FastQCFastQC Report
Thu 26 May 2016
ERR580191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9358639
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250620.26779534930239324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190620.20368346294797782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134650.1438777582937006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.047.237561
GTATCAA241900.042.5775451
CAACGCA298600.033.5740135
ATCAACG302200.033.158863
TATCAAC322700.031.8248442
GTACTAG55250.031.395561
AACGCAG335850.030.5626456
TCAACGC332850.030.2463594
GTACATG339450.028.084471
TACATGG341950.027.6416052
ACGCAGA367750.027.2469657
CGCAGAG378500.026.4482758
ACATGGG345050.026.3439733
GTGGTAT50600.024.8976541
CTGTGCG79150.024.5235189
CATGGGG277050.024.4290494
TAGGCAT77150.023.880995
GCAGAGT416150.023.7505159
TAGTACT80850.023.6019674
CTAGGCA84500.022.9718274
Produced by FastQC (version 0.11.5)