FastQCFastQC Report
Thu 26 May 2016
ERR580191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9358639
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277120.29611143244226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206580.2207372247182523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141550.15125062522445837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135000.049.0152321
GTATCAA248550.042.422141
CAACGCA289600.034.813355
ATCAACG297800.033.922463
TATCAAC314700.033.0725022
AACGCAG324600.031.6977736
TCAACGC328100.031.0472354
GTACTAG55400.030.3909281
GTACATG337400.028.9509071
ACGCAGA355250.028.3801987
TACATGG344950.027.8271792
CGCAGAG366950.027.5001958
ACATGGG342100.027.0138933
GCATCGT55450.026.0224048
CATGGGG272850.024.7514824
GCAGAGT400600.024.7092939
GTGGTAT50300.024.4031
TAGGCAT74400.024.3336585
CTGTGCG75500.023.0961659
TAGTACT76650.022.8245124
Produced by FastQC (version 0.11.5)