FastQCFastQC Report
Thu 26 May 2016
ERR580200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830944
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148760.2551216406811659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118420.20308889949894907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.14044038152312902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79300.049.025041
GTATCAA143150.040.91781
CAACGCA174650.032.4808545
ATCAACG176250.032.266273
TATCAAC187950.030.8083422
TACCTGG38700.029.8761162
TCAACGC191650.029.7958814
AACGCAG195700.029.6828276
GTACTAG30800.027.0151981
GTACATG177350.026.6656651
GTACCTG46200.026.5573161
ACGCAGA215100.026.3720867
CGCAGAG219100.025.8477238
TACATGG179200.025.572112
ACATGGG177600.024.7435023
GTGGTAT32450.023.1787991
GCAGAGT245350.022.9290319
CTGTGCG40950.022.6094479
CATGGGG139400.022.5217674
TAGTACT44250.022.4108314
Produced by FastQC (version 0.11.5)