FastQCFastQC Report
Thu 26 May 2016
ERR580197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115618
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127130.17866332903199694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106070.1490664619713987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93900.043.504151
GTATCAA160500.038.397611
CAACGCA176450.033.376115
ATCAACG184300.032.0307853
AACGCAG201300.030.374946
TATCAAC202300.030.2275542
GTACATG253800.029.375691
GGGCACC63950.029.32978694
TAGGCAT66550.028.7443645
TACATGG257550.028.4880642
CTGTGCG71700.028.3169529
ACATGGG248050.028.2515133
TCAACGC210750.028.2115044
CTAACGC20950.028.0433123
TAGTACT76650.027.164074
CTAGGCA76000.026.9016534
TAACGCC22200.026.6760084
CATGGGG196650.026.6253014
ACGCAGA221750.026.5352157
TCTAACG22250.026.4059372
Produced by FastQC (version 0.11.5)