FastQCFastQC Report
Thu 26 May 2016
ERR580201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805610
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263180.33716775498647766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218340.2797218923312848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.15637470998422928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146700.052.154031
GTATCAA269200.041.4177931
CAACGCA308050.035.081515
ATCAACG316100.034.2163053
TATCAAC337600.032.8644072
GTACTAG63450.032.091261
AACGCAG347700.031.9568636
GTGGTAT42400.030.721641
TCAACGC368250.029.640124
ACGCAGA368750.029.1013347
CGCAGAG372250.028.8149098
TAGGCAT80650.027.6853335
GCATCGT69600.026.8670438
GGGCACC72100.026.72942294
GCAGAGT397650.026.4340679
CTAGGCA87550.025.8792324
GTACATG362950.025.8479791
AGGGCTA84950.025.8412785
GCTATTG84450.025.3694618
TACATGG370800.025.1039262
Produced by FastQC (version 0.11.5)