FastQCFastQC Report
Thu 26 May 2016
ERR580192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7154704
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131300.18351562831949442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106430.14875528044207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119900.044.232681
GTATCAA206800.038.286431
CAACGCA223050.033.5252885
GGGCACC67500.032.79331294
ATCAACG230100.032.5802733
GTGGTAT31000.032.0017781
GTACTAG68200.031.781171
TATCAAC261350.030.2043422
AACGCAG267550.029.2469436
TAGGCAT85900.028.5068425
CTGTGCG86450.028.1596249
TAGTACT90700.027.724084
ACGCAGA271750.027.2557327
CGCAGAG273850.026.9607268
AGGGCTA72500.026.2555795
TATTCTC35150.026.2080885
CTAGGCA95750.026.1636944
GCTATTG72400.026.09498
TCAACGC293550.025.8583664
CTATTGA75050.025.3610029
Produced by FastQC (version 0.11.5)