##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580195_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8317681
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.591562119297436	34.0	31.0	34.0	31.0	34.0
2	32.710036607559246	34.0	31.0	34.0	31.0	34.0
3	32.76423705116847	34.0	31.0	34.0	31.0	34.0
4	36.256711936896835	37.0	35.0	37.0	35.0	37.0
5	36.206372785876255	37.0	37.0	37.0	35.0	37.0
6	36.242329923448615	37.0	37.0	37.0	35.0	37.0
7	36.19533773896835	37.0	37.0	37.0	35.0	37.0
8	36.19002183421076	37.0	37.0	37.0	35.0	37.0
9	37.92149542642955	39.0	38.0	39.0	35.0	39.0
10-11	37.87659162451649	39.0	38.0	39.0	35.0	39.0
12-13	37.84102588209382	39.0	38.0	39.0	35.0	39.0
14-15	39.24364567479806	41.0	39.0	41.0	36.0	41.0
16-17	39.23502891010126	41.0	39.0	41.0	36.0	41.0
18-19	39.230480526964186	41.0	39.0	41.0	36.0	41.0
20-21	39.20442374503182	41.0	39.0	41.0	36.0	41.0
22-23	39.12936826983386	40.0	39.0	41.0	35.0	41.0
24-25	39.06251273642257	40.0	39.0	41.0	35.0	41.0
26-27	38.94365611039904	40.0	38.0	41.0	35.0	41.0
28-29	38.8500246042136	40.0	38.0	41.0	35.0	41.0
30-31	38.71159214930219	40.0	38.0	41.0	35.0	41.0
32-33	38.61666917738249	40.0	38.0	41.0	35.0	41.0
34-35	38.5292724618797	40.0	38.0	41.0	34.0	41.0
36-37	38.41573955529191	40.0	38.0	41.0	34.0	41.0
38-39	38.3079132873694	40.0	38.0	41.0	34.0	41.0
40-41	38.20738893448787	40.0	38.0	41.0	34.0	41.0
42-43	38.221227827804405	40.0	38.0	41.0	34.0	41.0
44-45	38.19530251280375	40.0	38.0	41.0	34.0	41.0
46-47	38.03113734465171	40.0	37.5	41.0	33.0	41.0
48-49	37.84988195628084	40.0	37.0	41.0	33.0	41.0
50-51	37.675848412556334	40.0	37.0	41.0	33.0	41.0
52-53	37.521061339091986	40.0	36.0	41.0	33.0	41.0
54-55	37.318091124196755	39.5	36.0	41.0	32.5	41.0
56-57	37.09206658682871	39.0	35.0	41.0	32.0	41.0
58-59	36.86020015675042	39.0	35.0	41.0	31.5	41.0
60-61	36.61455608840974	38.5	35.0	40.5	31.0	41.0
62-63	36.322702385436514	38.0	35.0	40.0	31.0	41.0
64-65	36.03683304276757	37.0	35.0	40.0	31.0	41.0
66-67	35.72719343288111	37.0	35.0	40.0	30.0	41.0
68-69	35.416611733486775	36.0	34.5	39.5	30.0	41.0
70-71	35.10485813293393	36.0	34.0	39.0	30.0	41.0
72-73	34.79319957089001	35.5	34.0	39.0	29.0	40.5
74-75	34.43972881383645	35.0	34.0	37.5	29.0	40.0
76-77	32.897997951592515	34.5	32.0	36.0	27.0	39.0
78-79	33.76636168181973	35.0	33.5	37.0	29.0	39.0
80-81	33.73012898667309	35.0	34.0	36.0	29.0	39.0
82-83	33.49184171645919	35.0	34.0	36.0	29.0	37.0
84-85	33.27068416064526	35.0	34.0	35.5	29.0	37.0
86-87	33.065499386187085	35.0	33.0	35.0	29.0	37.0
88-89	32.88444886260966	35.0	33.0	35.0	28.5	36.0
90-91	32.71650818298995	35.0	33.0	35.0	27.5	36.0
92-93	32.55936077615864	35.0	33.0	35.0	27.0	36.0
94-95	32.431268222476916	35.0	33.0	35.0	27.0	35.0
96-97	32.31549088021048	35.0	33.0	35.0	26.5	35.0
98-99	32.21790346371783	35.0	33.0	35.0	26.0	35.0
100	30.320330991294327	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	7.811193095506042E-4
1101	2	-0.009749007565616807
1101	3	0.004261790177373825
1101	4	0.017389390054439957
1101	5	-0.0391799323553812
1101	6	-0.024812315472587443
1101	7	0.009839330970528692
1101	8	0.010192644217141833
1101	9	-0.02758258828112048
1101	10-11	-0.006733006225950078
1101	12-13	0.02354188658785006
1101	14-15	-0.030526050598069787
1101	16-17	-0.027262724123843896
1101	18-19	-0.018054666275808984
1101	20-21	-0.03469966150813519
1101	22-23	-0.08261187700495753
1101	24-25	-0.08614213791728531
1101	26-27	-0.10203631002315916
1101	28-29	-0.07489946307666173
1101	30-31	-0.16463342735813313
1101	32-33	-0.1055074033909662
