FastQCFastQC Report
Thu 26 May 2016
ERR580187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3410176
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.044.382261
GTATCAA72750.039.616161
CAACGCA81950.034.4079445
ATCAACG83750.033.614293
TATCAAC88600.032.2506942
TCAACGC91400.030.953744
AACGCAG94050.030.6303396
GTACTAG17100.028.6880761
ACGCAGA104900.026.745397
CGCAGAG107650.025.9311948
GTACATG131400.023.2618261
TACATGG131050.023.2025892
ACATGGG133500.021.9325283
GCAGAGT126150.021.495069
GTGGTAT21500.020.8424761
GCATCGT20300.020.6036458
TGGTATC21050.020.3169482
TAGGCAT25350.019.8363745
CTAGGCA26900.019.5671944
CATGGGG103250.019.4812364
Produced by FastQC (version 0.11.5)