##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580201_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7805610
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54358442197343	34.0	31.0	34.0	31.0	34.0
2	32.66322286150602	34.0	31.0	34.0	31.0	34.0
3	32.710214192100295	34.0	31.0	34.0	31.0	34.0
4	36.225492434287645	37.0	35.0	37.0	35.0	37.0
5	36.173897107336906	37.0	35.0	37.0	35.0	37.0
6	36.21200867068685	37.0	37.0	37.0	35.0	37.0
7	36.16010894728279	37.0	36.0	37.0	35.0	37.0
8	36.15954281600029	37.0	37.0	37.0	35.0	37.0
9	37.87337812163303	39.0	38.0	39.0	35.0	39.0
10-11	37.826535402101825	39.0	38.0	39.0	35.0	39.0
12-13	37.785806234234094	39.0	38.0	39.0	35.0	39.0
14-15	39.15962205388176	41.0	39.0	41.0	36.0	41.0
16-17	39.15724325196878	40.0	39.0	41.0	36.0	41.0
18-19	39.15488007984001	40.0	39.0	41.0	36.0	41.0
20-21	39.12676068878665	40.0	39.0	41.0	35.0	41.0
22-23	39.046089863572476	40.0	38.5	41.0	35.0	41.0
24-25	38.96859316568468	40.0	38.0	41.0	35.0	41.0
26-27	38.833915734964975	40.0	38.0	41.0	35.0	41.0
28-29	38.74129766924045	40.0	38.0	41.0	35.0	41.0
30-31	38.58654627889428	40.0	38.0	41.0	34.5	41.0
32-33	38.475810154491455	40.0	38.0	41.0	34.0	41.0
34-35	38.37934946788272	40.0	38.0	41.0	34.0	41.0
36-37	38.258459044200265	40.0	38.0	41.0	34.0	41.0
38-39	38.13539748975417	40.0	37.5	41.0	34.0	41.0
40-41	38.014333793258956	40.0	37.0	41.0	33.0	41.0
42-43	38.000069181012115	40.0	37.0	41.0	33.0	41.0
44-45	37.93294117948501	40.0	37.0	41.0	33.0	41.0
46-47	37.71860085246381	40.0	37.0	41.0	33.0	41.0
48-49	37.48366783633823	40.0	36.5	41.0	33.0	41.0
50-51	37.26949866826551	40.0	36.0	41.0	32.0	41.0
52-53	37.081839599980015	39.0	35.5	41.0	32.0	41.0
54-55	36.852342033486174	39.0	35.0	41.0	31.0	41.0
56-57	36.590607972983534	39.0	35.0	41.0	31.0	41.0
58-59	36.32279347802414	38.0	35.0	40.0	31.0	41.0
60-61	36.05811858394155	37.5	35.0	40.0	30.5	41.0
62-63	35.748759225736364	37.0	35.0	40.0	30.0	41.0
64-65	35.44719535052353	36.5	35.0	40.0	30.0	41.0
66-67	35.13507259522318	36.0	34.0	39.0	29.0	41.0
68-69	34.83831346941494	35.5	34.0	39.0	29.0	41.0
70-71	34.55493017201731	35.0	34.0	39.0	29.0	40.5
72-73	34.27509842280104	35.0	34.0	37.5	29.0	40.0
74-75	33.950783474962236	35.0	34.0	37.0	28.5	39.5
76-77	32.44849960477144	34.0	31.5	35.5	25.5	38.5
78-79	33.35170499166625	35.0	33.0	36.0	27.5	39.0
80-81	33.35763963354562	35.0	33.0	36.0	28.0	38.5
82-83	33.15080442143535	35.0	33.0	35.5	28.0	37.0
84-85	32.95367210250063	35.0	33.0	35.0	27.0	37.0
86-87	32.77015992343968	35.0	33.0	35.0	27.0	36.5
88-89	32.609432510719856	35.0	33.0	35.0	27.0	36.0
90-91	32.45007078242443	35.0	33.0	35.0	27.0	36.0
92-93	32.29629631764846	35.0	33.0	35.0	26.0	36.0
94-95	32.177614369664894	35.0	33.0	35.0	25.5	35.0
96-97	32.06072959064058	35.0	32.5	35.0	25.0	35.0
98-99	31.96527849841332	35.0	32.0	35.0	25.0	35.0
100	30.0669779812212	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.045411754879857824
1101	2	-0.0681521174831019
1101	3	-0.040513313297985576
1101	4	-0.030854738057186637
1101	5	-0.020947029618028523
1101	6	-0.008029344272095784
1101	7	-0.01053158492140227
1101	8	-0.029968030020341985
1101	9	-0.08915084093949588
1101	10-11	-0.020150519173988357
1101	12-13	0.011866204274255665
1101	14-15	-0.030731179736143588
1101	16-17	-0.005535672891774368
1101	18-19	0.014373432241214346
1101	20-21	-0.023383779796709803
