##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580193_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6026138
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52276997307396	34.0	31.0	34.0	31.0	34.0
2	32.720992449890794	34.0	31.0	34.0	31.0	34.0
3	32.802782478595745	34.0	31.0	34.0	31.0	34.0
4	36.273398485066224	37.0	37.0	37.0	35.0	37.0
5	36.20489092682577	37.0	37.0	37.0	35.0	37.0
6	36.23104615261051	37.0	37.0	37.0	35.0	37.0
7	36.19896225410039	37.0	37.0	37.0	35.0	37.0
8	36.18916941497191	37.0	37.0	37.0	35.0	37.0
9	37.974456442915844	39.0	38.0	39.0	35.0	39.0
10-11	37.92566474581233	39.0	38.0	39.0	35.0	39.0
12-13	37.89012780656533	39.0	38.0	39.0	35.0	39.0
14-15	39.29266148568121	41.0	39.0	41.0	36.0	41.0
16-17	39.27635087347817	41.0	39.0	41.0	36.0	41.0
18-19	39.267744698179825	41.0	39.0	41.0	36.0	41.0
20-21	39.250015598713475	41.0	39.0	41.0	36.0	41.0
22-23	39.180255165082514	40.5	39.0	41.0	36.0	41.0
24-25	39.10237692863987	40.5	39.0	41.0	35.0	41.0
26-27	38.96661983180604	40.0	38.0	41.0	35.0	41.0
28-29	38.864234440034394	40.0	38.0	41.0	35.0	41.0
30-31	38.76636952223795	40.0	38.0	41.0	35.0	41.0
32-33	38.639639600022434	40.0	38.0	41.0	35.0	41.0
34-35	38.61989328820548	40.0	38.0	41.0	34.5	41.0
36-37	38.67895848053928	40.0	38.0	41.0	35.0	41.0
38-39	38.59494339492392	40.0	38.0	41.0	34.5	41.0
40-41	38.48252603906515	40.0	38.0	41.0	34.0	41.0
42-43	38.46818766845366	40.0	38.0	41.0	34.0	41.0
44-45	38.39682728805746	40.0	38.0	41.0	34.0	41.0
46-47	38.22858354720718	40.0	38.0	41.0	34.0	41.0
48-49	38.04149556482112	40.0	37.0	41.0	33.0	41.0
50-51	37.87807083077088	40.0	37.0	41.0	33.0	41.0
52-53	37.70427826246262	40.0	36.5	41.0	33.0	41.0
54-55	37.505400805623765	40.0	36.0	41.0	33.0	41.0
56-57	37.29072367741993	39.0	36.0	41.0	32.0	41.0
58-59	37.056085921032675	39.0	35.0	41.0	32.0	41.0
60-61	36.79155596834988	38.5	35.0	40.5	31.5	41.0
62-63	36.49829725107523	38.0	35.0	40.0	31.0	41.0
64-65	36.20336382937131	37.0	35.0	40.0	31.0	41.0
66-67	35.86803903262753	37.0	35.0	39.5	31.0	41.0
68-69	35.504680775647685	36.0	35.0	39.0	30.5	41.0
70-71	35.16651369417693	36.0	34.0	39.0	30.0	40.5
72-73	34.80311237479128	35.0	34.0	38.0	30.0	40.0
74-75	34.441682218362736	35.0	34.0	37.0	29.5	39.0
76-77	33.214817350681315	34.5	32.5	36.0	28.0	39.0
78-79	33.83637098918079	35.0	34.0	36.0	29.0	38.5
80-81	33.767862684193425	35.0	34.0	36.0	29.0	37.0
82-83	33.564177006898944	35.0	34.0	35.5	29.0	37.0
84-85	33.3552699921575	35.0	34.0	35.0	29.0	36.5
86-87	33.19361811827078	35.0	34.0	35.0	29.0	36.0
88-89	33.04328535124818	35.0	33.0	35.0	29.0	36.0
90-91	32.89790567358398	35.0	33.0	35.0	29.0	36.0
92-93	32.788769274782624	35.0	33.0	35.0	29.0	35.0
94-95	32.69559110660924	35.0	33.0	35.0	29.0	35.0
96-97	32.60510761618801	35.0	33.0	35.0	28.0	35.0
98-99	32.53699716136604	35.0	33.0	35.0	27.0	35.0
100	31.259211123276632	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.18339779284563917
1101	2	0.09886790677747115
1101	3	0.041450246355069
1101	4	0.052306282122557946
1101	5	0.0013375840264586714
1101	6	0.04269848957313371
1101	7	0.0064243763002949095
1101	8	0.048551171418232286
1101	9	0.023636221683098313
1101	10-11	0.032586433883956545
1101	12-13	-0.004732498620406034
1101	14-15	0.057920697616552275
1101	16-17	0.03714742297758278
1101	18-19	0.020737139324502607
1101	20-21	0.04410947215651362
1101	22-23	0.003898999795154623