1101	34-35	-0.1439711790356739
1101	36-37	-0.1281939143417432
1101	38-39	-0.09392901897218309
1101	40-41	-0.10540056750297566
1101	42-43	-0.12823546893675797
1101	44-45	-0.1005383928084953
1101	46-47	-0.11750174396278368
1101	48-49	-0.10447810302242999
1101	50-51	-0.07762755098311658
1101	52-53	-0.04699713922344273
1101	54-55	-0.09778850556262597
1101	56-57	-0.151469932380067
1101	58-59	-0.1311264245946191
1101	60-61	-0.1098033011032058
1101	62-63	-0.15800382520281175
1101	64-65	-0.1315783225974485
1101	66-67	-0.14842299293942318
1101	68-69	-0.11255903679254686
1101	70-71	-0.1209654609289359
1101	72-73	-0.07309571685541272
1101	74-75	-0.09525990590960731
1101	76-77	-0.12015899510486605
1101	78-79	-0.09405508920436745
1101	80-81	-0.062031460724092824
1101	82-83	-0.08280380132340781
1101	84-85	-0.0820519097757213
1101	86-87	-0.10113197641325655
1101	88-89	-0.08506978851361424
1101	90-91	-0.11810719930912228
1101	92-93	-0.16641551054872394
1101	94-95	-0.1909071342246378
1101	96-97	-0.18651519142672868
1101	98-99	-0.2154090110824569
1101	100	-0.3354610438727299
1102	1	-0.005897348373821387
1102	2	-0.060123009018049345
1102	3	-0.014486394281590265
1102	4	-0.02491997813728375
1102	5	0.020969849779689298
1102	6	-0.008005852146510506
1102	7	0.027561734746853972
1102	8	0.00934115547349279
1102	9	-0.012591665986278144
1102	10-11	0.005987582009339576
1102	12-13	-0.029638033528343044
1102	14-15	-0.016303556044690026
1102	16-17	-0.051939557820283255
1102	18-19	-0.027820915368032217
1102	20-21	-0.04704579440574008
1102	22-23	-0.0294501122990809
1102	24-25	-0.05118979586935524
1102	26-27	-0.018391611402975627
1102	28-29	-0.06778118057847848
1102	30-31	-0.11173809329858386
1102	32-33	-0.13102800978167295
1102	34-35	-0.11269349203494983
1102	36-37	-0.12371860569977144
1102	38-39	-0.18397077641589732
1102	40-41	-0.21819786234684102
1102	42-43	-0.12045315230640341
1102	44-45	-0.1026453281752353
1102	46-47	-0.13083053844353287
1102	48-49	-0.09848772538989436
1102	50-51	-0.1265382263643815
1102	52-53	-0.14442451758220187
1102	54-55	-0.16000165007969258
1102	56-57	-0.13045050609103015
1102	58-59	-0.1557855567660127
1102	60-61	-0.19894137299862535
1102	62-63	-0.1910080917242425
1102	64-65	-0.2408666305132101
1102	66-67	-0.21534973585881545
1102	68-69	-0.31166488283466265
1102	70-71	-0.2963830353661123
1102	72-73	-0.31328771031946445
1102	74-75	-0.23388735689000129
1102	76-77	-0.1967734649668884
1102	78-79	-0.1970461930533105
1102	80-81	-0.21645729587296358
1102	82-83	-0.18076767205688782
1102	84-85	-0.20415884514245874
1102	86-87	-0.2615350265221892
1102	88-89	-0.20197202162908212
1102	90-91	-0.19946068514790483
1102	92-93	-0.19022678505852042
1102	94-95	-0.21753476675912964
1102	96-97	-0.30045219215294594
1102	98-99	-0.29810327397279934
1102	100	-0.20467128352414576
1103	1	0.001972639097054696
1103	2	-0.012515801722059905
1103	3	-0.02194018140850318
1103	4	-0.005774469030207285
1103	5	-0.01819091893128899
1103	6	-0.01776199763450137
1103	7	-0.05885157205008085
1103	8	-0.048821104883558064
1103	9	-0.10092277622144508
1103	10-11	-0.008000207374692536
1103	12-13	-0.012297677199143209
1103	14-15	-0.034049488478935075
1103	16-17	-0.017205848613258468
1103	18-19	0.00786772155017701
1103	20-21	-0.027027096038516163
1103	22-23	-0.07340582220060554
1103	24-25	-0.060153434723240196
1103	26-27	-0.06788934665837587
1103	28-29	-0.05638924690540392
1103	30-31	-0.08084865019933574
1103	32-33	-0.12521597918386362
1103	34-35	-0.12107906670875934
1103	36-37	-0.14162425365955755
1103	38-39	-0.14210095142475865
