FastQCFastQC Report
Thu 26 May 2016
ERR580200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830944
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169660.2909648935060944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127830.2192269382110341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88050.151004708671529No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82900.049.5175321
GTATCAA146400.039.666621
CAACGCA169500.033.232585
ATCAACG171100.032.869393
TATCAAC184150.031.2811182
AACGCAG188700.030.5116656
GTACTAG33250.030.4050771
TCAACGC188300.030.0667614
GTACATG175750.029.9120221
GCATCGT29500.028.6787158
TACATGG179500.028.607252
ACATGGG176700.027.942373
ACGCAGA205350.027.5566277
CTGTGCG40200.027.4756079
CGCAGAG210800.026.7336228
TACCTGG38100.026.1654852
CATGGGG137000.025.5022644
TAGTACT45150.025.203924
CTAGTAC46000.024.6359713
GCAGAGT235850.023.4555729
Produced by FastQC (version 0.11.5)