1101	24-25	-0.0208423857365716
1101	26-27	-0.025740404380016457
1101	28-29	-0.10201807104771632
1101	30-31	-0.0954153813182188
1101	32-33	-0.06753639896538033
1101	34-35	-0.1919198672045468
1101	36-37	-0.04455344374929382
1101	38-39	-0.011590129614631905
1101	40-41	-0.056477014077344734
1101	42-43	-0.1492219704527855
1101	44-45	-0.22947320250739267
1101	46-47	-0.1542878575381721
1101	48-49	-0.09123361840921973
1101	50-51	-0.18299443081945554
1101	52-53	-0.20250331340322703
1101	54-55	-0.1972491643846297
1101	56-57	-0.12060063576007707
1101	58-59	-0.12116379798130339
1101	60-61	-0.20259266900174566
1101	62-63	-0.222156484978413
1101	64-65	-0.19273270392618258
1101	66-67	-0.28637730216589574
1101	68-69	-0.18500048829949378
1101	70-71	-0.2771190191722681
1101	72-73	-0.21538450093132866
1101	74-75	-0.24684104354768266
1101	76-77	-0.19197115213303562
1101	78-79	-0.16615483171336365
1101	80-81	-0.16092097877452716
1101	82-83	-0.13821016270683373
1101	84-85	-0.14391212342193427
1101	86-87	-0.29271186660327686
1101	88-89	-0.224348291157483
1101	90-91	-0.20804252751747754
1101	92-93	-0.2235107929266249
1101	94-95	-0.17435269474283643
1101	96-97	-0.18382697644667445
1101	98-99	-0.2987893710897609
1101	100	-0.2278945633556475
1102	1	0.04610395153164859
1102	2	-0.025014914533294075
1102	3	-0.018940363408269434
1102	4	-0.014551456724731793
1102	5	-0.02556458758235891
1102	6	-0.027539827748015
1102	7	-0.057503259351065594
1102	8	-0.010935095975412423
1102	9	-0.05033181895083061
1102	10-11	-0.03738424812501506
1102	12-13	-0.047713245837002205
1102	14-15	-0.021485162589840456
1102	16-17	-0.08685124997524696
1102	18-19	-0.10286023965133495
1102	20-21	-0.08859407907259964
1102	22-23	-0.09242830176253847
1102	24-25	-0.09094821032892497
1102	26-27	-0.16226377397748593
1102	28-29	-0.13850317674511103
1102	30-31	-0.1476028892677803
1102	32-33	-0.19341456077186336
1102	34-35	-0.15353099998558406
1102	36-37	-0.08715600372080701
1102	38-39	-0.14165188182566624
1102	40-41	-0.12228530451836406
1102	42-43	-0.12444900999285124
1102	44-45	-0.1483687855177962
1102	46-47	-0.10898816958505364
1102	48-49	-0.10643880194874811
1102	50-51	-0.12250408052988604
1102	52-53	-0.16420623714677163
1102	54-55	-0.17351325101155624
1102	56-57	-0.09315566793991792
1102	58-59	-0.13063978393919484
1102	60-61	-0.11597167876168868
1102	62-63	-0.10602395833147682
1102	64-65	-0.11050135678250683
1102	66-67	-0.15538258915574943
1102	68-69	-0.07183622275438495
1102	70-71	-0.18472770560756402
1102	72-73	-0.1343182418476232
1102	74-75	-0.15537103142194297
1102	76-77	-0.17425443642639493
1102	78-79	-0.13994289422449668
1102	80-81	-0.1990798386162993
1102	82-83	-0.10168403726058983
1102	84-85	-0.12028506607483536
1102	86-87	-0.1481687651286876
1102	88-89	-0.1882286534032005
1102	90-91	-0.15462410912028446
1102	92-93	-0.14965680203364684
1102	94-95	-0.1790533247722692
1102	96-97	-0.2570549656946284
1102	98-99	-0.2252378875951777
1102	100	-0.06850899043588754
1103	1	0.14179041888655775
1103	2	0.06611047113312196
1103	3	0.03292757772348409
1103	4	0.062414242284532406
1103	5	0.010356148195299397
1103	6	0.02595837292469838
1103	7	0.018893254796061854
1103	8	0.0264974899118684
1103	9	0.013233987252398549
1103	10-11	0.04639874178861447
1103	12-13	0.04153275407026058
1103	14-15	0.01745660434200147
1103	16-17	0.0032333755630133965
