FastQCFastQC Report
Thu 26 May 2016
ERR580195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8317681
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205110.24659517478489495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160580.1930586181412824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93070.11189416857895848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128650.052.3791281
GTATCAA239850.040.9366841
CAACGCA273000.034.2469145
ATCAACG276900.033.728993
TATCAAC298700.032.4236872
AACGCAG303900.031.3998326
TCAACGC314200.030.1149184
ACGCAGA325300.028.5270797
CGCAGAG329400.028.1287048
GTACTAG57300.027.4102951
GTACATG339100.027.0691071
TACATGG342900.026.283612
ACATGGG338250.025.7215443
GCAGAGT363250.025.096299
GCATCGT53750.025.0882178
CTGTGCG67250.024.5959599
CATGGGG259250.024.1325594
GGGCACC62700.023.76374694
TAGGCAT72150.023.4539245
TCTAACG25400.023.3220772
Produced by FastQC (version 0.11.5)