FastQCFastQC Report
Thu 26 May 2016
ERR580192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7154704
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149410.20882764681809338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117440.1641437577291807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115550.044.642191
GTATCAA205250.040.115461
CAACGCA224050.034.930755
ATCAACG233100.033.674463
TATCAAC255800.032.0333482
GTACTAG72050.031.3268011
GCATCGT68800.030.7976178
AACGCAG267200.030.6638646
GGGCACC64300.030.19101594
ACGCAGA271350.028.7932997
CGCAGAG273950.028.4514278
GTGGTAT35100.028.1333221
TAGGCAT87400.028.0197945
GTACATG316050.027.1380021
TCAACGC294450.027.090564
TATTCTC37800.026.8596635
TACATGG319400.026.4790082
CTGTGCG81550.026.4465719
CTAACGC38550.026.0925083
CTAGGCA96400.025.9894984
Produced by FastQC (version 0.11.5)