1103	18-19	0.06582186769145437
1103	20-21	0.02194355150938776
1103	22-23	-0.008125639491467496
1103	24-25	-0.01383478261313087
1103	26-27	-0.019100624545430378
1103	28-29	-0.031328783809129845
1103	30-31	-0.0639205284986133
1103	32-33	-0.05054705747462407
1103	34-35	-0.05220754812419415
1103	36-37	0.027665530188187404
1103	38-39	-0.01871892442611056
1103	40-41	0.02352743118164824
1103	42-43	0.026694684381403988
1103	44-45	0.051336552396627155
1103	46-47	0.08416129751858392
1103	48-49	0.04444624923798557
1103	50-51	0.025448665695414263
1103	52-53	-3.4839748262527337E-4
1103	54-55	-0.009390567301693409
1103	56-57	-0.025878971627754765
1103	58-59	-0.001726691932482538
1103	60-61	0.04050887858222296
1103	62-63	-0.019733826646813668
1103	64-65	-0.021196125199324456
1103	66-67	0.008629746238426605
1103	68-69	0.00572483952271341
1103	70-71	0.009514999756781606
1103	72-73	-0.06343566561211844
1103	74-75	-0.1374366936574276
1103	76-77	-0.0732460016416212
1103	78-79	-0.04546049873052738
1103	80-81	-0.02359118402998206
1103	82-83	0.0425191443727897
1103	84-85	0.018320439100996566
1103	86-87	0.06724643355219939
1103	88-89	0.07984718524938472
1103	90-91	0.09307570949113853
1103	92-93	0.06362247060029347
1103	94-95	0.01900333974836599
1103	96-97	0.003785927293662894
1103	98-99	0.03134654760370381
1103	100	0.13645485047273453
1104	1	0.11013220068695517
1104	2	0.0400797553619725
1104	3	0.03832249722930925
1104	4	0.028812466173569362
1104	5	0.04537302958669187
1104	6	0.08470755982101963
1104	7	0.034504446928600885
1104	8	0.002774669454169043
1104	9	-0.005710601181441177
1104	10-11	0.002949095473269381
1104	12-13	0.024926827573636956
1104	14-15	0.07428108386199028
1104	16-17	0.014643448986049634
1104	18-19	-0.029290120826381383
1104	20-21	0.02778614133218582
1104	22-23	-0.031193633012620126
1104	24-25	-0.010147849110566654
1104	26-27	-0.01887521518793278
1104	28-29	-0.039323562729016714
1104	30-31	-0.07763522927766076
1104	32-33	-0.08544047383957576
1104	34-35	-0.04978750607593696
1104	36-37	-0.010270115440249583
1104	38-39	-0.046627440428295586
1104	40-41	-0.042282098070671736
1104	42-43	-0.031052036641760594
1104	44-45	-0.05480814016549829
1104	46-47	-0.00232814542459181
1104	48-49	-0.03670123902187328
1104	50-51	-0.08101664537468167
1104	52-53	-0.08649123767082045
1104	54-55	-0.14406456822134572
1104	56-57	-0.03161491444901543
1104	58-59	-0.06392995043675853
1104	60-61	-0.11048115709669304
1104	62-63	-0.10223369134540405
1104	64-65	-0.15583011356683585
1104	66-67	-0.11158936154064492
1104	68-69	-0.0647203670731713
1104	70-71	-0.11648751593281048
1104	72-73	-0.10454936000373749
1104	74-75	-0.05834327606498846
1104	76-77	-0.18186545461264814
1104	78-79	-0.09856153927209022
1104	80-81	-0.11798242851838836
1104	82-83	-0.036960729114220214
1104	84-85	-0.02750879832591835
1104	86-87	-0.04039681676465534
1104	88-89	-0.031658312531078536
1104	90-91	0.0276469769875618
1104	92-93	-0.0203339864970431
1104	94-95	-0.055853649252888715
1104	96-97	-0.07512273114365087
1104	98-99	-0.08402000204618076
1104	100	-0.06327210769732972
1105	1	0.10528839344654983
1105	2	0.06315283056655119
1105	3	0.0437212794923596
1105	4	0.007005415207750332
1105	5	-0.001557368247169677
1105	6	-0.021930600714952675
1105	7	0.010289260449752646
1105	8	-0.03425258286981858
1105	9	-0.03319444323831533
1105	10-11	0.011340716689808517
