FastQCFastQC Report
Thu 26 May 2016
ERR580198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5956961
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225660.3788173197709369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191790.32195946893055033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110260.18509437949988258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113350.054.3051221
GTATCAA212200.041.2847981
CAACGCA246600.034.4049265
ATCAACG247350.034.375173
TATCAAC263050.033.0985152
AACGCAG273900.031.6639086
GTACTAG41450.031.6520861
GTGGTAT37200.030.7175121
TCAACGC282650.030.2156184
ACGCAGA293350.028.8930597
CGCAGAG295350.028.6328128
GCATCGT40750.027.6701898
GTACATG277900.027.1079461
TAGGCAT51050.026.3333915
TACATGG284900.026.1542742
GCAGAGT316900.026.0362369
ACATGGG276450.025.9961593
TGGTATC44050.025.5069082
CTAGGCA57400.025.466784
CATGGGG215650.024.8691944
Produced by FastQC (version 0.11.5)