##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR580185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9059276 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.143867236189735 33.0 31.0 34.0 30.0 34.0 2 32.23050760347736 34.0 31.0 34.0 30.0 34.0 3 32.28412700970806 34.0 31.0 34.0 30.0 34.0 4 35.74282779330269 37.0 35.0 37.0 35.0 37.0 5 35.794889459157666 37.0 35.0 37.0 35.0 37.0 6 35.89276604443887 37.0 37.0 37.0 35.0 37.0 7 35.85914426274241 37.0 37.0 37.0 35.0 37.0 8 35.85765164898387 37.0 37.0 37.0 35.0 37.0 9 37.57636504285773 39.0 38.0 39.0 35.0 39.0 10-11 37.5511883620722 39.0 38.0 39.0 35.0 39.0 12-13 37.46817383640812 39.0 38.0 39.0 35.0 39.0 14-15 38.8587538341916 41.0 39.0 41.0 35.0 41.0 16-17 38.84263323029346 41.0 39.0 41.0 35.0 41.0 18-19 38.85496280276702 41.0 39.0 41.0 35.0 41.0 20-21 38.840872935099895 41.0 39.0 41.0 35.0 41.0 22-23 38.76146024251828 40.5 39.0 41.0 35.0 41.0 24-25 38.67112642334774 40.0 38.5 41.0 34.5 41.0 26-27 38.55925567341143 40.0 38.0 41.0 34.0 41.0 28-29 38.47584586229628 40.0 38.0 41.0 34.0 41.0 30-31 38.3444303937754 40.0 38.0 41.0 34.0 41.0 32-33 38.26495351284142 40.0 38.0 41.0 34.0 41.0 34-35 38.14838641630965 40.0 38.0 41.0 33.5 41.0 36-37 38.00373644648866 40.0 38.0 41.0 33.0 41.0 38-39 37.860898652386794 40.0 38.0 41.0 33.0 41.0 40-41 37.704598910553116 40.0 37.0 41.0 33.0 41.0 42-43 37.55224749748214 40.0 37.0 41.0 32.0 41.0 44-45 37.413893670973266 40.0 37.0 41.0 32.0 41.0 46-47 37.172018878771325 40.0 36.0 41.0 31.0 41.0 48-49 36.91783614937883 40.0 36.0 41.0 30.5 41.0 50-51 35.89475693201091 38.0 34.5 40.0 29.0 40.5 52-53 36.073187526243814 38.5 35.0 40.0 29.5 40.5 54-55 36.63958190477915 39.0 35.0 41.0 31.0 41.0 56-57 36.57414201752988 39.0 35.0 41.0 30.5 41.0 58-59 36.345995253925366 39.0 35.0 41.0 30.0 41.0 60-61 36.08279679303291 38.0 35.0 40.5 30.0 41.0 62-63 35.772397705953544 37.0 35.0 40.0 29.0 41.0 64-65 35.51917879530329 37.0 35.0 40.0 29.0 41.0 66-67 35.26378769120181 36.0 35.0 40.0 29.0 41.0 68-69 35.01299425031316 36.0 34.5 39.0 29.0 41.0 70-71 34.70236561950425 35.5 34.0 39.0 29.0 41.0 72-73 34.37659990710074 35.0 34.0 38.5 28.0 41.0 74-75 34.054810947364885 35.0 34.0 37.0 28.0 40.0 76-77 33.76763672946933 35.0 34.0 37.0 27.5 39.0 78-79 33.47054924698177 35.0 34.0 36.5 27.0 39.0 80-81 33.19242956059623 35.0 34.0 36.0 26.5 38.5 82-83 32.93163084997079 35.0 33.0 36.0 26.0 37.0 84-85 32.70564882889096 35.0 33.0 35.0 26.0 37.0 86-87 32.487007295064195 35.0 33.0 35.0 25.0 36.5 88-89 32.33523230774733 35.0 33.0 35.0 25.0 36.0 90-91 32.143557498413784 35.0 33.0 35.0 25.0 36.0 92-93 31.957348743983516 35.0 33.0 35.0 24.0 36.0 94-95 31.825128409819946 35.0 32.5 35.0 24.0 35.5 96-97 31.688882533217885 35.0 32.0 35.0 23.5 35.0 98-99 31.561089594797643 35.0 32.0 35.0 23.0 35.0 100 30.414744842744607 34.0 30.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.048858796175437647 1101 2 0.049623309992625764 1101 3 0.06495870442688556 1101 4 0.015646556495106267 1101 5 0.05788613330003045 1101 6 0.04409884070115311 1101 7 0.06733807473904108 1101 8 0.040747937307386906 1101 9 0.071745964506988 1101 10-11 0.06899923026743693 1101 12-13 0.12456715079672165 1101 14-15 0.07294873924853817 1101 16-17 0.07697284693406203 1101 18-19 0.07695883388384317 1101 20-21 0.09730298532046078 1101 22-23 0.04012543386798484 1101 24-25 0.015792823916463306 1101 26-27 0.10444288948932012 1101 28-29 0.07456390574833449 1101 30-31 0.017711493195612604 1101 32-33 0.09608962745313221 