1105	12-13	0.0010669518293298097
1105	14-15	0.0013106505646902633
1105	16-17	-0.00915904182227223
1105	18-19	-0.0429193572495663
1105	20-21	-0.04802544113207574
1105	22-23	-0.05249701186386346
1105	24-25	-0.12411259425474697
1105	26-27	-0.07496798089776036
1105	28-29	-0.09119005422258653
1105	30-31	-0.1497414531919361
1105	32-33	-0.07019240208972377
1105	34-35	-0.16344201432767136
1105	36-37	-0.07263060474754468
1105	38-39	-0.028057899710702827
1105	40-41	-0.021043148614147356
1105	42-43	0.0181288585616457
1105	44-45	0.018110936755370233
1105	46-47	-0.006880870818726237
1105	48-49	-0.07702969579857921
1105	50-51	-0.07487062917115139
1105	52-53	-0.13151121038216473
1105	54-55	-0.08570336650138444
1105	56-57	-0.06633602218975199
1105	58-59	-0.0341050756804222
1105	60-61	-0.04265897998039492
1105	62-63	-0.12696774832510016
1105	64-65	-0.07217549543758395
1105	66-67	-0.07930884461799081
1105	68-69	-0.02216894914126044
1105	70-71	0.01452294790884423
1105	72-73	-0.1002954292692948
1105	74-75	-0.14031758360071223
1105	76-77	-0.12344928740078132
1105	78-79	-0.08900927998852382
1105	80-81	-0.023685531397944715
1105	82-83	-0.0064188300505918505
1105	84-85	0.03374016361895116
1105	86-87	0.034935435063815135
1105	88-89	-0.03427501481704098
1105	90-91	0.0261348244407813
1105	92-93	-0.012318689087344126
1105	94-95	-0.04898702636077701
1105	96-97	-0.06546941662690386
1105	98-99	-0.07525539879637932
1105	100	-0.022084775008234914
1106	1	0.11148860882349965
1106	2	0.07246998922338577
1106	3	0.04096372015006722
1106	4	0.039592137913622594
1106	5	0.009985458289300198
1106	6	0.03864959932476353
1106	7	0.04309121992388043
1106	8	0.030226492121059323
1106	9	0.0016208319080917022
1106	10-11	0.06291895700737626
1106	12-13	0.003019583888708155
1106	14-15	0.02705359079060088
1106	16-17	0.0054577561242723505
1106	18-19	0.00976852242402515
1106	20-21	0.03681250073210407
1106	22-23	-0.012714253267148479
1106	24-25	-0.032630376386499904
1106	26-27	-0.060766788867795185
1106	28-29	-0.04095366041327253
1106	30-31	-0.02018514772244373
1106	32-33	-0.06642796924318617
1106	34-35	0.04698137882498088
1106	36-37	0.03598820980360529
1106	38-39	-0.016665501677096017
1106	40-41	-0.0042275334669739095
1106	42-43	-0.011415002858235823
1106	44-45	0.032754073169165565
1106	46-47	-0.06958869062608386
1106	48-49	-0.06380942970404391
1106	50-51	-0.08525977962052167
1106	52-53	-0.12879767429169675
1106	54-55	-0.08598878975839597
1106	56-57	-0.09743900225387847
1106	58-59	-0.13063913018632434
1106	60-61	-0.10362885184712667
1106	62-63	-0.1188196725455839
1106	64-65	-0.10696060400786678
1106	66-67	-0.08514210071518846
1106	68-69	-0.0455639107137884
1106	70-71	-0.1293940900709032
1106	72-73	-0.15280315867936167
1106	74-75	-0.11658136769062821
1106	76-77	-0.10799432232472839
1106	78-79	-0.05901684239061211
1106	80-81	-0.05647628960988982
1106	82-83	-0.015611615607426188
1106	84-85	-0.03289440624207174
1106	86-87	-0.06748765270280899
1106	88-89	-0.005985371258717009
1106	90-91	-0.03658317220524765
1106	92-93	-0.044182384472968295
1106	94-95	-0.01074609252219716
1106	96-97	-0.025140303325294155
1106	98-99	-0.06180552704611131
1106	100	0.05379373418814737
1107	1	0.06683122893199567
1107	2	-0.01682779631323683
1107	3	-0.027562057504546544
1107	4	-0.032593744877026154
1107	5	-0.025766561486392447
1107	6	-0.016734759338191907
1107	7	-0.004490785374208883
