FastQCFastQC Report
Thu 26 May 2016
ERR580190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736796
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103010.15290651520396345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89290.13254075082576344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.041.3611151
GTATCAA151000.040.035141
CAACGCA166350.034.695695
ATCAACG174100.032.9087453
TATCAAC191650.031.1221032
AACGCAG193150.030.707336
CTAACGC22650.030.0888963
GGGCACC69550.029.53602294
CTGTGCG77750.029.2565029
TAACGCC23500.029.200364
TAGTACT76600.029.0839124
TCTAACG23650.028.8172782
TCAACGC203550.028.6090474
CTAGTAC85050.027.7971383
GTACATG238750.027.742461
ACGCAGA208450.027.5515967
TAGGCAT70350.026.8572755
TACATGG245850.026.593352
GTACTGT89050.026.493986
CGCAGAG217300.026.3213568
Produced by FastQC (version 0.11.5)