1101	22-23	-0.06726546620308937
1101	24-25	-0.06370997499050901
1101	26-27	-0.1101767191301164
1101	28-29	-0.07539959447199607
1101	30-31	-0.11971084760214978
1101	32-33	-0.13799264507655806
1101	34-35	-0.157462842668032
1101	36-37	-0.15914638042046647
1101	38-39	-0.09609343041783802
1101	40-41	-0.07008881072506767
1101	42-43	-0.1129714526652279
1101	44-45	-0.1108609840386876
1101	46-47	-0.13393545891103287
1101	48-49	-0.09126322820700494
1101	50-51	-0.05669046566415403
1101	52-53	-0.10536868643703201
1101	54-55	-0.10041677746920641
1101	56-57	-0.09707275058435272
1101	58-59	-0.09119835671950227
1101	60-61	-0.10425597612470483
1101	62-63	-0.10530897687767293
1101	64-65	-0.12383626630244038
1101	66-67	-0.21353393908765383
1101	68-69	-0.17027223858743668
1101	70-71	-0.15507495657412562
1101	72-73	-0.2001610836498955
1101	74-75	-0.14266299105610614
1101	76-77	-0.10702480650227386
1101	78-79	-0.13860780528627714
1101	80-81	-0.12540542485478312
1101	82-83	-0.09764976423102922
1101	84-85	-0.09354746843648343
1101	86-87	-0.09019124791108624
1101	88-89	-0.0943234960831063
1101	90-91	-0.15836659100868644
1101	92-93	-0.16687732102275277
1101	94-95	-0.14771907795969952
1101	96-97	-0.24109367259848824
1101	98-99	-0.20515622709480397
1101	100	-0.2115584667304269
1102	1	-0.018897046690433683
1102	2	-0.048625137735655244
1102	3	6.433872930955431E-4
1102	4	0.04868907523982102
1102	5	0.019170958789395343
1102	6	0.013530651810036431
1102	7	0.054623922048172346
1102	8	0.02648049359932969
1102	9	9.860520089262081E-4
1102	10-11	0.037992305774530166
1102	12-13	0.012373005758725242
1102	14-15	-0.0472728087389882
1102	16-17	0.003914159312266463
1102	18-19	0.040887258495402534
1102	20-21	-0.0014894783848617976
1102	22-23	0.0013720766797220563
1102	24-25	-8.193814418788747E-4
1102	26-27	-0.01656339315109534
1102	28-29	-0.04810027520148452
1102	30-31	-0.061709547318358204
1102	32-33	-0.01431061945603318
1102	34-35	-0.018308612325640183
1102	36-37	-0.07395989366028743
1102	38-39	-0.12794426176111529
1102	40-41	-0.04987013436665677
1102	42-43	-0.020141233289557192
1102	44-45	-0.027778639095352275
1102	46-47	-0.011397303280382687
1102	48-49	0.009241695274219808
1102	50-51	-0.031653880915776256
1102	52-53	-0.007654724800595147
1102	54-55	-0.039201082674409804
1102	56-57	0.010636598012055742
1102	58-59	-0.03833272090022888
1102	60-61	-0.07490397855178799
1102	62-63	-0.004267006721534017
1102	64-65	-0.02688170277529167
1102	66-67	-0.05263611450021699
1102	68-69	-0.0897524861435457
1102	70-71	-0.10384920149230936
1102	72-73	-0.1139647810543849
1102	74-75	-0.10152232093474822
1102	76-77	-0.12268512282027189
1102	78-79	-0.08291271374347531
1102	80-81	-0.06505003193499448
1102	82-83	-0.02633134577123286
1102	84-85	-0.05888461256004973
1102	86-87	-0.12143110565597226
1102	88-89	-0.06555663659427324
1102	90-91	-0.08036751320166502
1102	92-93	-0.10804622344709713
1102	94-95	-0.16244028889619244
1102	96-97	-0.1744092530944812
1102	98-99	-0.2439431158514367
1102	100	-0.1880493937958363
1103	1	-0.011021201271724124
1103	2	-0.01599044225366697
1103	3	0.01390022173320915
1103	4	-0.042714417391863435
1103	5	-0.08667406961890123
1103	6	-0.028469582370249213
1103	7	0.00917510437982827
1103	8	-0.061054842533010856
1103	9	-0.11276855493061788
1103	10-11	-0.08838322461593862