1101 34-35 0.08379397907275887 1101 36-37 0.031503890145671676 1101 38-39 0.04285994708723706 1101 40-41 0.01957339829200322 1101 42-43 0.04305650570835695 1101 44-45 0.025847338514360274 1101 46-47 0.030325908118278733 1101 48-49 0.04428512895993464 1101 50-51 -0.0850614398126055 1101 52-53 0.08347455428516071 1101 54-55 0.14009597639822857 1101 56-57 0.15690285796476644 1101 58-59 0.16404524143299426 1101 60-61 0.10363225078025806 1101 62-63 0.10264801048587202 1101 64-65 0.07364593869564118 1101 66-67 0.09743608736584264 1101 68-69 0.06800023519851095 1101 70-71 0.08244024256632088 1101 72-73 0.10157298143298021 1101 74-75 0.0542256735997384 1101 76-77 0.15708533709795347 1101 78-79 0.07570007554257785 1101 80-81 0.09162484527789871 1101 82-83 0.07914706039871788 1101 84-85 0.05736340451242228 1101 86-87 0.06795735686362292 1101 88-89 7.695145757011801E-4 1101 90-91 -0.004642929927648254 1101 92-93 -0.027604260041073303 1101 94-95 0.07026919135904564 1101 96-97 -0.019820833794014447 1101 98-99 -0.024490702225790528 1101 100 -9.889881227067576E-4 1102 1 0.040505236420123936 1102 2 0.031225525516418884 1102 3 0.06214152295852671 1102 4 0.03698291044060653 1102 5 0.028441574144508763 1102 6 0.02209005174820078 1102 7 0.007649092260756163 1102 8 0.056809524613527174 1102 9 0.03453102616511927 1102 10-11 0.06756574431053508 1102 12-13 0.12416155205697521 1102 14-15 0.09287285566275472 1102 16-17 0.10502943756333138 1102 18-19 0.06862624628163161 1102 20-21 0.0623521370238862 1102 22-23 0.08730213265659614 1102 24-25 0.12432842515322307 1102 26-27 0.05726377178141462 1102 28-29 0.04841702120833702 1102 30-31 -0.04629418583751743 1102 32-33 -0.004570801365026966 1102 34-35 0.059201194927588574 1102 36-37 -0.0019894549039136677 1102 38-39 0.047691455974224084 1102 40-41 0.014330605590977541 1102 42-43 -0.015764907419878682 1102 44-45 0.008849905712189354 1102 46-47 -0.03537690065713406 1102 48-49 0.030124984125357912 1102 50-51 -0.0123350839751879 1102 52-53 0.06643793376247231 1102 54-55 0.1618439579635691 1102 56-57 0.13728719673683543 1102 58-59 0.11907245439788738 1102 60-61 0.08511558931325425 1102 62-63 0.1265922971016309 1102 64-65 0.12782075519667302 1102 66-67 0.14478981543864933 1102 68-69 0.09883166392963005 1102 70-71 0.13168040230438294 1102 72-73 0.1574106023519306 1102 74-75 0.1206369678752921 1102 76-77 0.14480918045178015 1102 78-79 0.045062866385080724 1102 80-81 0.08683722074407996 1102 82-83 0.11095980294141583 1102 84-85 0.10209825338423428 1102 86-87 0.09470119262228849 1102 88-89 0.08195002405854979 1102 90-91 0.011786241021042088 1102 92-93 0.052399439063634645 1102 94-95 0.05267073078384499 1102 96-97 -0.009640173429538379 1102 98-99 -0.0025598957929702237 1102 100 0.08243219577547123 1103 1 0.06575450147026629 1103 2 0.03389371908281191 1103 3 0.04453313937200676 1103 4 0.0330988305804496 1103 5 0.04596803051488507 1103 6 0.07492353801924878 1103 7 0.03025541436422685 1103 8 0.07068887129444335 1103 9 0.09229466847755674 1103 10-11 0.10865494645111795 1103 12-13 0.13292218073418383 1103 14-15 0.1373928042198358 1103 16-17 0.08805446742014311 1103 18-19 0.08055681017727778 1103 20-21 0.08388016369234919 1103 22-23 0.059420835270721284 1103 24-25 0.05199687165296751 1103 26-27 0.03175790600387529 1103 28-29 0.07653271976758447 1103 30-31 0.10225389193434609 1103 32-33 0.08752532995323747 1103 34-35 0.0868754897100601 1103 36-37 0.061550355151140934 1103 38-39 0.06341636178569132 1103 40-41 0.0473971342127939 