FastQCFastQC Report
Thu 26 May 2016
ERR580194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085139
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336950.22336552550162114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288490.19124119439668405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186310.12350565679242333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227750.044.2545551
GTATCAA387050.037.7854421
CAACGCA447000.031.3650955
ATCAACG456700.030.7299983
TATCAAC492250.029.4662912
GTACTAG100250.028.839141
AACGCAG500650.028.7727456
TCAACGC508200.027.8375664
GTACATG499150.027.284061
CTGTGCG130600.026.6660469
TACATGG502600.026.49352
GGGCACC122000.025.69957294
ACATGGG498500.025.6735673
ACGCAGA548900.025.5670227
TAGTACT147350.025.2942894
CGCAGAG565250.024.7526598
CTAACGC39700.024.7434083
TAGGCAT125300.024.3066315
CTAGTAC153800.024.1726283
TCTAACG41750.023.9794042
Produced by FastQC (version 0.11.5)