FastQCFastQC Report
Thu 26 May 2016
ERR580196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551435
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176130.18440161085742612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135630.14199960529491118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148800.043.573651
GTATCAA262050.040.2984351
CAACGCA275700.036.1436425
GTACTAG81100.035.0749551
ATCAACG286300.034.8523373
TATCAAC316500.033.1667142
AACGCAG324500.031.6931866
GCATCGT85500.030.5538758
TAGGCAT104450.029.6558115
ACGCAGA333450.029.5050757
TCAACGC347000.029.0287154
CGCAGAG338250.028.8784778
GGGCACC87450.028.167694
GTGGTAT42700.027.6381151
CTGTGCG105900.027.3771219
TAGTACT104800.027.3592154
CTAGGCA117700.027.235984
GTACATG382300.026.0362931
GCAGAGT370900.025.756049
TACATGG389100.025.2264842
Produced by FastQC (version 0.11.5)