FastQCFastQC Report
Thu 26 May 2016
ERR580199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199410.27491464838332375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164990.22746185164617916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111900.15426984180379083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107600.050.723521
GTATCAA188500.039.8524361
ATCAACG224000.032.4398653
CAACGCA224000.032.334955
TATCAAC243050.030.7300912
AACGCAG246450.029.9786036
TCAACGC248800.029.338554
GTACATG237600.028.5502131
TACATGG237850.028.2202342
ACATGGG237050.026.985833
GTACTAG41050.026.568331
ACGCAGA274000.026.4665327
CGCAGAG282750.025.5976318
CATGGGG183650.024.9790824
CTAACGC17500.024.978283
GTGGTAT41250.024.1602461
ATGGGGG136500.023.724895
TCTAACG19350.023.0769392
GCAGAGT316550.022.6120179
TAACGCC19650.022.4844844
Produced by FastQC (version 0.11.5)