FastQCFastQC Report
Thu 26 May 2016
ERR580196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551435
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152410.1595676461180964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126210.1321372128900003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148900.041.1689381
GTATCAA253100.038.280061
GTACTAG84050.033.7820471
CAACGCA272800.033.4228945
ATCAACG282350.032.3095743
TATCAAC313200.030.9356272
GGGCACC88650.030.16427694
AACGCAG324550.029.0334746
TAGGCAT109300.028.895875
GTGGTAT42900.027.6140421
CTAGGCA118850.027.1276134
CTGTGCG110200.026.9941839
TAGTACT111000.026.8866964
TCAACGC347050.026.7055514
ACGCAGA339350.026.659327
CGCAGAG343350.026.2255558
GCATCGT106450.025.0766128
CGACGAG53650.024.44022824-25
GTACATG378550.024.4020351
CTAACGC41650.024.1486193
Produced by FastQC (version 0.11.5)