FastQCFastQC Report
Thu 26 May 2016
ERR580197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115618
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139710.19634274914701716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115680.1625719649368474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.043.403091
GTATCAA159100.037.5991251
CAACGCA169500.033.845355
ATCAACG175650.032.5308233
GTACTAG53150.032.1191561
GTACATG259850.030.8938181
AACGCAG195650.030.2439066
TATCAAC196400.030.219752
CTGTGCG71350.030.104799
TACATGG264350.029.8350492
ACATGGG256500.029.5009213
GCATCGT52350.029.2684028
CTAACGC24100.029.0761013
TAGTACT74200.029.0275344
TCAACGC201100.028.8102634
TCTAACG24800.028.2557982
TAACGCC25000.027.8400674
TAGGCAT67400.027.6276635
GGGCACC68550.027.42390494
CATGGGG204450.027.3721184
Produced by FastQC (version 0.11.5)