1103	40-41	-0.11119005129493331
1103	42-43	-0.03532443257631712
1103	44-45	-0.03704470746745159
1103	46-47	-0.10099678102049836
1103	48-49	-0.09475663052284489
1103	50-51	-0.1774282281710171
1103	52-53	-0.18952516703188138
1103	54-55	-0.11651617823149962
1103	56-57	-0.06614374024719893
1103	58-59	-0.09789212851401174
1103	60-61	-0.1610262944687122
1103	62-63	-0.16841906074867552
1103	64-65	-0.1360121231963447
1103	66-67	-0.13182595076143144
1103	68-69	-0.13451339013276709
1103	70-71	-0.1767055664367021
1103	72-73	-0.10128053862824515
1103	74-75	-0.10066435726775325
1103	76-77	-0.1756718127767556
1103	78-79	-0.17016833499372552
1103	80-81	-0.12196027769869744
1103	82-83	-0.08848166138601954
1103	84-85	-0.08119014976674066
1103	86-87	-0.0749213036128964
1103	88-89	-0.060930366693810356
1103	90-91	-0.11577785907982019
1103	92-93	-0.18675095787830287
1103	94-95	-0.1775258186423727
1103	96-97	-0.1310039692237126
1103	98-99	-0.08570695012077323
1103	100	0.023558983693426683
1104	1	0.057653647542203146
1104	2	0.016914200506441546
1104	3	0.025905374484381127
1104	4	0.03617361886303172
1104	5	-0.02354768622374337
1104	6	-0.005881171580014666
1104	7	0.017305083365229734
1104	8	0.03931506342888014
1104	9	-0.011431853705701656
1104	10-11	0.03782241399328967
1104	12-13	0.03429240161770508
1104	14-15	0.041004828636829416
1104	16-17	0.03690713798349066
1104	18-19	0.056821125065674494
1104	20-21	0.0659359680312761
1104	22-23	0.07391065649077433
1104	24-25	0.06906131766984203
1104	26-27	-0.023170127559737352
1104	28-29	0.04597826808527827
1104	30-31	0.012617591423541796
1104	32-33	0.03171895410741854
1104	34-35	0.024522227078520586
1104	36-37	0.036893452765518475
1104	38-39	0.045308736851040976
1104	40-41	0.021229859107151583
1104	42-43	0.0038502139853022754
1104	44-45	-0.058082414702901985
1104	46-47	0.0015118047056574824
1104	48-49	-0.0426288075231156
1104	50-51	-0.0414871170046851
1104	52-53	-0.08497077871759728
1104	54-55	-0.05491907502774751
1104	56-57	-0.07474025914206095
1104	58-59	-0.09728362523822653
1104	60-61	-0.09581697007058665
1104	62-63	-0.04965958977778939
1104	64-65	-0.019950602713542764
1104	66-67	-0.06425418344783651
1104	68-69	-0.07801256380717803
1104	70-71	-0.04154973785710325
1104	72-73	-0.057781736614515466
1104	74-75	-0.027836032584396264
1104	76-77	-0.09751211761430767
1104	78-79	0.041501518966946094
1104	80-81	0.04131277470094119
1104	82-83	0.00109313255727983
1104	84-85	0.017535615223899015
1104	86-87	0.0037852428766811386
1104	88-89	0.09454563080338829
1104	90-91	0.036129729458863835
1104	92-93	0.045687615720062524
1104	94-95	0.07344332667069153
1104	96-97	-0.042320818409777416
1104	98-99	-0.083083331769366
1104	100	0.048738892970327186
1105	1	0.042197174988835684
1105	2	0.02631005430335165
1105	3	0.03679827004078362
1105	4	0.022891087933082588
1105	5	0.042050359732456855
1105	6	0.01991070114304705
1105	7	-0.001111319992773474
1105	8	-0.005928094795699224
1105	9	-0.0074310019104189
1105	10-11	-0.019480011326180602
1105	12-13	0.008231648504121836
1105	14-15	0.008032817248547985
1105	16-17	0.00349254266787824
1105	18-19	-0.018531573222681175
1105	20-21	0.019621867944458415
1105	22-23	0.010805867034896721
1105	24-25	0.04796784859632908
1105	26-27	-0.05837208437736052
1105	28-29	-0.0332146168814802
1105	30-31	1.4264654438989055E-5
1105	32-33	-0.04449771105916511
1105	34-35	-0.0042363087524392995
1105	36-37	-0.011206744710555938
1105	38-39	0.04137089565798391
1105	40-41	-0.021684680659745936
1105	42-43	-0.05147366542210108
