FastQCFastQC Report
Thu 26 May 2016
ERR580186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8604226
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182020.21154720947590172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138420.16087443542277946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123650.047.731251
GTATCAA228900.042.1790121
CAACGCA251250.036.709795
ATCAACG255900.035.96823
TATCAAC281750.033.8435862
AACGCAG292000.032.744676
TCAACGC308850.030.1834664
ACGCAGA304150.030.1400287
CGCAGAG307650.029.5987648
GTACTAG67000.028.0762041
GGGCACC70800.027.55232894
CTGTGCG81900.027.4275139
GTACATG342350.027.308591
TACATGG344400.026.8133262
ACATGGG341700.026.2075443
GCAGAGT338600.026.2033889
TAGTACT88750.026.060984
GCATCGT63250.026.0006548
TAGGCAT83750.024.9222415
TAACGCC32100.024.3106784
Produced by FastQC (version 0.11.5)