1103	12-13	-0.04248637455563653
1103	14-15	-0.023937649357002044
1103	16-17	-0.05018448355722427
1103	18-19	-0.026263729243581224
1103	20-21	-0.03873362248380374
1103	22-23	-0.0955453859211346
1103	24-25	-0.0675028449682955
1103	26-27	-0.07756331509099113
1103	28-29	-0.15135649631227466
1103	30-31	-0.1333839781766244
1103	32-33	-0.16447482444184658
1103	34-35	-0.13219125176048863
1103	36-37	-0.20974424428393235
1103	38-39	-0.14167503249304048
1103	40-41	-0.09060955861770026
1103	42-43	-0.013654927260525085
1103	44-45	0.004514125291400717
1103	46-47	-0.0791302569071135
1103	48-49	-0.08609546857949368
1103	50-51	-0.033388278654264525
1103	52-53	-0.017044936064664284
1103	54-55	0.00392119567673177
1103	56-57	0.01079369395348806
1103	58-59	-0.06631141049426503
1103	60-61	-0.13389796513660457
1103	62-63	-0.10132205024003582
1103	64-65	-0.060309753670473754
1103	66-67	-0.08226772660844972
1103	68-69	-0.18181509870341728
1103	70-71	-0.17858811835273514
1103	72-73	-0.09476687641036818
1103	74-75	-0.11474131016802858
1103	76-77	-0.1591015511248628
1103	78-79	-0.09018261767379698
1103	80-81	-0.14465850480075204
1103	82-83	-0.09694102299274476
1103	84-85	-0.1032681887160436
1103	86-87	-0.09990742835640276
1103	88-89	-0.13181955135822676
1103	90-91	-0.18421253045852382
1103	92-93	-0.16061537632779732
1103	94-95	-0.1311326150312695
1103	96-97	-0.14902438894692338
1103	98-99	-0.14553038424992337
1103	100	-0.06542548733413156
1104	1	0.005371536224274109
1104	2	-0.008064902279592445
1104	3	3.351302364364983E-4
1104	4	0.005528036438882111
1104	5	-0.007023306163738141
1104	6	-0.024198357980083074
1104	7	-0.009806562595478852
1104	8	0.011710094084214973
1104	9	0.02937189725032141
1104	10-11	-0.0022728476862496905
1104	12-13	-0.042268322670985015
1104	14-15	-0.11566076849640439
1104	16-17	-0.035991372330137494
1104	18-19	-0.056149842271977946
1104	20-21	-0.0717782681968373
1104	22-23	-0.06734194658509551
1104	24-25	-0.041038349421086195
1104	26-27	-0.07274643490379873
1104	28-29	-0.03120195345945831
1104	30-31	-0.08747860621319603
1104	32-33	-0.08013049522179472
1104	34-35	-0.057359220313202286
1104	36-37	-0.029205785335427947
1104	38-39	-0.03409931827947332
1104	40-41	-0.028028836070937757
1104	42-43	-0.07315312594405299
1104	44-45	-0.08775502842654959
1104	46-47	-0.04295043670206411
1104	48-49	-0.07951822240519135
1104	50-51	-0.018422831944612028
1104	52-53	-0.0998039805251949
1104	54-55	-0.031513224619217794
1104	56-57	-0.08830164955924147
1104	58-59	-0.10418093289287356
1104	60-61	-0.19706163241493613
1104	62-63	-0.16473891190902634
1104	64-65	-0.1674184302920807
1104	66-67	-0.14271186896295518
1104	68-69	-0.09636396993453644
1104	70-71	-0.06071455561810524
1104	72-73	-0.12435291031552254
1104	74-75	-0.05526697471913167
1104	76-77	-0.14842694126981115
1104	78-79	-0.08772537789668888
1104	80-81	0.008532266398383115
1104	82-83	-0.015168199302905805
1104	84-85	-0.03005704748946414
1104	86-87	-9.910380599507107E-4
1104	88-89	-0.03574343311105821
1104	90-91	-0.04817887782640895
1104	92-93	-0.07563389453456182
1104	94-95	-0.09763810315436672
1104	96-97	-0.08505202316183613
1104	98-99	-0.0972854893828341
1104	100	0.07941811799237897
1105	1	0.020761494076133147
1105	2	0.016965791240139083
1105	3	0.00919745578224962
1105	4	0.021401831261471216
1105	5	-0.0045096033362597154