1105	44-45	0.04560036465770878
1105	46-47	0.07077852974784804
1105	48-49	-0.012247504891774952
1105	50-51	-0.04375904542383324
1105	52-53	-0.021527546553251398
1105	54-55	0.02279581655255214
1105	56-57	0.03639752033339505
1105	58-59	0.047656579340007
1105	60-61	0.033356136949485915
1105	62-63	0.01325165318309729
1105	64-65	0.05783999838433829
1105	66-67	-0.013266933072181075
1105	68-69	-0.014815443557118613
1105	70-71	0.09188324849293394
1105	72-73	0.04841622979649429
1105	74-75	0.06828000631907827
1105	76-77	0.017845356853143812
1105	78-79	0.08173494785756219
1105	80-81	0.10410540776975807
1105	82-83	0.1204905957502902
1105	84-85	0.14101188623869376
1105	86-87	0.09002521683740383
1105	88-89	0.10306080976346266
1105	90-91	0.12643771210685628
1105	92-93	0.11038478992967526
1105	94-95	0.08387804276036803
1105	96-97	0.07049805041590673
1105	98-99	0.08054461977133798
1105	100	0.044193230256695415
1106	1	-0.00650728934699174
1106	2	-0.018800548678889584
1106	3	-0.00467971240254883
1106	4	0.006093292383653193
1106	5	0.003680010175635573
1106	6	-0.029861464251922598
1106	7	0.00625595018776437
1106	8	-0.01375545714366666
1106	9	-0.011531969673001186
1106	10-11	-0.014857186895717689
1106	12-13	-0.03240478466926788
1106	14-15	-0.023885668707421814
1106	16-17	-0.02350397908396218
1106	18-19	-0.003045617780280452
1106	20-21	-0.0349005381087224
1106	22-23	-0.0044441973481852415
1106	24-25	-0.05260931836193805
1106	26-27	-0.03431224941148514
1106	28-29	-0.0530441242436126
1106	30-31	-0.07893914291757653
1106	32-33	-0.0464117674141491
1106	34-35	-0.036699013532071945
1106	36-37	-0.09033909193501444
1106	38-39	0.005785843071365093
1106	40-41	-0.06872326333669321
1106	42-43	-0.035297444510234754
1106	44-45	-0.008786405449903611
1106	46-47	-0.023186450239791156
1106	48-49	-0.0032773897361479953
1106	50-51	-0.06573551056944638
1106	52-53	0.0028466307770358412
1106	54-55	-0.015467853364718565
1106	56-57	-0.03383618016759726
1106	58-59	-0.04687363169923486
1106	60-61	-0.011397668083695578
1106	62-63	-0.004518168171692594
1106	64-65	-0.037050742341705245
1106	66-67	-0.02470922554313404
1106	68-69	-0.02958775362760946
1106	70-71	-0.029224420042979204
1106	72-73	-0.06438261154416836
1106	74-75	-0.09399605178018788
1106	76-77	-0.07026854810919048
1106	78-79	-0.003300223982812156
1106	80-81	-0.005310789987809983
1106	82-83	-0.07216956799581453
1106	84-85	0.016237439041567825
1106	86-87	0.03588850849781977
1106	88-89	-0.014747154474498814
1106	90-91	0.04941210046973765
1106	92-93	0.01527558705586074
1106	94-95	0.005104642061247944
1106	96-97	0.02544157883417597
1106	98-99	0.034149069224042705
1106	100	0.02576354861393071
1107	1	-0.002705007080869848
1107	2	0.03021381878237861
1107	3	0.014312625326247996
1107	4	0.02504119445056574
1107	5	0.009012220193206133
1107	6	-0.01024570848508688
1107	7	-0.03268991541816746
1107	8	-0.024353758135511328
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2316	12-13	-0.11905748424518237
2316	14-15	-0.20133001038426102
2316	16-17	-0.18985613235532384
2316	18-19	-0.13699051768327308
2316	20-21	-0.17639381379916586
2316	22-23	-0.18268648686940736
2316	24-25	-0.17006903839105547
2316	26-27	-0.21572017120142561
2316	28-29	-0.31943312154053416
2316	30-31	-0.21088299317105452
2316	32-33	-0.16615970356295406
2316	34-35	-0.25786626256132195
2316	36-37	-0.21934025964667825
2316	38-39	-0.17431205987085008
2316	40-41	-0.16367163805714569
2316	42-43	-0.2503108480330667
2316	44-45	-0.25191339825780545
2316	46-47	-0.24470691511335474
2316	48-49	-0.2730156624902378
