601003017702254
1105	58-59	-0.052678510212096796
1105	60-61	-0.08253840731354245
1105	62-63	-0.09722306559211802
1105	64-65	-0.06108436268309703
1105	66-67	-0.08576992670396066
1105	68-69	-0.09971564567950253
1105	70-71	-0.11093417580482878
1105	72-73	-0.1604139468850505
1105	74-75	-0.20391394319342737
1105	76-77	-0.16679128801893484
1105	78-79	-0.08524977768244213
1105	80-81	-0.07303080603547585
1105	82-83	-0.0637344171190719
1105	84-85	-0.05003643462521268
1105	86-87	-0.052349724732742686
1105	88-89	-0.03308877138314159
1105	90-91	-0.03522012636452132
1105	92-93	-0.05696595270920568
1105	94-95	-0.055553077493847525
1105	96-97	-0.07935967888408868
1105	98-99	-0.09088486001169116
1105	100	0.016258487940678634
1106	1	0.11615297536981473
1106	2	0.06653588902572949
1106	3	0.01785907135484166
1106	4	0.03311230585497782
1106	5	-0.027198972401855315
1106	6	0.032574429071786426
1106	7	0.0288334045295926
1106	8	0.0168709809949803
1106	9	-0.01159192760358252
1106	10-11	0.0016829594923493119
1106	12-13	-0.022288918175028982
1106	14-15	0.037711492695315485
1106	16-17	-0.00899694307064891
1106	18-19	0.016375156395596946
1106	20-21	-0.040620212911761655
1106	22-23	-0.016403109816685912
1106	24-25	-0.007471170462217458
1106	26-27	-0.07229790509444456
1106	28-29	-0.030805008134592526
1106	30-31	0.0020362419440189683
1106	32-33	0.0032634788109646706
1106	34-35	-0.011035431225110415
1106	36-37	0.022633295580490653
1106	38-39	0.015496549942170645
1106	40-41	0.03211001937847158
1106	42-43	0.0038360488064412834
1106	44-45	0.07041930981686306
1106	46-47	0.037339377563696985
1106	48-49	0.09458725528054401
1106	50-51	-0.03759231556521314
1106	52-53	-0.005698934303950409
1106	54-55	0.032793093283373764
1106	56-57	-0.035716795042681326
1106	58-59	-0.03851434151401634
1106	60-61	-0.010185357431033992
1106	62-63	-0.05654916674494359
1106	64-65	-0.030391914069603843
1106	66-67	-0.053519280422840154
1106	68-69	-0.01842416394880786
1106	70-71	-0.03239893773514524
1106	72-73	0.01742622651475756
1106	74-75	0.007515606584867385
1106	76-77	-0.09954639321607317
1106	78-79	0.0420487458162242
1106	80-81	0.07509485314237452
1106	82-83	0.10089445994429269
1106	84-85	0.06190971866509187
1106	86-87	0.12481610157389156
1106	88-89	0.1437283670118319
1106	90-91	0.1356329205462501
1106	92-93	-0.01871253624574365
1106	94-95	0.08474944696835252
1106	96-97	0.08469820895832214
1106	98-99	0.10334184194717722
1106	100	0.07216131453835217
1107	1	0.11775682844896807
1107	2	0.08220267648710688
1107	3	0.0424017286037639
1107	4	0.01899640947192438
1107	5	0.03049863141995246
1107	6	0.028784673093561253
1107	7	0.014244777328329405
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2316	1	0.0994225598536147
2316	2	-0.03150808619861323
2316	3	-0.0168716530604911
2316	4	-0.1681088408596736
2316	5	-0.1431446683475528
2316	6	-0.05666065661554143
2316	7	-0.04433978338271061
2316	8	0.01271896183757093
2316	9	-0.0111627008768167
2316	10-11	-0.019115639376728666
2316	12-13	0.017211632402428734
2316	14-15	0.006972365216249443
2316	16-17	-0.016555536182892183
2316	18-19	-0.005027957476237077
2316	20-21	-0.0015283826501359954
2316	22-23	-0.05028475513776698
2316	24-25	-0.012763249875426652
2316	26-27	0.0016354541594623129
2316	28-29	0.04619629383448398
2316	30-31	-0.27993644890337777
2316	32-33	-0.12627583386765906