1105	6	0.0015084800461835357
1105	7	-0.0014331848596924601
1105	8	0.026553371173903884
1105	9	0.0030729180346682483
1105	10-11	-0.001327773745920524
1105	12-13	0.0379153827037797
1105	14-15	0.06512599150004661
1105	16-17	0.03284145195383559
1105	18-19	-0.010656341094779975
1105	20-21	-0.029129486212383426
1105	22-23	0.02204398770155791
1105	24-25	-0.005906155284066017
1105	26-27	-0.027452020448905046
1105	28-29	0.049242331474715684
1105	30-31	0.008155939479586038
1105	32-33	-0.054685351699170326
1105	34-35	-0.027967670613954
1105	36-37	-0.034381356500198024
1105	38-39	0.004585275388464538
1105	40-41	-0.034681325855359546
1105	42-43	-0.005922525363466491
1105	44-45	0.027750737137296255
1105	46-47	-0.08999895142542869
1105	48-49	-0.06282927313026931
1105	50-51	-0.06406934090771443
1105	52-53	-0.13346254390734202
1105	54-55	-0.02306183333970324
1105	56-57	-0.09142015673842252
1105	58-59	-0.12444353888378856
1105	60-61	-0.11280724120297947
1105	62-63	-0.12840409985037837
1105	64-65	-0.09093512719245211
1105	66-67	-0.1233958424940198
1105	68-69	-0.12689771083288548
1105	70-71	-0.049962603041478815
1105	72-73	-0.01670293607317319
1105	74-75	-0.01844436234780744
1105	76-77	-0.10446820560029835
1105	78-79	-0.011373670770566946
1105	80-81	-0.03471891100714686
1105	82-83	0.06509468338949631
1105	84-85	0.06272097036755042
1105	86-87	0.04302088126366499
1105	88-89	0.053600320474870955
1105	90-91	0.045960657852212705
1105	92-93	0.009146172541136366
1105	94-95	0.0035346472828621245
1105	96-97	0.026940821663153258
1105	98-99	0.05298781844513201
1105	100	-0.03476127083913738
1106	1	0.011790717733390466
1106	2	0.0053360875595060975
1106	3	-0.019818086820798442
1106	4	0.056415016115074934
1106	5	0.033824898335140574
1106	6	0.04153744259006231
1106	7	0.0029346360404360894
1106	8	0.04157532479351289
1106	9	0.012021185690748837
1106	10-11	0.01422652529808488
1106	12-13	-0.0015500661151719441
1106	14-15	-0.006984442836220239
1106	16-17	-0.020215457987411867
1106	18-19	-0.003975598848832362
1106	20-21	0.026299156313804417
1106	22-23	0.007074772549970021
1106	24-25	0.023376281665669296
1106	26-27	0.013370921426663074
1106	28-29	0.012997099664730172
1106	30-31	0.015899022333030643
1106	32-33	0.05722681312763456
1106	34-35	0.04171912077449491
1106	36-37	-0.011874751927855698
1106	38-39	0.07707813656999463
1106	40-41	0.09290168001364663
1106	42-43	0.08115377227020559
1106	44-45	0.08848207607164937
1106	46-47	0.12331241772009349
1106	48-49	0.1906546046719484
1106	50-51	0.11096474468896389
1106	52-53	0.14865880311973
1106	54-55	0.14341413254084756
1106	56-57	0.04814040429477018
1106	58-59	0.09020406073277343
1106	60-61	0.048631108671486345
1106	62-63	0.06176018453916754
1106	64-65	0.001518143938035621
1106	66-67	-0.025895609599302816
1106	68-69	0.05331215814258172
1106	70-71	0.09444620606664955
1106	72-73	0.10538543438718762
1106	74-75	0.01693305469790829
1106	76-77	-0.012697813637160493
1106	78-79	0.09134800958695877
1106	80-81	0.1498880149236257
1106	82-83	0.0731054256010637
1106	84-85	0.10508415627639778
1106	86-87	0.19022298368541612
1106	88-89	0.09922394564286918
1106	90-91	0.11156203585704105
1106	92-93	0.11869732282966794
1106	94-95	0.0865026048503168
1106	96-97	0.09726429972497641
1106	98-99	0.09504541777997133
1106	100	0.14091268766405562
1107	1	0.017141591505193787