1107	8	-0.0020159026716086714
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2316	1	0.09802791735145178
2316	2	-0.01588901308976176
2316	3	-0.001506353370182012
2316	4	-0.09681894258183377
2316	5	-0.07242625511484846
2316	6	-0.03052855024452583
2316	7	-0.01845147773457967
2316	8	0.0035415861397041226
2316	9	0.02019961857294561
2316	10-11	-0.0036390186685224535
2316	12-13	0.04449931485576997
2316	14-15	0.04024106854784293
2316	16-17	0.010364399043652384
2316	18-19	1.4811723644925223E-4
2316	20-21	0.01570779560271518
2316	22-23	-0.015627050888198823
2316	24-25	0.008921456172764408
2316	26-27	0.05305278967679783
2316	28-29	0.037515152777238825
2316	30-31	-0.2908645093504916
2316	32-33	-0.10780094513960847
2316	34-35	-0.1959288091147542
2316	36-37	-0.48153463659450324
2316	38-39	-0.3020704829571059
2316	40-41	-0.2804074488393198
2316	42-43	-0.36250603358275413
2316	44-45	-0.17964611515876783
2316	46-47	-0.43005244562822753
2316	48-49	-0.36560959660982206
2316	50-51	-0.3469047202838027
2316	52-53	-0.37660596881008956
2316	54-55	-0.24600551996998377
2316	56-57	-0.9338259574244674
2316	58-59	-0.7681892278359683
2316	60-61	-0.4569367397714714
2316	62-63	-0.22843441732626957
2316	64-65	-0.18070785535697098
2316	66-67	-0.1068842621695012
2316	68-69	-0.21396896760138873
2316	70-71	-0.15582940970910641
2316	72-73	-0.006864556513292541
2316	74-75	-0.033601911410819696
2316	76-77	-0.03764544919283708
2316	78-79	-0.16141616895462363
2316	80-81	-0.16381381306705123
2316	82-83	-0.26495243734404283
2316	84-85	-0.14416697952808022
2316	86-87	-0.23268490009876786
2316	88-89	-0.2578128849417638
2316	90-91	-0.12706718983807974
2316	92-93	-0.0963829718138669
2316	94-95	-0.0727843806525641
2316	96-97	-0.2969727569074152
2316	98-99	-0.07715239413792574
2316	100	-0.2820212353421958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	13.0
13	46.0
14	189.0
15	580.0
16	1230.0
17	2588.0
18	4984.0
19	7841.0
20	11865.0
21	17395.0
22	23407.0
23	25821.0
24	26849.0
25	29845.0
26	36371.0
27	45682.0
28	56966.0
29	71331.0
30	88187.0
31	109535.0
32	139819.0
33	184481.0
34	271120.0
35	413841.0
36	746110.0
37	1558882.0
38	1839452.0
39	311666.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09914090846415	19.307660948937443	13.834008221220234	23.75918992137817
2	15.938980174099132	20.414025174129833	39.043747480753325	24.603247171017706
3	19.13030664242287	25.329135751373755	29.739019639698515	25.80153796650486
4	13.444905831145743	17.163718045541373	38.342346719743006	31.04902940356988
5	14.216092775007397	37.660016923761916	33.92859128351583	14.195299017714854
6	31.01078999518431	36.45364576782012	18.344966544078478	14.19059769291709
7	28.045936574603786	32.140683871171156	21.551857036060426	18.26152251816463
8	26.98562574975652	33.91749554398408	19.92682525730059	19.170053448958807
9	26.5604207133973	16.078049039529834	20.20239115625656	37.15913909081631
10-11	25.665217284883795	25.607186698931695	27.647533471924156	21.080062544260354
12-13	26.45703696598848	22.991448580466347	27.49723952371454	23.054274929830633
14-15	23.826157331234473	24.579825483266447	25.1615092081531	26.43250797734598
16-17	23.5139345626572	27.323327355478998	25.737185268624263	23.425552813239538
18-19	23.047292261753537	26.937158660827826	27.41344664965506	22.602102427763583
20-21	24.289748743159887	26.044350726573935	27.10615255293846	22.559747977327717