2316	34-35	-0.189235197807065
2316	36-37	-0.4462695323821251
2316	38-39	-0.2825246592308943
2316	40-41	-0.25112138907650916
2316	42-43	-0.3211340909734801
2316	44-45	-0.2061421807944086
2316	46-47	-0.3352216559426253
2316	48-49	-0.342353878342891
2316	50-51	-0.3866148575937842
2316	52-53	-0.4311578157494509
2316	54-55	-0.2952266165168993
2316	56-57	-1.0342619154691803
2316	58-59	-0.8644548061824722
2316	60-61	-0.45370625064796855
2316	62-63	-0.21650743580517684
2316	64-65	-0.1210475737227128
2316	66-67	-0.11663915883740117
2316	68-69	-0.13662815353969648
2316	70-71	-0.1418377964586668
2316	72-73	-0.042227211467668724
2316	74-75	-0.04890454317366988
2316	76-77	-0.058291088504880406
2316	78-79	-0.14934916546109633
2316	80-81	-0.10330341899530993
2316	82-83	-0.2367972013235189
2316	84-85	-0.08282902450022078
2316	86-87	-0.2023527452055518
2316	88-89	-0.18072425916507484
2316	90-91	-0.12253628702359975
2316	92-93	-0.11085646288256612
2316	94-95	-0.05567086467276994
2316	96-97	-0.17956754146499776
2316	98-99	-0.052742473138962964
2316	100	-0.38349108393956755
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	11.0
13	52.0
14	226.0
15	694.0
16	1495.0
17	3194.0
18	5631.0
19	9017.0
20	13826.0
21	20802.0
22	27612.0
23	30853.0
24	31917.0
25	35014.0
26	42203.0
27	52698.0
28	65694.0
29	81999.0
30	101897.0
31	127163.0
32	161727.0
33	213108.0
34	315499.0
35	484583.0
36	869560.0
37	1864643.0
38	2282611.0
39	400518.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09273363629589	19.42423909125641	13.76665862300445	23.716368649443258
2	15.923749233012918	20.545107813018728	39.025476049642094	24.505666904326258
3	19.202933561415783	25.282489463242413	29.661835375344896	25.85274159999691
4	13.444891177679672	17.194776948270572	38.402118993500174	30.958212880549585
5	14.27587750158857	37.587160701203366	33.86336205938558	14.273599737822487
6	30.868140349220717	36.53913607399803	18.42999069199361	14.162732884787644
7	28.078966381559678	32.29077544849756	21.523935100405865	18.1063230695369
8	26.982863150362107	33.94780041389942	19.982242522091937	19.087093913646534
9	26.480805886614423	16.404103424361026	20.287997287722305	36.82709340130225
10-11	25.481171669407214	25.750680259337827	27.68202044753555	21.086127623719406
12-13	26.391456617529624	23.032918169975638	27.670918240928	22.90470697156674
14-15	23.766816545856393	24.69011063764938	25.28682288465146	26.256249931842767
16-17	23.383670662110898	27.527422450156454	25.857701079850102	23.23120580788255
18-19	22.941164737120985	27.096772323318312	27.537598687949888	22.424464251610814
20-21	24.14991551955474	26.139657771372725	27.259410182930534	22.451016526142002
22-23	23.974333437001203	25.8967236122137	26.989543073171156	23.13939987761394
24-25	23.60194586061512	26.020295125782322	27.23897032991865	23.138788683683906
26-27	23.50271846488248	26.36317354581524	26.838611560591826	23.295496428710457
28-29	23.26262904324455	26.465775808441826	27.05908688918703	23.212508259126594
30-31	23.927962628064662	26.074732689075464	27.0598935360083	22.937411146851577
32-33	23.74806037084736	26.25344101031977	26.69925112487964	23.29924749395323
34-35	23.845931047640768	26.419140244325863	26.940274780655766	22.794653927377603
36-37	23.746504853048688	26.200340128611604	26.737957874000827	23.315197144338878
