FastQCFastQC Report
Thu 26 May 2016
ERR580190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736796
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118810.17635980071238613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101310.15038306043406985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.044.439461
GTATCAA153850.037.5381281
CAACGCA163750.033.16915
GTACTAG52750.032.363531
ATCAACG171950.031.5627883
TATCAAC190000.030.0994242
TAGGCAT63600.029.501825
AACGCAG191850.029.3990366
GTACATG238450.029.3676431
TACATGG240950.028.6925132
TAGTACT76700.027.9594274
GCATCGT55700.027.9301688
CTGTGCG75750.027.672799
ACATGGG240300.027.6541373
TCAACGC202700.027.0754644
CTAGTAC87650.026.5061593
GGGCACC69550.026.48968394
TCTAACG24250.026.3757822
ACGCAGA205500.026.3480137
CTAGGCA73000.026.2842834
Produced by FastQC (version 0.11.5)