FastQCFastQC Report
Thu 26 May 2016
ERR580189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6113353
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75540.12356557849677584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.10529409965365978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.045.0614281
GTATCAA173550.039.16251
CAACGCA186050.034.303255
ATCAACG191050.033.5047153
TATCAAC212050.031.870012
AACGCAG226050.029.3135956
TACCCCG5500.028.1978785
TCAACGC240450.027.0507954
GTACTAG51150.026.9257741
GTATAAG47700.026.7967341
ACGCAGA236500.026.7267197
CGTTAAC19200.026.5309941
CGCAGAG239200.026.346458
GTGGTAT30650.026.0068171
GTGATCG35350.025.5250348
GGGCACC54450.024.85885294
TAGGCAT67750.023.5849425
GCAGAGT264150.023.4485369
TGATCGC39150.023.2873959
GTACATG303400.022.9612711
Produced by FastQC (version 0.11.5)