2316	50-51	-0.20099001528134153
2316	52-53	-0.2433303009913601
2316	54-55	-0.31274206336541255
2316	56-57	-0.39457075237839945
2316	58-59	-0.4053886299978089
2316	60-61	-0.2665010393928142
2316	62-63	-0.2407092572172047
2316	64-65	-0.25783543307011314
2316	66-67	-0.33811522988533227
2316	68-69	-0.3138354061869606
2316	70-71	-0.321003676423544
2316	72-73	-0.29940161097142237
2316	74-75	-0.2721314174712859
2316	76-77	-0.30602241215369475
2316	78-79	-0.1962623064835327
2316	80-81	-0.2538489783974356
2316	82-83	-0.2950670445567738
2316	84-85	-0.25666475552575463
2316	86-87	-0.2673364711460593
2316	88-89	-0.3047781624531112
2316	90-91	-0.3187154268554977
2316	92-93	-0.28161503972820157
2316	94-95	-0.2582404028693688
2316	96-97	-0.3577683783950718
2316	98-99	-0.2831922452031641
2316	100	-0.5200967696999328
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	53.0
14	248.0
15	854.0
16	2254.0
17	5459.0
18	10138.0
19	16031.0
20	23755.0
21	35224.0
22	45958.0
23	47302.0
24	44493.0
25	45692.0
26	52574.0
27	64118.0
28	79511.0
29	98312.0
30	122143.0
31	154255.0
32	196633.0
33	262729.0
34	398921.0
35	600367.0
36	1050393.0
37	2002628.0
38	2311593.0
39	645775.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80129102318198	20.88785286630399	13.520575659419384	23.79028045109464
2	15.086295903384475	20.95342394055421	40.66314633532401	23.297133820737308
3	17.838195855949134	27.41876766292815	30.165113286802068	24.577923194320643
4	12.209766250042229	17.65839345010071	39.63390478677468	30.497935513082382
5	12.665438270064897	38.89369760402545	35.33293856313668	13.107925562772971
6	29.492605989315766	38.70502080027623	18.417135166809775	13.385238043598225
7	26.10257143334617	32.97122876740936	23.626490352541285	17.299709446703183
8	25.568883751961767	35.64123931826891	20.525528004221652	18.264348925547676
9	26.442797676678044	15.312675945254703	21.267706336932658	36.976820041134594
10-11	24.813852360012138	26.337309953275913	28.575794497934726	20.273043188777226
12-13	25.31873397764014	24.159485471477417	28.574975236463374	21.94680531441907
14-15	22.789217654987777	25.478540093612168	26.16953139995471	25.562710851445345
16-17	21.82175963287807	28.117246741801864	27.173899910101053	22.887093715219013
18-19	21.449891987969593	27.94275674979008	29.166921147370537	21.440430114869784
20-21	22.687630896189553	27.103903020801525	28.65570965872613	21.55275642428279
22-23	22.836652952484602	26.820460435112736	28.292000326298183	22.050886286104475
24-25	22.376278858167332	26.963216206260093	28.690961164647007	21.96954377092557
26-27	22.280518195483207	27.168851763762447	28.261804077959557	22.28882596279479
28-29	21.788919986039346	27.528610507208697	28.567267889374186	22.11520161737777
30-31	22.614120043847297	27.05786983532617	28.475044041165	21.852966079661535
32-33	22.621584422535758	27.32006308585433	27.97949373096947	22.07885876064045
34-35	22.62655299221982	27.323008856207238	28.47407233746463	21.576365814108314
36-37	22.4590835157184	27.076576238901424	28.30509423176657	22.15924601361361
38-39	22.48012107335744	27.07811097477374	28.578964225506343	21.86280372636248
40-41	22.321260194880615	26.52705967532802	28.92965228041124	22.222027849380126
42-43	22.181261667250688	26.76962732667965	28.386578347686758	22.6625326583829
44-45	22.221136809281113	27.26849133866523	28.353534237457602	22.15683761459606
46-47	22.194437980468688	27.000043102077566	27.89205171840529	22.91346719904846
48-49	22.60245230972043	26.909066052783647	28.106472199838063	22.382009437657857
