FastQCFastQC Report
Thu 26 May 2016
ERR580185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9059276
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225120.24849667898406008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178660.1972122275554912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112810.12452429973432756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135000.045.606821
GTATCAA235950.039.19111
CAACGCA257950.034.1486365
ATCAACG267750.032.9029353
GTACTAG68300.031.8848361
TATCAAC293300.031.1599432
AACGCAG300900.030.149046
TCAACGC313350.028.4293544
GTACATG363350.028.3752231
ACGCAGA319900.027.5203977
TAGTACT103100.027.5090354
GGGCACC89650.027.46989494
TACATGG366550.027.422512
GTGGTAT45350.027.277361
TAGGCAT85650.027.2348775
CTGTGCG102400.026.8979559
ACATGGG360850.026.8904973
CGCAGAG327600.026.8867118
GCATCGT75850.026.335738
CTAACGC32950.026.2656843
Produced by FastQC (version 0.11.5)