FastQCFastQC Report
Thu 26 May 2016
ERR580188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7567099
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191690.2533203279090177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163390.2159215836874871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97610.12899262980436757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131200.049.3846741
GTATCAA232750.040.3427161
CAACGCA270200.033.0688065
ATCAACG274050.032.7073633
TATCAAC296900.031.3572182
GGGCACC64400.030.2861394
AACGCAG305650.029.9991346
GTGGTAT41700.029.091121
TCAACGC317700.028.494494
ACGCAGA323000.027.4856057
CGCAGAG328200.026.8926038
CTGTGCG80450.026.8128139
TAGTACT83200.026.7214244
TAGGCAT72700.025.0852895
GTACATG297450.024.8651751
GCAGAGT355900.024.5474959
TACATGG301350.024.3252582
ACATGGG292100.024.2657893
TACCCCG6050.024.0839715
CTAGGCA80450.023.8372344
Produced by FastQC (version 0.11.5)