FastQCFastQC Report
Thu 26 May 2016
ERR580193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026138
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78360.1300335305962127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.10298469766208473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79450.044.8037831
GTATCAA139850.040.3825041
CAACGCA158750.034.088055
ATCAACG163100.033.2390333
TATCAAC173450.031.9633452
AACGCAG177100.031.0970256
TCAACGC177550.030.6922384
ACGCAGA197950.027.061737
CGCAGAG204850.026.0357278
GTACATG243350.024.5985621
GTGGTAT36400.023.6408141
TACATGG247700.023.5780722
ACATGGG251900.022.3615383
GTACTAG31850.022.2936021
GCAGAGT230700.022.1855099
CATGGGG190150.019.8062384
GAGTACT92700.019.8023612-13
TACCCCG5850.019.288395
TAGTACT43450.019.2617654
TGGTATC41050.019.2445962
Produced by FastQC (version 0.11.5)