1103 42-43 0.09397702575463995 1103 44-45 0.1306739614198662 1103 46-47 0.16574481022234266 1103 48-49 0.1388597971254839 1103 50-51 0.06069952659404976 1103 52-53 0.10877374032627074 1103 54-55 0.23673864463394523 1103 56-57 0.20969163409451141 1103 58-59 0.2507165115083225 1103 60-61 0.23706688678687016 1103 62-63 0.22442157613614455 1103 64-65 0.18162977700999505 1103 66-67 0.19483302280043802 1103 68-69 0.20139001546958468 1103 70-71 0.16828015653737793 1103 72-73 0.1344958419148341 1103 74-75 0.10261371868144664 1103 76-77 0.12681781489448696 1103 78-79 0.13373990635781752 1103 80-81 0.1657200835341186 1103 82-83 0.1314327283763177 1103 84-85 0.16061247006284418 1103 86-87 0.11199925231658625 1103 88-89 0.10802851384644185 1103 90-91 0.05787061131092486 1103 92-93 0.06782283246062093 1103 94-95 0.029791850879369974 1103 96-97 0.059281624986233794 1103 98-99 0.07001009238951639 1103 100 0.2011535922633776 1104 1 0.025692713726215288 1104 2 0.013338972174189223 1104 3 0.014882089659550957 1104 4 0.015926712043977886 1104 5 -0.012773501303747992 1104 6 0.023737747620636185 1104 7 0.04636324919976431 1104 8 -0.005398356574659147 1104 9 0.003185019220666163 1104 10-11 0.06758736599635284 1104 12-13 0.05146477663173954 1104 14-15 0.03995550556182792 1104 16-17 0.10282858944093931 1104 18-19 0.08401658546558366 1104 20-21 0.06500947825765735 1104 22-23 0.06837326620674133 1104 24-25 0.058511982965150366 1104 26-27 0.029112000816887473 1104 28-29 0.09030348696410329 1104 30-31 0.07756193595028549 1104 32-33 0.09116313799298581 1104 34-35 0.09827057924119487 1104 36-37 0.07185396635818364 1104 38-39 0.042113397057370605 1104 40-41 0.0614573150971367 1104 42-43 0.10680972472553663 1104 44-45 0.03611412449691187 1104 46-47 0.07254212639217172 1104 48-49 0.12606862145523934 1104 50-51 0.057340826077236784 1104 52-53 0.12789890453620956 1104 54-55 0.2220665908233599 1104 56-57 0.2013173235470873 1104 58-59 0.19726457158701294 1104 60-61 0.21415528269611883 1104 62-63 0.210669117466729 1104 64-65 0.22529485841663188 1104 66-67 0.2353798280502204 1104 68-69 0.18430925672716114 1104 70-71 0.20496274723443264 1104 72-73 0.1651063340221981 1104 74-75 0.15395310202001866 1104 76-77 0.16065895143908904 1104 78-79 0.1393889828937489 1104 80-81 0.18669997955241513 1104 82-83 0.1277542198225703 1104 84-85 0.16370421592059614 1104 86-87 0.09990845703951123 1104 88-89 0.1416522640578748 1104 90-91 0.09053473019123714 1104 92-93 0.07172015351109451 1104 94-95 0.08921783150333695 1104 96-97 0.19087563849280897 1104 98-99 0.10905642120315662 1104 100 0.12822163547773968 1105 1 0.0823563952099704 1105 2 0.028080502136418772 1105 3 0.06446562840964987 1105 4 0.06774928153897264 1105 5 0.02700202710664712 1105 6 0.04862818164555449 1105 7 0.08186125169223146 1105 8 0.049303588847699586 1105 9 0.08106259341452215 1105 10-11 0.11484924071228164 1105 12-13 0.16190857637622003 1105 14-15 0.1223979737941292 1105 16-17 0.14132355908711958 1105 18-19 0.10412230439266779 1105 20-21 0.1384835367884918 1105 22-23 0.1153022987710699 1105 24-25 0.13679525825538974 1105 26-27 0.1448387679537646 1105 28-29 0.1587126046066345 1105 30-31 0.09770037560216593 1105 32-33 0.08139744326702925 1105 34-35 0.08144232842342802 1105 36-37 0.10211312359098912 1105 38-39 0.14847935703294723 1105 40-41 0.07716713923932872 1105 42-43 0.042988268046244116 1105 44-45 0.017834210049819887 1105 46-47 0.06405233655039666 1105 48-49 0.01939869970455277 1105 50-51 -0.012317134555672737 