FastQCFastQC Report
Thu 26 May 2016
ERR580189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6113353
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86230.1410518908363381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68660.11231152527917167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96100.044.9101561
GTATCAA176250.040.865321
CAACGCA190650.035.8508575
ATCAACG198800.034.3847733
TATCAAC215400.033.3071632
AACGCAG230700.030.8785216
GTGGTAT29350.028.8329851
ACGCAGA235300.028.8165657
CGCAGAG241050.028.0122158
TCAACGC246200.027.9930254
GTACTAG53750.027.9021361
GCATCGT51600.026.2265178
GCAGAGT265350.024.9197629
TAGGCAT72100.024.3189895
CGTTAAC15700.024.2555221
GTACATG301300.023.5146981
CTAGGCA78200.023.1450374
TACATGG305500.022.7299292
GGGCACC53550.022.3778194
ACATGGG300450.022.344683
Produced by FastQC (version 0.11.5)