38-39	23.709046334432472	26.271594701642435	26.900172563643842	23.11918640028125
40-41	23.879526137872624	25.803137106611384	27.063772666455023	23.253564089060966
42-43	23.62124613120629	25.801768670294567	26.94040194040194	23.6365832580972
44-45	23.69160843977798	26.124962539094966	26.86627088441142	23.317158136715634
46-47	23.58420676380267	25.98949308856274	26.51662637183555	23.90967377579904
48-49	23.833711010378824	25.948877336919864	26.774180143529243	23.443231509172072
50-51	23.796138114147862	26.052485960377282	26.609881224615812	23.54149470085904
52-53	23.615516737268656	26.14704881104003	26.49895208295428	23.738482368737035
54-55	23.473350965588942	26.01743586104818	26.66474413511688	23.844469038246
56-57	23.844570476333317	26.022307372851692	26.35708154281308	23.776040608001907
58-59	23.38462980451904	26.210998348278615	26.782878666497428	23.62149318070492
60-61	23.672798795021148	26.257135546938333	26.528908718489692	23.54115693955083
62-63	23.64718215087288	26.0766049564129	26.420126580723334	23.85608631199088
64-65	23.2716135913328	25.968279656835392	26.65328231099895	24.10682444083286
66-67	23.50445912302039	25.994846769347063	26.52557527625401	23.975118831378538
68-69	23.270225679034567	26.005351604915504	26.649896427229464	24.074526288820465
70-71	23.305933842975403	25.749987800643392	26.578646784556742	24.365431571824463
72-73	23.275028276414574	25.991676135851854	26.56044153931093	24.17285404842264
74-75	23.337012590209497	26.092127386544302	26.528330197417404	24.042529825828797
76-77	23.158559457838184	26.009469996458467	26.34062514731945	24.4913453983839
78-79	23.104183133895237	25.95861702060107	26.526058276250776	24.411141569252916
80-81	23.116797603620796	26.322106632662745	26.372843336364465	24.18825242735199
82-83	23.40755270702857	26.002989941043218	26.738037820131446	23.851419531796765
84-85	23.404159130447084	26.046168770596267	26.38726315909352	24.162408939863127
86-87	22.848055278101935	26.29103706974375	26.613374864982227	24.247532787172087
88-89	23.05559934546131	25.812976088686845	26.653001455235962	24.47842311061588
90-91	23.161168166364327	25.848455753483755	26.57014793782168	24.420228142330235
92-93	23.101156939709114	25.897087092772527	26.655629721867474	24.34612624565089
94-95	22.743757985404763	25.87749119953264	26.7896641883426	24.58908662672
96-97	22.70684407552252	25.89166696173151	26.750999303508937	24.65048965923704
98-99	22.504039124567374	25.875189670442772	26.735367215229296	24.885403989760558
100	22.744574557813173	25.74001499148964	26.50971616941212	25.005694281285074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	77.0
2	108.0
3	175.0
4	136.5
5	117.5
6	280.5
7	573.0
8	928.0
9	1113.0
10	1036.5
11	979.5
12	1112.5
13	1317.0
14	1554.0
15	1803.0
16	2077.0
17	2415.0
18	2767.0
19	3188.5
20	3688.5
21	4216.0
22	4812.0
23	5683.0
24	6798.5
25	8217.5
26	10153.5
27	12705.0
28	15867.5
29	20461.5
30	26981.0
31	34566.5
32	45580.0
33	60136.5
34	76218.5
35	95063.0
36	120915.5
37	152771.0
38	180684.5
39	203550.5
40	221169.5
41	233080.0
42	247629.5
43	269067.0
44	290444.5
45	315355.0
46	337685.5
47	354025.5
48	367272.0
49	368787.5
50	358215.0
51	346542.5
52	333458.0
53	317276.5
54	300903.0
55	271394.5
56	237787.0
57	201635.0
58	167114.5
59	143384.0
60	116179.0
61	88957.0
62	69092.0
63	53008.0
64	37720.0
65	25011.5