1107	2	0.02535694296936697
1107	3	0.033497835077028526
1107	4	0.03767284886650657
1107	5	0.011252003469159888
1107	6	0.030483336655422022
1107	7	0.006136693310118346
1107	8	0.013931812072399907
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2316	16-17	-0.12766955460457297
2316	18-19	-0.08570215191376462
2316	20-21	-0.09887826772924768
2316	22-23	-0.11990587730129221
2316	24-25	-0.09978367550787226
2316	26-27	-0.09536356913063315
2316	28-29	-0.1720487987336483
2316	30-31	-0.12513409809044163
2316	32-33	-0.07642468016458537
2316	34-35	-0.2043136057257655
2316	36-37	-0.1929427961818675
2316	38-39	-0.11851878669637017
2316	40-41	-0.04705302274379619
2316	42-43	-0.14690502940933214
2316	44-45	-0.1813414375675464
2316	46-47	-0.16789442767453266
2316	48-49	-0.09240997777035176
2316	50-51	-0.03753085681396584
2316	52-53	-0.14186887213093513
2316	54-55	-0.19234377451611095
2316	56-57	-0.29065006202633015
2316	58-59	-0.19474586268481175
2316	60-61	-0.10615625314801491
2316	62-63	-0.09501242642679841
2316	64-65	-0.10495636321840607
2316	66-67	-0.1351059723898942
2316	68-69	-0.09567830939896282
2316	70-71	-0.0928896806584305
2316	72-73	-0.10457569952924217
2316	74-75	-0.09466875279338893
2316	76-77	-0.09396852292671554
2316	78-79	-0.10253402605837181
2316	80-81	-0.1351338583878885
2316	82-83	-0.15297760495415247
2316	84-85	-0.1135143483016634
2316	86-87	-0.09931690040570373
2316	88-89	-0.14573986387011928
2316	90-91	-0.1632964523635394
2316	92-93	-0.19382179558709822
2316	94-95	-0.1569481875848382
2316	96-97	-0.1824490957608198
2316	98-99	-0.04633361646651579
2316	100	-0.22990428733311674
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	10.0
13	39.0
14	310.0
15	913.0
16	2613.0
17	6157.0
18	11855.0
19	18701.0
20	28232.0
21	42267.0
22	55755.0
23	55323.0
24	49377.0
25	47616.0
26	53188.0
27	64912.0
28	79734.0
29	98991.0
30	122643.0
31	153439.0
32	197288.0
33	264590.0
34	405917.0
35	619239.0
36	1098017.0
37	1962548.0
38	1855251.0
39	510563.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72982721099487	21.526814927711403	13.453971062011917	23.289386799281818
2	15.430091562668084	20.928183275533442	40.14297704070615	23.498748121092326
3	17.855049559139086	27.763853876287342	30.13822392170262	24.242872642870953
4	12.298504311445951	17.663950377567	39.60703616500258	30.430509145984463
5	12.784936836868477	38.80135147410535	34.99697057324134	13.41674111578483
6	29.193898693592953	38.85155888581119	18.09668357630611	13.857858844289753
7	26.003881094060095	32.94816201485844	23.724587090871847	17.32336980020962
8	26.117200047968957	34.82065709269181	20.24018302001474	18.8219598393245
9	26.457776381042038	14.869871901880483	21.62143083448455	37.05092088259293
10-11	25.270740247006295	26.1464843691198	27.827945880583922	20.754829503289983
12-13	25.645476913415227	24.203314426192104	28.16825397699819	21.982954683394475
14-15	23.19246191227916	25.210068907521254	25.85864721106845	25.73882196913113
16-17	21.756901386003854	27.856382963731452	27.05072482520896	23.335990825055735
18-19	21.34222514778303	27.536281644387806	29.487295611278718	21.634197596550447
20-21	22.82088186876733	26.681548727646557	28.769634394920207	21.7279350086659
22-23	23.231750079367256	26.294595877822342	28.473294295832446	22.00035974697796
24-25	22.601569583336698	26.656134364553452	28.957591629811297	21.784704422298553