22-23	24.188284479946155	25.8291562439949	26.746486880626126	23.23607239543282
24-25	23.786212662239066	25.914831356334687	27.06605789645043	23.232898084975815
26-27	23.662056347230063	26.306716524971208	26.60988559512135	23.421341532677385
28-29	23.351133529184445	26.29923962269991	26.971873101556543	23.3777537465591
30-31	23.966724151550334	25.92652887553328	26.975569920138494	23.131177052777893
32-33	23.83620293696116	26.203118694566648	26.544619824093687	23.416058544378508
34-35	23.975409087876514	26.32208598222415	26.778614078418343	22.923890851480994
36-37	23.854538876321698	26.09170526721092	26.517229590117818	23.536526266349565
38-39	23.785157475283757	26.163476555044568	26.761512532798708	23.289853436872963
40-41	23.98420828795187	25.60767595041751	27.004956174472277	23.403159587158342
42-43	23.70484233323169	25.694291801245072	26.79257604328083	23.80828982224241
44-45	23.846123912112198	26.042157329914634	26.65363384873009	23.458084909243084
46-47	23.728568286129192	25.877952545850587	26.32341240256162	24.070066765458602
48-49	23.977285976214667	25.851259324661918	26.605198617010373	23.56625608211304
50-51	23.925744008650604	25.935472618536732	26.44233976519506	23.696443607617603
52-53	23.733663601628198	26.010379023028946	26.384030158279188	23.87192721706367
54-55	23.63311866871872	25.88813432298334	26.506405586347054	23.972341421950887
56-57	23.990278000304173	25.909446142559407	26.187767548523915	23.912508308612505
58-59	23.540269929275947	26.08749908937742	26.58768187767306	23.784549103673573
60-61	23.792297988388537	26.13901761566409	26.388033814162824	23.680650581784548
62-63	23.796142681537034	25.94444727934548	26.28040379500596	23.97900624411153
64-65	23.44604328127051	25.832015873571656	26.52814407685745	24.193796768300384
66-67	23.70947972953463	25.917672880015424	26.349057709036323	24.02378968141362
68-69	23.50108371389024	25.901052383183178	26.473393708004117	24.124470194922466
70-71	23.476858417054707	25.623308449036475	26.4214296850387	24.478403448870118
72-73	23.464585497058106	25.901612030792386	26.372166939748542	24.261635532400962
74-75	23.58867912942678	26.04001868667214	26.25219373399041	24.119108449910673
76-77	23.390342027271174	25.960570687677226	26.07251204504273	24.576575240008875
78-79	23.369946082362837	25.882589951648207	26.23125158582506	24.5162123801639
80-81	23.29620520818182	26.319538070509612	26.10571870149497	24.2785380198136
82-83	23.647502850502256	25.975372921697186	26.475984342192362	23.901139885608195
84-85	23.629037564328005	26.00690551911331	26.112795787688103	24.25126112887058
86-87	23.030344870275883	26.28485911230217	26.361354601607363	24.323441415814596
88-89	23.29441742507813	25.791294513674224	26.367519895984543	24.546768165263103
90-91	23.32950334435368	25.879441306165635	26.262415312521625	24.52864003695906
92-93	23.333125346517928	25.921532327374123	26.365100166394985	24.380242159712964
94-95	22.98858967538043	25.954555517092253	26.4701343158402	24.586720491687114
96-97	22.977873791014	25.964388441394416	26.433893940960377	24.623843826631205
98-99	22.768388063176477	25.995864426676462	26.332729202091237	24.90301830805582
100	22.995225504511552	25.84201698745282	26.101111902684	25.06164560535163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	52.0
2	74.0
3	115.5
4	91.0
5	71.5
