FastQCFastQC Report
Thu 26 May 2016
ERR580201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7805610
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226760.290509005702309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199200.25520106692494243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114660.1468943490643268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149700.052.013681
GTATCAA268100.040.092071
CAACGCA311250.033.3432045
ATCAACG313750.033.063813
TATCAAC341350.031.3791522
GTGGTAT43100.030.655481
AACGCAG350100.030.4054876
TCAACGC365900.028.6077564
GTACTAG63150.028.2192151
GGGCACC70750.027.90054394
ACGCAGA373300.027.7478627
CGCAGAG380350.027.1475648
CTAACGC32350.025.863073
TAGTACT87400.025.760254
GTACATG359250.025.6660981
CTGTGCG85200.025.5934739
TAACGCC33100.025.2765624
TCTAACG33500.025.2647532
TAGGCAT80950.025.1413565
GCTATTG81350.025.1313578
Produced by FastQC (version 0.11.5)