26-27	22.458867506242754	26.791053734654785	28.366274218504078	22.38380454059838
28-29	21.74701524919375	27.06175558986998	28.897522584126534	22.293706576809733
30-31	22.548795908510314	26.61826898316181	28.680002613551782	22.1529324947761
32-33	22.868132215169265	27.0162469964186	27.96697086986459	22.14864991854754
34-35	22.764738483581944	26.934546076946255	28.67116252636585	21.629552913105954
36-37	22.625544028018325	26.56098190471255	28.406261908945556	22.407212158323564
38-39	22.64165168905099	26.528712886762307	28.87922381253205	21.950411611654648
40-41	22.284147186961924	25.87503568877886	29.448846104988974	22.391971019270244
42-43	22.260349307505166	26.20031775726558	28.695792323424165	22.84354061180509
44-45	22.306089409026306	26.94618760858365	28.46728911735384	22.28043386503621
46-47	22.408320458224274	26.691252525243463	27.89483046781951	23.005596548712752
48-49	23.051956018139634	26.484818926880482	27.95503004234712	22.50819501263277
50-51	22.704710229065338	26.574352946075237	27.551311884716217	23.16962494014321
52-53	22.09522325780338	27.067257698063944	27.58234851766935	23.255170526463328
54-55	22.229189009962095	27.03302902250744	27.36570737245152	23.37207459507895
56-57	22.974264443760728	26.58255660831388	27.193213235223933	23.249965712701457
58-59	22.08420417159339	26.9236904179114	27.838462956644776	23.153642453850427
60-61	22.2746718759429	27.38269743528902	27.234985395995494	23.10764529277259
62-63	22.559716625643595	26.738368639822564	27.013717745898845	23.688196988634996
64-65	21.81216441459043	26.821593796081938	27.351528879402565	24.014712909925066
66-67	22.445850261365702	26.499392019342636	27.260665028729665	23.794092690561996
68-69	21.914691153791335	26.550169192408525	27.52907338930909	24.006066264491054
70-71	21.99691969918543	26.18457475597203	27.59405932285525	24.224446221987286
72-73	22.41462732313865	26.38208008011124	27.612422137111714	23.590870459638396
74-75	22.415927451772994	26.772068983852048	27.3155272603576	23.496476304017353
76-77	22.163842244018714	26.70659902444274	27.03318553812754	24.096373193411
78-79	21.97255056654957	26.61096340011482	27.197230073973085	24.219255959362528
80-81	21.67383134441674	26.993197805320456	27.478398989830406	23.8545718604324
82-83	22.318369844475054	26.144375005364985	28.24271454456959	23.29454060559037
84-85	22.495345074330515	26.46476820049193	27.108287212196775	23.93159951298078
86-87	21.381279199143613	27.029178532734843	27.59284359582079	23.996698672300752
88-89	22.083771135322756	26.02936661403541	27.45099986042009	24.435862390221743
90-91	21.87872915922663	26.04741871477827	27.604366813625713	24.469485312369383
92-93	22.049113588264426	26.211125743768942	27.397126895890572	24.34263377207606
94-95	21.08858431997655	26.026068801917436	27.97896769707006	24.906379181035952
96-97	21.427498920628736	25.95041041730991	27.599555183736534	25.022535478324816
98-99	20.950492723143004	26.52753526712386	27.45470212730794	25.067269882425197
100	21.177313270992645	26.13102408615965	26.94527251770299	25.746390125144718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	692.0
1	479.5
2	352.0
3	459.0
4	424.5
5	445.5
6	724.0
7	1376.5
8	2308.5
9	3003.0
10	2956.0
11	2825.0
12	3164.5
13	3654.5
14	4361.0
15	5273.0
16	6186.5
17	7255.0
18	8466.0
19	9582.5
20	10814.0