50-51	22.475732877149618	26.940627383262733	27.81495679332153	22.768682946266118
52-53	22.07711831969633	27.267819077644397	27.754908663714133	22.90015393894515
54-55	21.97753900526102	27.17761126422222	27.788872781245615	23.05597694927114
56-57	22.572550006907203	26.896995828384373	27.481607535815577	23.048846628892846
58-59	21.966129071236654	27.146507228796736	28.051244520255025	22.836119179711588
60-61	22.282718573654385	27.501642563412503	27.508688347361478	22.706950515571634
62-63	22.34721915636177	27.023443853394642	27.440454978500505	23.188882011743086
64-65	21.733257740968547	26.993694016959708	27.69458456370808	23.578463678363665
66-67	22.159341787325264	26.908353569591842	27.48190008322941	23.45040455985349
68-69	21.775240454917096	26.934545538268008	27.729506485440275	23.56070752137462
70-71	21.826175429632023	26.59084603881905	27.73635509734819	23.846623434200737
72-73	22.037545557979062	26.812342787173826	27.682099959974266	23.468011694872846
74-75	22.08664967101842	27.022978512404244	27.487952130994824	23.40241968558251
76-77	21.84062370075368	26.8553447852566	27.318606878512096	23.98542463547762
78-79	21.758893729709104	26.805920701040858	27.41146607119649	24.023719498053552
80-81	21.608850898763485	27.131741657902996	27.522635152966345	23.73677229036717
82-83	22.05878984697384	26.56878518380386	28.049661768384425	23.32276320083787
84-85	22.126740876411752	26.750759181899	27.279805989920536	23.842693951768716
86-87	21.230274308439824	27.22769128629189	27.647396316319938	23.894638088948348
88-89	21.742516898721647	26.37809205759181	27.62352330426379	24.25586773942275
90-91	21.753122894881084	26.306491728366726	27.63903388063971	24.301351496112485
92-93	21.90243049421109	26.410892328567236	27.498480494027746	24.18819668319393
94-95	21.188578096789502	26.31354784428433	27.846131039725552	24.651743019200616
96-97	21.28871908672324	26.21314219468598	27.696468509703458	24.801670208887323
98-99	20.870270220879522	26.509383875042293	27.615224213429745	25.00512169064844
100	20.97797257575643	26.31544841185826	27.225293792966475	25.481285219418837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	633.0
1	444.0
2	327.5
3	414.0
4	381.0
5	395.5
6	622.5
7	1182.0
8	1944.5
9	2454.0
10	2452.5
11	2310.0
12	2593.5
13	3061.0
14	3543.5
15	4121.0
16	4798.0
17	5620.0
18	6570.0
19	7580.0
20	8676.5
21	9945.5
22	11562.5
23	13584.5
24	16203.5
25	19836.5
26	25074.5
27	31779.0
28	40205.0
29	53237.5
30	71871.0
31	92118.0
32	117131.0
33	144095.0
34	171738.5
35	198577.5
36	226477.0
37	263565.0
38	281890.5
39	280568.0
40	273604.0
41	264710.5
42	265561.5
43	275592.0
44	291544.0
45	322294.5
46	349365.5
47	362632.0
48	371672.5
49	366038.5
50	353653.0
51	348998.5
52	339495.5
53	320092.0
54	302848.5
55	269096.5
56	233565.0
57	195801.0
58	156966.0
59	136670.0
60	108790.5
61	78715.0
62	59109.5
63	45935.5
64	32851.0
65	19309.0
66	12395.5
67	8796.0
68	6490.0
69	4746.5
70	3507.0
71	2558.0
72	1764.0
73	1166.5
74	802.0
75	546.0
76	343.0
77	182.0
78	93.5
79	52.0
80	28.0
81	11.5
82	6.0
83	6.5
84	4.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004833077873508253
2	0.03298996439031504
3	0.001430687231212642
4	0.001418664649437746
5	0.007285684555587067
6	3.606774532468846E-5
7	0.0012263033410394074
8	0.006732645793941845
9	0.0012984388316887843
10-11	2.705080899351634E-4
12-13	9.618065419916922E-5
14-15	7.874791062556979E-4
16-17	1.021919450866173E-4
18-19	0.0010700097779657574
20-21	2.524742172728192E-4
22-23	0.0016290598304984287
24-25	0.001262371086364096
26-27	0.001965692120195521
