FastQCFastQC Report
Thu 26 May 2016
ERR580193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026138
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68400.11350553206713819No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80550.044.9042741
GTATCAA139550.039.199991
CAACGCA161500.032.593195
ATCAACG166100.031.5776273
TATCAAC174800.030.9736522
AACGCAG181050.029.9288836
TCAACGC181000.029.2380494
GTGGTAT38250.026.260681
GTACTAG31200.025.9975051
ACGCAGA205000.025.561177
CGCAGAG211300.024.5768458
GTACATG246050.023.5551761
TACATGG249450.022.6088852
TGGTATC41900.022.0970132
ACATGGG252250.021.4637833
GCAGAGT238900.021.167019
CATGGGG191750.019.314324
TAGGCAT42650.018.953565
AGAGTAC214100.018.6464310-11
GAGTACT91350.018.46894312-13
Produced by FastQC (version 0.11.5)