FastQCFastQC Report
Thu 26 May 2016
ERR580188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7567099
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219500.29007153203625324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177810.23497776360531294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106010.1400933171351399No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130000.049.4898761
GTATCAA235900.040.989141
CAACGCA266950.034.5126655
ATCAACG270950.034.0531773
TATCAAC294200.032.6155362
GTACTAG61050.031.9695151
AACGCAG299700.031.588646
TCAACGC314200.029.6215954
ACGCAGA317400.028.851267
GTGGTAT43550.028.6174771
CGCAGAG321150.028.3965768
TAGGCAT74400.028.2414445
GCATCGT64200.027.1510878
CTGTGCG72700.026.499179
CTAGGCA81650.026.3093134
TAGTACT75850.026.1520844
GTACATG299300.025.8326361
GCAGAGT347900.025.7020159
GGGCACC64800.025.67829794
TACATGG305050.024.9825132
Produced by FastQC (version 0.11.5)