FastQCFastQC Report
Thu 26 May 2016
ERR580195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8317681
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178780.2149397169715934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147790.17768173605119023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86500.1039953323528517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129650.049.8247681
GTATCAA233600.040.2923241
CAACGCA265350.033.902465
ATCAACG268200.033.5599253
TATCAAC293050.031.6542572
AACGCAG298900.030.8348436
TCAACGC306400.029.574954
GTACTAG55050.029.5494821
ACGCAGA320900.028.032537
CGCAGAG325850.027.534578
TAGGCAT71950.026.5871625
GTACATG333000.026.1897141
TACATGG335200.025.6120012
ACATGGG331200.024.7211573
GCAGAGT361300.024.5708479
GTGGTAT46450.024.2916261
GCATCGT61750.024.1275048
CTAGGCA81150.024.0940914
CTGTGCG65950.023.7293369
CATGGGG255400.022.1938254
Produced by FastQC (version 0.11.5)