1105 52-53 0.009145009753041222 1105 54-55 0.09101488379929634 1105 56-57 0.06396072167486722 1105 58-59 0.07355336137816693 1105 60-61 0.035959208436558754 1105 62-63 0.01717681620463196 1105 64-65 0.05989754052546914 1105 66-67 0.0866254551975274 1105 68-69 0.12638725700108466 1105 70-71 0.1020064183834748 1105 72-73 0.06146794256010679 1105 74-75 0.013237296026858303 1105 76-77 0.02650253216167897 1105 78-79 0.008100861155710959 1105 80-81 0.06757377436496625 1105 82-83 0.03489654748616289 1105 84-85 0.0171240903668064 1105 86-87 0.04941675367538778 1105 88-89 0.02441524704328657 1105 90-91 0.05642803445181954 1105 92-93 0.012502257554917406 1105 94-95 -0.008057401882169302 1105 96-97 -0.05982189318492104 1105 98-99 -0.008070912592071267 1105 100 -0.07153634361755579 1106 1 0.011956756185341533 1106 2 0.03037239136533998 1106 3 0.008964114880612328 1106 4 0.005263029844606137 1106 5 0.027965694377428463 1106 6 -0.009892709930923616 1106 7 0.014448438672992836 1106 8 0.031621347790391496 1106 9 0.03269082260175793 1106 10-11 0.05255150198004088 1106 12-13 0.0508805716148828 1106 14-15 0.02755058861793458 1106 16-17 0.0535774159756599 1106 18-19 0.05495061294487158 1106 20-21 0.01120783550688742 1106 22-23 0.054581149850271515 1106 24-25 0.0399851681369654 1106 26-27 0.05521630259174515 1106 28-29 0.03934571082253768 1106 30-31 0.012294527028913649 1106 32-33 0.05550426666825814 1106 34-35 0.05740458299194984 1106 36-37 0.0721114847154567 1106 38-39 0.049760040327086585 1106 40-41 0.1430836504382924 1106 42-43 0.1535608965137243 1106 44-45 0.08471121786612201 1106 46-47 0.12661545897608306 1106 48-49 0.179217142451499 1106 50-51 0.09554278851158671 1106 52-53 0.14638403150640045 1106 54-55 0.13529168522718749 1106 56-57 0.20083314543087027 1106 58-59 0.15668412518576247 1106 60-61 0.18338043143791793 1106 62-63 0.20146731344623703 1106 64-65 0.16486399571659405 1106 66-67 0.1554487510170972 1106 68-69 0.1851274168781174 1106 70-71 0.18199164736594753 1106 72-73 0.1577830232166022 1106 74-75 0.18872073964960379 1106 76-77 0.13797724676411605 1106 78-79 0.15440654258620867 1106 80-81 0.12760280486792652 1106 82-83 0.14025412039463703 1106 84-85 0.1479121006183064 1106 86-87 0.10579269819543669 1106 88-89 0.10718480296437605 1106 90-91 0.11155231067970561 1106 92-93 0.13223116950361202 1106 94-95 0.15354816206505006 1106 96-97 0.09567628763505098 1106 98-99 0.054783346278959755 1106 100 0.0863651572852362 1107 1 0.06829463408252678 1107 2 0.02206909594637807 1107 3 0.007089386407315601 1107 4 0.010941747241353994 1107 5 0.011557705930954398 1107 6 0.028524998038491844 1107 7 0.03957268720213136 1107 8 0.07379715972061973 1107 9 0.05648336120474795 1107 10-11 0.05065609737996368 1107 12-13 0.08266978530143376 1107 14-15 0.08150415694085211 1107 16-17 0.09690335053310406 1107 18-19 0.09288872542719417 1107 20-21 0.14645697063112806 1107 22-23 0.10450524440676645 1107 24-25 0.10590149711680397 1107 26-27 0.12415141581532652 1107 28-29 0.13994646910990127 1107 30-31 0.09822576513490588 1107 32-33 0.09825649972481898 1107 34-35 0.14831357246978882 1107 36-37 0.1573543786479732 1107 38-39 0.15524423477054228 1107 40-41 0.12683953685836258 1107 42-43 0.11912907856963528 1107 44-45 0.14099411051552835 1107 46-47 0.16698299892146196 1107 48-49 0.05511515928667876 1107 50-51 0.04508118439282072 1107 52-53 0.11172313741907658 1107 54-55 0.15896072494138735 1107 56-57 0.2040571916617111 1107 58-59 0.16148956387878144 1107 60-61 0.18283507680224176 