6	157.5
7	333.5
8	569.0
9	743.5
10	715.5
11	682.0
12	751.5
13	895.5
14	1067.5
15	1293.5
16	1529.5
17	1754.0
18	2080.0
19	2434.5
20	2825.0
21	3267.0
22	3783.5
23	4541.0
24	5392.5
25	6329.5
26	7805.5
27	9696.0
28	12180.5
29	15669.5
30	20577.5
31	26832.5
32	35865.0
33	47488.5
34	60431.5
35	75713.5
36	96195.0
37	121034.0
38	142174.5
39	161759.5
40	178342.5
41	189460.0
42	202743.0
43	219930.5
44	237541.5
45	259893.5
46	279741.0
47	293957.5
48	305612.0
49	307572.5
50	300157.5
51	291112.5
52	280827.0
53	267809.0
54	254878.5
55	231825.0
56	205679.0
57	175434.5
58	144671.5
59	125360.5
60	103229.0
61	79083.0
62	60879.0
63	46717.0
64	33298.5
65	21916.5
66	15002.5
67	10695.5
68	7809.0
69	5687.5
70	4236.0
71	3175.0
72	2315.0
73	1591.5
74	1138.5
75	772.5
76	479.0
77	257.5
78	119.0
79	67.5
80	42.5
81	25.0
82	16.0
83	10.0
84	6.5
85	4.5
86	4.0
87	2.0
88	1.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3516348281436635
2	5.144256570294274E-4
3	0.0021406745082837467
4	0.001676031979353941
5	0.004828963425663335
6	0.0
7	4.978312809962201E-5
8	2.4891564049811006E-4
9	0.003036770814076943
10-11	9.126906818264036E-5
12-13	6.637750413282936E-5
14-15	1.8253813636528071E-4
16-17	1.1616063223245137E-4
18-19	9.458794338928182E-4
20-21	2.2402407644829908E-4
22-23	1.9913251239848804E-4
24-25	0.0
26-27	0.002115782944233936
28-29	0.010661886601335714
30-31	0.003418441462840712
32-33	0.01374844054351228
34-35	0.010952288181916842
36-37	0.012412593272839088
38-39	0.012412593272839088
40-41	0.013698657415412659
42-43	0.006231188200469356
44-45	0.0011367147582747026
46-47	0.0056835737913735135
48-49	0.013034882374084365
50-51	0.003318875206641467
52-53	0.005476144090958421
54-55	0.0050446903140950305
56-57	1.9083532438188438E-4
58-59	6.803694173615008E-4
60-61	3.982650247969761E-4
62-63	0.0010039597500090438
64-65	3.4848189669735405E-4
66-67	0.001327550082656587
68-69	0.001676031979353941
70-71	8.048272376105558E-4
72-73	0.002779557985562229
74-75	0.007517252343042924
76-77	0.0035677908471395776
78-79	0.0015764657231546971
80-81	3.8996783678037244E-4
82-83	1.1616063223245137E-4
84-85	0.0014686022789388495
86-87	0.004853854989713146
88-89	0.001833678551669411
90-91	1.8253813636528071E-4
92-93	0.0019083532438188438
94-95	0.0031861201983758086
96-97	0.0019415419958852586
98-99	0.0021489716963003502
100	0.0026716945413463814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6026138.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.32575097305144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3342606894277	46.95264371468209
2	14.627547890640358	18.233458143568697
3	4.76846847405049	8.915951359095525
4	2.016484553505819	5.0271565649123415
5	1.0178313025260664	3.1718550146908093
6	0.5669489326585821	2.1201310794789667
7	0.3921219174280834	1.7107505083688714
8	0.25722054877868494	1.282517109466556
9	0.18188522416695807	1.020251986839672
>10	0.7915541100005968	8.287077868281996
>50	0.03315268552356112	1.4166857631904832
>100	0.011864004967448162	1.288548014198909
>500	4.711915200630374E-4	0.18706980468145348
>1k	1.2564987048616164E-4	0.13614580380732028
>5k	6.282493523104989E-5	0.24975726473630078
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6840	0.11350553206713819	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6594376033207336E-5
2	0.0	0.0	0.0	0.0	1.6594376033207336E-5
