FastQCFastQC Report
Thu 26 May 2016
ERR580186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8604226
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159600.1854902463045485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128010.14877572950780232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124100.046.7133141
GTATCAA226600.040.8125571
CAACGCA254600.034.371945
ATCAACG259900.033.726633
TATCAAC286550.031.8804382
GTACTAG67150.031.017521
AACGCAG295850.030.7700486
TCAACGC310700.028.5143184
ACGCAGA307600.028.2501497
CGCAGAG312900.027.6364618
TAGGCAT84950.026.7771745
GTACATG343850.026.0754031
GTGGTAT42250.026.0398521
CTGTGCG82400.025.778719
TACATGG346150.025.576312
GGGCACC73500.025.51191594
ACATGGG339400.025.1342093
GCAGAGT341800.024.8173339
CTAGGCA94300.024.720824
GCATCGT77400.024.2271378
Produced by FastQC (version 0.11.5)