66	17252.5
67	12070.5
68	8853.0
69	6660.0
70	4928.0
71	3634.0
72	2586.0
73	1805.5
74	1334.0
75	897.0
76	537.5
77	293.0
78	158.0
79	79.5
80	44.0
81	30.5
82	19.5
83	10.0
84	8.5
85	9.5
86	8.5
87	5.0
88	2.0
89	1.5
90	1.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3501649089401448
2	6.349818981845177E-4
3	0.001891141740245194
4	0.0014770231109944216
5	0.004886599825159115
6	0.0
7	4.141186292507724E-5
8	4.003146749424134E-4
9	0.0028298106332136116
10-11	6.90197715417954E-5
12-13	5.5215817233436325E-5
14-15	3.036869947838998E-4
16-17	1.173336116210522E-4
18-19	9.041590071975198E-4
20-21	2.8988304047554073E-4
22-23	1.6564745170030896E-4
24-25	0.0
26-27	0.0019187496488619123
28-29	0.011029359492378906
30-31	0.0035269103257857455
32-33	0.01339673765626249
34-35	0.010739476451903365
36-37	0.011933518499576426
38-39	0.012237205494360326
40-41	0.01339673765626249
42-43	0.006087543849986355
44-45	0.0011319242532854446
46-47	0.0055560916091145305
48-49	0.012409754923214814
50-51	0.0034440865999355906
52-53	0.005618209403502146
54-55	0.005245502637176451
56-57	2.001573374712067E-4
58-59	5.590601494885429E-4
60-61	3.381968805547975E-4
62-63	9.662768015851357E-4
64-65	3.105889719380793E-4
66-67	0.0014839250881486014
68-69	0.0016426705626947306
70-71	9.179629615058789E-4
72-73	0.002615849341434046
74-75	0.007419625440743006
76-77	0.0036511459145609766
78-79	0.0015874547454612943
80-81	5.314522408718246E-4
82-83	1.518434973919499E-4
84-85	0.0016357685855405513
86-87	0.004796874122154781
88-89	0.0015874547454612943
90-91	1.035296573126931E-4
92-93	0.0020222793061746057
94-95	0.003112791696534973
96-97	0.00180141603724086
98-99	0.0021672208264123757
100	0.0026365552728965843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7244301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.59561072862347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28048859986572	45.01071571929417
2	14.891884056460256	18.047656186021197
3	5.04281912992414	9.167181149152166
4	2.223064136627979	5.388317161914897
5	1.1267623067465493	3.4138425061649866
6	0.6343877536383947	2.306466802228683
7	0.40996359018827316	1.7389395886770262
8	0.27801952919117406	1.347741053265884
9	0.1941721340479866	1.0589381144206105
>10	0.8671273034761706	8.701649216343526
>50	0.035458012606473736	1.4621437090564495
>100	0.014912913878612608	1.5896089839012508
>500	6.639140060565927E-4	0.24753235267138987
>1k	2.212954737172853E-4	0.23972895087697857
>5k	5.532386841210237E-5	0.27953850601081753
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9236	0.12749332199200447	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7441	0.10271522400849993	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.7607908616718162E-5	0.0
4	0.0	0.0	0.0	5.5215817233436325E-5	1.3803954308359081E-5
5	0.0	0.0	0.0	6.90197715417954E-5	1.3803954308359081E-5
6	0.0	0.0	0.0	6.90197715417954E-5	1.3803954308359081E-5
7	0.0	0.0	0.0	9.662768015851357E-5	1.3803954308359081E-5
8	0.0	0.0	0.0	1.1043163446687265E-4	1.3803954308359081E-5
9	0.0	0.0	0.0	2.484711775504635E-4	1.3803954308359081E-5
10-11	0.0	0.0	0.0	3.381968805547975E-4	1.3803954308359081E-5
12-13	0.0	0.0	0.0	4.2792258355913153E-4	1.3803954308359081E-5
14-15	0.0	0.0	0.0	4.8313840079256784E-4	2.7607908616718162E-5
16-17	0.0	0.0	0.0	5.659621266427223E-4	3.45098857708977E-5