28-29	2.4045163549792304E-4
30-31	0.002146030846818963
32-33	0.0015268678854118113
34-35	8.235468515803865E-4
36-37	0.004117734257901932
38-39	9.497839602167959E-4
40-41	0.003997508440152971
42-43	0.003961440694828282
44-45	0.003775090677317392
46-47	0.0026028889542650166
48-49	0.009461771856843272
50-51	0.006780736121041429
52-53	0.005079540799893624
54-55	0.004490434292923713
56-57	0.0033663228969709226
58-59	0.00553038761645223
60-61	0.007820689444569946
62-63	0.003330255151646234
64-65	0.0031559277159102397
66-67	0.003708966477555463
68-69	0.001989737283745313
70-71	0.002927498662187213
72-73	0.006396013504244753
74-75	0.007814678153682498
76-77	0.0043161068571877185
78-79	0.0026449679904771537
80-81	0.0023804711914294377
82-83	0.004538524620023297
84-85	0.0021039518106068266
86-87	0.0035105938782696764
88-89	4.8090327099584607E-4
90-91	0.0018995679204335918
92-93	1.262371086364096E-4
94-95	0.0022602453736804765
96-97	0.0019055792113210402
98-99	0.0013825969041130575
100	4.809032709958461E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8317681.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.77825585001931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05818713212285	41.056181163583446
2	16.86068544908287	19.483619953676158
3	5.841161770825976	10.124764177684053
4	2.5247353507013743	5.8349922018564895
5	1.2480490036962573	3.605504732446202
6	0.7121195677512329	2.468701594880154
7	0.4534273933354543	1.8338770759080254
8	0.2800362950851923	1.2944006963778996
9	0.1946997110066655	1.0124468744821147
>10	0.7634583584617449	7.323543192307809
>50	0.0373405895052525	1.4976379883364688
>100	0.0237191194497885	2.6075272010634047
>500	0.0017922081487372215	0.7225364392262287
>1k	5.040435659823355E-4	0.4659479499118907
>5k	2.8002420225640517E-5	0.13995103151875435
>10k+	5.6004840451281033E-5	0.5283677267408047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17878	0.2149397169715934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14779	0.17768173605119023	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8650	0.1039953323528517	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2022581774896152E-5	0.0
2	0.0	0.0	0.0	1.2022581774896152E-5	0.0
3	0.0	0.0	0.0	1.2022581774896152E-5	0.0
4	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	0.0
5	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	0.0
6	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	2.4045163549792305E-5
7	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	2.4045163549792305E-5
8	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	2.4045163549792305E-5
9	0.0	1.2022581774896152E-5	0.0	1.2022581774896152E-5	3.6067745324688454E-5
10-11	0.0	1.2022581774896152E-5	0.0	2.4045163549792305E-5	3.6067745324688454E-5
12-13	0.0	1.803387266234423E-5	0.0	2.4045163549792305E-5	3.6067745324688454E-5
14-15	0.0	2.4045163549792305E-5	0.0	6.011290887448076E-5	3.6067745324688454E-5
16-17	0.0	2.4045163549792305E-5	0.0	9.618065419916922E-5	3.6067745324688454E-5
18-19	0.0	2.4045163549792305E-5	0.0	1.0820323597406537E-4	3.6067745324688454E-5
20-21	0.0	2.4045163549792305E-5	0.0	1.4427098129875382E-4	3.6067745324688454E-5
22-23	6.011290887448076E-6	2.4045163549792305E-5	0.0	3.2460970792219613E-4	4.2079036212136535E-5
24-25	1.2022581774896152E-5	2.4045163549792305E-5	0.0	6.31185543182048E-4	4.809032709958461E-5
26-27	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.001430687231212642	4.809032709958461E-5
28-29	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.00474891980108398	4.809032709958461E-5
