FastQCFastQC Report
Thu 26 May 2016
ERR580199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225560.31096609041343215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179730.24778300864517713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115960.15986712114001417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107950.051.6715771
GTATCAA195650.041.8031851
CAACGCA231850.033.932245
ATCAACG233350.033.7998623
TATCAAC248350.032.516352
AACGCAG256750.031.1392276
TCAACGC257900.030.7267934
GTACTAG41400.030.6727751
GTACATG248550.029.3107761
TACATGG252650.028.1653582
ACGCAGA280600.027.9233767
ACATGGG248050.027.4169223
CGCAGAG287500.027.2037938
GTGGTAT42650.026.6861551
GCATCGT39750.026.0135468
CTGTGCG55450.025.9387389
CATGGGG191300.025.3215894
TAGTACT59300.025.0611554
GCAGAGT319400.024.0167479
TACCTGG41900.023.6844922
Produced by FastQC (version 0.11.5)