FastQCFastQC Report
Thu 26 May 2016
ERR580194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085139
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389370.25811495671335877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316170.2095903789815924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194470.128914953982194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227350.043.626051
GTATCAA390500.038.1770551
CAACGCA431850.032.984325
ATCAACG443750.032.1980443
TATCAAC483650.030.3975012
AACGCAG489250.029.7348946
TCAACGC498100.029.014664
GTACATG502250.028.9429861
GTACTAG99300.028.937181
TACATGG504200.028.291092
ACATGGG502550.027.2609713
ACGCAGA523950.027.1824997
CTAACGC45200.026.7408263
TCTAACG45150.026.6662732
CGCAGAG539650.026.347438
TAACGCC46700.025.9821014
GCATCGT97300.025.7960668
CTGTGCG128050.025.5109969
CATGGGG410750.025.1664264
CGCCTAC49450.024.3338187
Produced by FastQC (version 0.11.5)