30-31	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.01837050495204132	5.4101617987032684E-5
32-33	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.05354256793450002	6.011290887448076E-5
34-35	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.11016892809426089	6.612419976192883E-5
36-37	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.2003623365695318	7.213549064937691E-5
38-39	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.3313122972617007	7.213549064937691E-5
40-41	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.4763046334669483	7.213549064937691E-5
42-43	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.6226314762492093	7.213549064937691E-5
44-45	1.2022581774896152E-5	2.4045163549792305E-5	0.0	0.7996820267572176	7.213549064937691E-5
46-47	1.2022581774896152E-5	2.4045163549792305E-5	0.0	1.0097105190737659	7.213549064937691E-5
48-49	1.2022581774896152E-5	2.4045163549792305E-5	0.0	1.2374783307991735	7.213549064937691E-5
50-51	1.2022581774896152E-5	2.4045163549792305E-5	0.0	1.4750806144164461	7.213549064937691E-5
52-53	1.2022581774896152E-5	2.4045163549792305E-5	0.0	1.7109756914216834	8.415807242427307E-5
54-55	1.2022581774896152E-5	2.4045163549792305E-5	0.0	1.9705191867781415	9.618065419916922E-5
56-57	1.2022581774896152E-5	2.4045163549792305E-5	0.0	2.2679879163435097	1.021919450866173E-4
58-59	1.2022581774896152E-5	2.4045163549792305E-5	0.0	2.6074936030848024	1.0820323597406537E-4
60-61	1.2022581774896152E-5	2.4045163549792305E-5	0.0	2.959021871600991	1.0820323597406537E-4
62-63	1.2022581774896152E-5	2.4045163549792305E-5	0.0	3.321346418551036	1.2022581774896152E-4
64-65	1.2022581774896152E-5	2.4045163549792305E-5	0.0	3.689652199934092	1.2022581774896152E-4
66-67	1.2022581774896152E-5	2.4045163549792305E-5	0.0	4.0933885298077675	1.2022581774896152E-4
68-69	1.2022581774896152E-5	2.4045163549792305E-5	0.0	4.540844978305852	1.2022581774896152E-4
70-71	1.2022581774896152E-5	2.4045163549792305E-5	0.0	5.027903811170445	1.2022581774896152E-4
72-73	1.2022581774896152E-5	2.4045163549792305E-5	0.0	5.5206733703781135	1.2022581774896152E-4
74-75	1.2022581774896152E-5	2.4045163549792305E-5	0.0	6.002346086607553	1.2022581774896152E-4
76-77	1.2022581774896152E-5	2.4045163549792305E-5	0.0	6.526446493920601	1.4427098129875382E-4
78-79	1.2022581774896152E-5	2.4045163549792305E-5	0.0	7.096395016832215	1.4427098129875382E-4
80-81	1.2022581774896152E-5	2.4045163549792305E-5	0.0	7.7040523674807915	1.6831614484854614E-4
82-83	1.2022581774896152E-5	2.4045163549792305E-5	0.0	8.327230871200758	2.043838901732346E-4
84-85	1.2022581774896152E-5	2.4045163549792305E-5	0.0	8.929351822941996	2.043838901732346E-4
86-87	1.2022581774896152E-5	2.4045163549792305E-5	0.0	9.551634644319734	2.043838901732346E-4
88	1.2022581774896152E-5	2.4045163549792305E-5	0.0	10.035850136594563	2.043838901732346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12965	0.0	49.824768	1
GTATCAA	23360	0.0	40.292324	1
CAACGCA	26535	0.0	33.90246	5
ATCAACG	26820	0.0	33.559925	3
TATCAAC	29305	0.0	31.654257	2
AACGCAG	29890	0.0	30.834843	6
TCAACGC	30640	0.0	29.57495	4
GTACTAG	5505	0.0	29.549482	1
ACGCAGA	32090	0.0	28.03253	7
CGCAGAG	32585	0.0	27.53457	8
TAGGCAT	7195	0.0	26.587162	5
GTACATG	33300	0.0	26.189714	1
TACATGG	33520	0.0	25.612001	2
ACATGGG	33120	0.0	24.721157	3
GCAGAGT	36130	0.0	24.570847	9
GTGGTAT	4645	0.0	24.291626	1
GCATCGT	6175	0.0	24.127504	8
CTAGGCA	8115	0.0	24.094091	4
CTGTGCG	6595	0.0	23.729336	9
CATGGGG	25540	0.0	22.193825	4
>>END_MODULE