18-19	0.0	0.0	0.0	6.418838753386974E-4	5.5215817233436325E-5
20-21	0.0	0.0	0.0	8.006293498848268E-4	5.5215817233436325E-5
22-23	0.0	0.0	0.0	0.0011319242532854446	5.5215817233436325E-5
24-25	0.0	0.0	0.0	0.001621964631232192	5.5215817233436325E-5
26-27	0.0	0.0	0.0	0.0021189069863331187	5.5215817233436325E-5
28-29	0.0	0.0	0.0	0.003623538005944259	5.5215817233436325E-5
30-31	0.0	0.0	0.0	0.008972570300433403	5.5215817233436325E-5
32-33	0.0	1.3803954308359081E-5	0.0	0.022293386207999914	8.28237258501545E-5
34-35	0.0	1.3803954308359081E-5	0.0	0.04409673203805309	8.28237258501545E-5
36-37	0.0	1.3803954308359081E-5	0.0	0.08056677932073777	8.28237258501545E-5
38-39	0.0	1.3803954308359081E-5	0.0	0.1376461303858026	8.28237258501545E-5
40-41	0.0	1.3803954308359081E-5	0.0	0.20276628483548653	8.28237258501545E-5
42-43	0.0	1.3803954308359081E-5	0.0	0.27224158686945776	8.972570300433403E-5
44-45	0.0	1.3803954308359081E-5	0.0	0.3624780361832011	9.662768015851357E-5
46-47	0.0	1.3803954308359081E-5	0.0	0.4676089521956639	1.0352965731269312E-4
48-49	0.0	1.3803954308359081E-5	0.0	0.5852393488343458	1.380395430835908E-4
50-51	0.0	1.3803954308359081E-5	0.0	0.7099787819418326	1.380395430835908E-4
52-53	0.0	1.3803954308359081E-5	0.0	0.8380794779234049	1.4494152023777034E-4
54-55	0.0	1.3803954308359081E-5	0.0	0.9827104091892371	1.518434973919499E-4
56-57	0.0	1.3803954308359081E-5	0.0	1.1488409992903388	1.5874547454612946E-4
58-59	0.0	1.3803954308359081E-5	0.0	1.3388110184819764	1.7254942885448852E-4
60-61	0.0	1.3803954308359081E-5	0.0	1.54576680345005	1.7945140600866805E-4
62-63	0.0	1.3803954308359081E-5	0.0	1.7570639320481023	1.7945140600866805E-4
64-65	0.0	1.3803954308359081E-5	0.0	1.976436373916545	1.8635338316284758E-4
66-67	0.0	1.3803954308359081E-5	0.0	2.2218430736105526	2.0705931462538623E-4
68-69	0.0	1.3803954308359081E-5	0.0	2.499985850946834	2.208632689337453E-4
70-71	0.0	1.3803954308359081E-5	0.0	2.8085870534645094	2.208632689337453E-4
72-73	0.0	1.3803954308359081E-5	0.0	3.1226201120025245	2.760790861671816E-4
74-75	0.0	2.7607908616718162E-5	0.0	3.4384407826234717	2.760790861671816E-4
76-77	0.0	2.7607908616718162E-5	0.0	3.7835051304466782	2.760790861671816E-4
78-79	0.0	2.7607908616718162E-5	0.0	4.163286423355407	2.760790861671816E-4
80-81	0.0	2.7607908616718162E-5	0.0	4.569253817587094	2.8298106332136114E-4
82-83	0.0	2.7607908616718162E-5	0.0	4.992779841699013	2.8988304047554073E-4
84-85	0.0	2.7607908616718162E-5	0.0	5.423104313307799	2.8988304047554073E-4
86-87	0.0	2.7607908616718162E-5	0.0	5.871429141334685	2.8988304047554073E-4
88	0.0	2.7607908616718162E-5	0.0	6.2319470160060995	2.8988304047554073E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9310	0.0	47.569954	1
GTATCAA	16960	0.0	41.992126	1
CAACGCA	19825	0.0	34.539303	5
ATCAACG	20270	0.0	33.712646	3
TATCAAC	21185	0.0	33.09936	2
AACGCAG	22110	0.0	31.584446	6
TCAACGC	22105	0.0	31.126247	4
ACGCAGA	24830	0.0	27.42426	7
CGCAGAG	25715	0.0	26.297684	8
GTACTAG	3485	0.0	24.089846	1
GTACATG	28825	0.0	23.496431	1
TACATGG	29300	0.0	22.632816	2
GCAGAGT	29260	0.0	22.54914	9
ACATGGG	29870	0.0	21.30422	3
GTGGTAT	4480	0.0	21.266212	1
GAGTACT	11385	0.0	19.87497	12-13
CATGGGG	22635	0.0	19.579762	4
AGAGTAC	26360	0.0	18.870338	10-11
CAGAGTA	28265	0.0	18.612698	10-11
TGGTATC	4995	0.0	18.25347	2
>>END_MODULE