21	11912.0
22	13106.0
23	14721.5
24	16673.0
25	19294.5
26	23117.5
27	27432.0
28	33761.0
29	46451.5
30	64400.5
31	82091.0
32	106260.0
33	132029.5
34	155522.0
35	179434.0
36	204202.0
37	243548.5
38	260588.0
39	249002.5
40	233576.5
41	216736.0
42	214964.5
43	226155.0
44	242543.5
45	281725.0
46	317977.0
47	339745.5
48	356297.0
49	351183.5
50	338139.5
51	336201.5
52	333123.0
53	321001.0
54	308902.0
55	275146.0
56	240951.5
57	199851.0
58	158905.0
59	144978.5
60	118128.5
61	84829.0
62	63475.0
63	49910.5
64	35386.0
65	19255.5
66	11542.5
67	8132.5
68	5803.0
69	4027.0
70	2889.5
71	2089.5
72	1443.5
73	934.5
74	680.5
75	452.5
76	257.0
77	137.5
78	62.5
79	30.0
80	16.0
81	6.0
82	3.0
83	3.5
84	3.0
85	2.0
86	2.0
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004714557862870423
2	0.03252788699409783
3	0.001114582973015562
4	0.0013836202423641458
5	0.006444083165825606
6	3.8433895621226274E-5
7	0.0010120925846922918
8	0.007199949779709722
9	0.0013964315409045545
10-11	2.4982032153797077E-4
12-13	7.046214197224816E-5
14-15	9.736586890710656E-4
16-17	5.1245194161635034E-5
18-19	0.0010120925846922918
20-21	1.9857512737633573E-4
22-23	0.0015886010190106859
24-25	0.0012683185555004669
26-27	0.0020882416620866275
28-29	2.1779207518694886E-4
30-31	0.001793581795657226
32-33	0.0017487422507657952
34-35	7.430553153437079E-4
36-37	0.0037216822259887442
38-39	9.352247934498393E-4
40-41	0.0036576257332867
42-43	0.003599974889854861
44-45	0.0035807579420442477
46-47	0.002235571595301328
48-49	0.009102427612960422
50-51	0.006693903487363576
52-53	0.004925944288787167
54-55	0.0041380494285520285
56-57	0.0030939285975087145
58-59	0.005009217729299824
60-61	0.007161515884088495
62-63	0.0031515794409405547
64-65	0.0028184856788899264
66-67	0.003369371516127503
68-69	0.0018896665347102916
70-71	0.0026070992529731818
72-73	0.005938036873479459
74-75	0.00762272263154321
76-77	0.004195700271983868
78-79	0.0026006936037029776
80-81	0.0024085241255968463
82-83	0.004701746564330014
84-85	0.0021587038040588756
86-87	0.0034013997624785255
88-89	5.829140835885984E-4
90-91	0.0018896665347102916
92-93	1.3451863467429196E-4
94-95	0.0024085241255968463
96-97	0.001793581795657226
98-99	0.0014220541379853721
100	8.96790897828613E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7805610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.14766463208227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4351991161278	36.37171011653734
2	17.155552036348265	18.235550524121475
3	6.352040528196763	10.12788359165989
4	3.02663434361386	6.4343418823332765
5	1.6214609593136347	4.308843163980773
6	0.9453029594900674	3.0144386800015774
7	0.5792111576725062	2.154860425139694
8	0.38801788922427866	1.6497795718192327
9	0.279869011573256	1.3386945931206986
>10	1.1448879996570234	10.163822926852738
>50	0.04634594275929521	1.677787723526724
>100	0.022783042553012837	2.2026661679179464
>500	0.0019391018283862822	0.7117147929863212
>1k	6.244487476567671E-4	0.52938341697471
>5k	3.286572355137002E-5	0.1342017616992747
>10k+	9.859717065411008E-5	0.9443206613284121
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22676	0.290509005702309	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19920	0.25520106692494243	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11466	0.1468943490643268	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
