FastQCFastQC Report
Thu 26 May 2016
ERR580187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410176
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.10923189888146535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47150.044.8856541
GTATCAA75700.035.785191
CAACGCA85000.030.7569565
ATCAACG85550.030.3415973
TATCAAC90850.029.5066742
TCAACGC92850.028.4114844
AACGCAG95850.027.9444926
GTACTAG16400.026.669561
GTACATG133200.023.9740981
ACGCAGA109000.023.8837837
TACATGG134250.023.4008982
CGCAGAG111800.023.1168148
ACATGGG135350.022.6173693
GTATAAG20000.020.6932851
GCATCGT17950.020.4191328
GCAGAGT126500.019.6161699
CATGGGG105100.019.5520214
TAGGCAT24250.019.1960095
CTAGTAC23050.018.9727973
ATGGGGG66050.018.7939725
Produced by FastQC (version 0.11.5)