FastQCFastQC Report
Thu 26 May 2016
ERR580198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5956961
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196650.330117991371775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176140.29568768370314996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106420.17864813954632236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117850.054.3385771
GTATCAA212100.040.3008271
CAACGCA246950.033.5925755
ATCAACG247400.033.49463
TATCAAC267500.031.8313752
GTGGTAT36950.031.684411
GTACTAG44750.031.4151331
AACGCAG273550.030.7532546
TCAACGC278800.029.9404144
ACGCAGA296050.027.971497
CGCAGAG299550.027.4875438
GTACATG276800.027.2457661
TAGGCAT55550.026.8214845
TACATGG279750.026.504672
ACATGGG276850.025.90793
CTGTGCG52950.025.8273549
GCATCGT48400.025.4405848
GCAGAGT320550.025.348499
GGGCACC50000.025.00162194
TGGTATC45700.025.0006222
Produced by FastQC (version 0.11.5)