5	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
6	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
7	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
8	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
9	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
10-11	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
12-13	0.0	0.0	0.0	1.2811298540408757E-5	1.2811298540408757E-5
14-15	0.0	0.0	0.0	5.124519416163503E-5	1.2811298540408757E-5
16-17	0.0	0.0	0.0	5.124519416163503E-5	1.2811298540408757E-5
18-19	0.0	0.0	0.0	5.124519416163503E-5	1.2811298540408757E-5
20-21	0.0	0.0	0.0	6.40564927020438E-5	1.2811298540408757E-5
22-23	0.0	0.0	0.0	2.2419772445715327E-4	1.9216947810613137E-5
24-25	0.0	0.0	0.0	6.59781874831051E-4	2.5622597080817514E-5
26-27	0.0	0.0	0.0	0.0015757897204702772	2.5622597080817514E-5
28-29	0.0	0.0	0.0	0.005028434677110437	2.5622597080817514E-5
30-31	0.0	0.0	0.0	0.018832608854400874	2.5622597080817514E-5
32-33	0.0	0.0	0.0	0.052673653948890606	3.8433895621226274E-5
34-35	0.0	0.0	0.0	0.10569961860764246	3.8433895621226274E-5
36-37	0.0	0.0	0.0	0.19395024860324817	3.8433895621226274E-5
38-39	0.0	0.0	0.0	0.31852090996091276	3.8433895621226274E-5
40-41	0.0	0.0	0.0	0.45142532101911315	3.8433895621226274E-5
42-43	0.0	0.0	0.0	0.5852585512214932	3.8433895621226274E-5
44-45	0.0	0.0	0.0	0.7510879995285442	3.8433895621226274E-5
46-47	0.0	0.0	0.0	0.952737838554578	3.8433895621226274E-5
48-49	0.0	0.0	0.0	1.1745014162890537	3.8433895621226274E-5
50-51	0.0	0.0	0.0	1.404598743724065	3.8433895621226274E-5
52-53	0.0	0.0	0.0	1.6282071484483596	4.483954489143065E-5
54-55	0.0	0.0	0.0	1.8688545802313976	5.124519416163503E-5
56-57	0.0	0.0	0.0	2.1540020574945453	5.124519416163503E-5
58-59	0.0	0.0	0.0	2.4849178987933036	5.124519416163503E-5
60-61	0.0	0.0	0.0	2.8280493132503417	5.124519416163503E-5
62-63	0.0	0.0	0.0	3.173224129824575	5.124519416163503E-5
64-65	0.0	0.0	0.0	3.527014544667233	5.124519416163503E-5
66-67	0.0	0.0	0.0	3.91868796929388	5.7650843431839404E-5
68-69	0.0	0.0	0.0	4.350191721082657	6.405649270204378E-5
70-71	0.0	0.0	0.0	4.82481189810918	6.405649270204378E-5
72-73	0.0	0.0	0.0	5.3108341308366676	7.686779124245255E-5
74-75	0.0	0.0	0.0	5.77821592418786	7.686779124245255E-5
76-77	0.0	0.0	0.0	6.290776761841803	7.686779124245255E-5
78-79	0.0	0.0	0.0	6.843270417046201	7.686779124245255E-5
80-81	0.0	0.0	0.0	7.4377210749704386	7.686779124245255E-5
82-83	0.0	0.0	0.0	8.053079772112621	7.686779124245255E-5
84-85	0.0	0.0	0.0	8.640029414741448	7.686779124245255E-5
86-87	0.0	0.0	0.0	9.246682834525425	7.686779124245255E-5
88	0.0	0.0	0.0	9.717754281856255	7.686779124245255E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14970	0.0	52.01368	1
GTATCAA	26810	0.0	40.09207	1
CAACGCA	31125	0.0	33.343204	5
ATCAACG	31375	0.0	33.06381	3
TATCAAC	34135	0.0	31.379152	2
GTGGTAT	4310	0.0	30.65548	1
AACGCAG	35010	0.0	30.405487	6
TCAACGC	36590	0.0	28.607756	4
GTACTAG	6315	0.0	28.219215	1
GGGCACC	7075	0.0	27.900543	94
ACGCAGA	37330	0.0	27.747862	7
CGCAGAG	38035	0.0	27.147564	8
CTAACGC	3235	0.0	25.86307	3
TAGTACT	8740	0.0	25.76025	4
GTACATG	35925	0.0	25.666098	1
CTGTGCG	8520	0.0	25.593473	9
TAACGCC	3310	0.0	25.276562	4
TCTAACG	3350	0.0	25.264753	2
TAGGCAT	8095	0.0	25.141356	5
GCTATTG	8135	0.0	25.131357	8
>>END_MODULE