3	0.0	0.0	0.0	0.0	1.6594376033207336E-5
4	0.0	0.0	0.0	0.0	3.318875206641467E-5
5	0.0	0.0	0.0	1.6594376033207336E-5	3.318875206641467E-5
6	0.0	0.0	0.0	1.6594376033207336E-5	6.637750413282935E-5
7	0.0	0.0	0.0	1.6594376033207336E-5	6.637750413282935E-5
8	0.0	0.0	0.0	1.6594376033207336E-5	6.637750413282935E-5
9	0.0	0.0	0.0	1.1616063223245137E-4	6.637750413282935E-5
10-11	0.0	0.0	0.0	1.9913251239848804E-4	6.637750413282935E-5
12-13	0.0	0.0	0.0	2.2402407644829905E-4	6.637750413282935E-5
14-15	0.0	0.0	0.0	2.8210439256452476E-4	7.467469214943302E-5
16-17	0.0	0.0	0.0	3.318875206641468E-4	1.1616063223245137E-4
18-19	0.0	0.0	0.0	4.480481528965981E-4	1.659437603320734E-4
20-21	0.0	0.0	0.0	5.476144090958421E-4	1.9913251239848806E-4
22-23	0.0	0.0	0.0	6.637750413282936E-4	1.9913251239848806E-4
24-25	0.0	0.0	0.0	8.877991177765926E-4	2.157268884316954E-4
26-27	0.0	0.0	0.0	0.001302658518606776	2.4891564049811006E-4
28-29	0.0	0.0	0.0	0.002538939533080723	2.4891564049811006E-4
30-31	0.0	0.0	0.0	0.006811991361631612	2.572128285147137E-4
32-33	0.0	0.0	0.0	0.017855548611731095	2.655100165313174E-4
34-35	0.0	0.0	0.0	0.037179699502401034	2.655100165313174E-4
36-37	0.0	0.0	0.0	0.07002826686013497	2.655100165313174E-4
38-39	0.0	0.0	0.0	0.12182761164779167	2.655100165313174E-4
40-41	0.0	0.0	0.0	0.18095337345410942	2.655100165313174E-4
42-43	0.0	0.0	0.0	0.24094204281415393	2.655100165313174E-4
44-45	0.0	0.0	0.0	0.32037932088511745	2.655100165313174E-4
46-47	0.0	0.0	0.0	0.4139384129603404	2.8210439256452476E-4
48-49	0.0	0.0	0.0	0.520507495845598	3.152931446309394E-4
50-51	0.0	0.0	0.0	0.631797678712303	3.152931446309394E-4
52-53	0.0	0.0	0.0	0.7441581988331498	3.318875206641468E-4
54-55	0.0	0.0	0.0	0.8732873359355529	3.484818966973541E-4
56-57	0.0	0.0	0.0	1.0235494109162453	3.484818966973541E-4
58-59	0.0	0.0	0.0	1.1953177972359743	3.484818966973541E-4
60-61	0.0	0.0	0.0	1.3781380379938195	3.484818966973541E-4
62-63	0.0	0.0	0.0	1.566650149731055	3.484818966973541E-4
64-65	0.0	0.0	0.0	1.7622314656584366	3.484818966973541E-4
66-67	0.0	0.0	0.0	1.9829034781480277	3.484818966973541E-4
68-69	0.0	0.0	0.0	2.231769335518038	3.484818966973541E-4
70-71	0.0	0.0	0.0	2.507368732677546	3.6507627273056143E-4
72-73	0.0	0.0	0.0	2.7907094062565445	4.480481528965981E-4
74-75	0.0	0.0	0.0	3.0755850596186147	4.480481528965981E-4
76-77	0.0	0.0	0.0	3.387451465598697	4.480481528965981E-4
78-79	0.0	0.0	0.0	3.731593932963367	4.480481528965981E-4
80-81	0.0	0.0	0.0	4.100237996541068	4.729397169464091E-4
82-83	0.0	0.0	0.0	4.480025183625068	5.144256570294275E-4
84-85	0.0	0.0	0.0	4.87014236978974	5.144256570294275E-4
86-87	0.0	0.0	0.0	5.277750028293411	5.144256570294275E-4
88	0.0	0.0	0.0	5.599606248645484	5.144256570294275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8055	0.0	44.904274	1
GTATCAA	13955	0.0	39.19999	1
CAACGCA	16150	0.0	32.59319	5
ATCAACG	16610	0.0	31.577627	3
TATCAAC	17480	0.0	30.973652	2
AACGCAG	18105	0.0	29.928883	6
TCAACGC	18100	0.0	29.238049	4
GTGGTAT	3825	0.0	26.26068	1
GTACTAG	3120	0.0	25.997505	1
ACGCAGA	20500	0.0	25.56117	7
CGCAGAG	21130	0.0	24.576845	8
GTACATG	24605	0.0	23.555176	1
TACATGG	24945	0.0	22.608885	2
TGGTATC	4190	0.0	22.097013	2
ACATGGG	25225	0.0	21.463783	3
GCAGAGT	23890	0.0	21.16701	9
CATGGGG	19175	0.0	19.31432	4
TAGGCAT	4265	0.0	18.95356	5
AGAGTAC	21410	0.0	18.64643	10-11
GAGTACT	9135	0.0	18.468943	12-13
>>END_MODULE