1107 62-63 0.15536376963481047 1107 64-65 0.1566815492949658 1107 66-67 0.162026583559296 1107 68-69 0.19175552762405346 1107 70-71 0.19107498714966908 1107 72-73 0.17392867240862842 1107 74-75 0.11206189016274237 1107 76-77 0.13822187358618265 1107 78-79 0.09498746852801787 1107 80-81 0.1300387524586526 1107 82-83 0.15441848758312204 1107 84-85 0.11262074156568502 1107 86-87 0.10545194307399441 1107 88-89 0.1014884777362326 1107 90-91 0.009874057711307671 1107 92-93 0.0043436071875788684 1107 94-95 0.07831858035041961 1107 96-97 0.09821307056919437 1107 98-99 0.03505493923331926 1107 100 0.03314306360599417 1108 1 0.021462471354993795 1108 2 0.036485084099247445 1108 3 0.06322185436066263 1108 4 0.05437743252034721 1108 5 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98-99 -0.18022836697647193 2316 100 -0.33551489118868005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 713.0 1 323.0 2 242.0 3 259.0 4 266.0 5 217.0 6 333.0 7 1010.0 8 1867.0 9 2770.0 10 3846.0 11 6564.0 12 9796.0 13 12892.0 14 15190.0 15 16907.0 16 19800.0 17 23782.0 18 30069.0 19 36307.0 20 45000.0 21 57236.0 22 65907.0 23 67366.0 24 63831.0 25 61598.0 26 64461.0 27 72242.0 28 83504.0 29 100217.0 30 122407.0 31 153477.0 32 197966.0 33 266071.0 34 417557.0 35 645319.0 36 1196704.0 37 2239486.0 38 2237481.0 39 717717.0 40 576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49159774381478 19.515158129932257 11.915230995625297 24.078013130627664 2 15.944748842958468 19.870331352474842 40.380586474511276 23.804333330055414 3 19.06204989914426 25.976958151083057 29.002177334833878 25.958814614938802 4 12.645710841706578 15.88970056992518 38.6659215595356 32.79866702883264 5 13.18955332268711 38.14237018892259 34.79245609095582 13.875620397434474 6 31.07494106378827 37.20891220876036 17.605039088896472 14.111107638554902 7 27.470345167583154 31.958029554996397 22.83599901400885 17.7356262634116 8 26.306528012821108 35.11885277946008 19.909778700723173 18.66484050699564 9 26.799125383748923 14.812984517525454 20.95629127371513 37.43159882501049 10-11 25.817759466851918 25.53628226255772 27.643468759867645 21.002489510722718 12-13 25.967374747480772 23.4241331415435 27.946140494664935 22.662351616310794 14-15 23.24995480501886 24.67118649608198 25.55105022976278 26.52780846913638 16-17 22.32633233814452 27.41263350546047 26.589187185419277 23.671846970975725 18-19 21.876679069744846 27.32246332243638 28.63639164281785 22.164465965000925 20-21 23.191092540439307 26.536540693920657 28.10357086407929 22.168795901560742 22-23 23.449494847720032 26.25162074118078 27.801398509511614 22.497485901587574 24-25 22.93591399825241 26.31309568150475 28.392301645257255 22.35868867498558 26-27 22.84699047318809 26.566469188654395 27.665253375212178 22.921286962945338 28-29 22.274008524220868 26.824271432851628 28.1504883117276 22.751231731199905 30-31 22.957792329492463 26.567912171822535 28.075865639727976 22.398429858957034 32-33 23.121565192024626 26.74714016506401 27.44859650763537 22.68269813527599 34-35 23.070905262093227 26.65615370978685 28.03315120333937 22.239789824780555 36-37 22.891173995150638 26.31668564848586 27.762075539910736 23.030064816452764 38-39 23.005166957860734 26.55254288475644 28.0771391445713 22.365151012811527 40-41 22.801593531524855 25.872354597812624 28.456457236411115 22.86959463425141 42-43 22.71827655603598 26.0616164652138 27.932414040138344 23.287692938611883 44-45 22.759810013510908 26.733327992063856 27.83921372684906 22.667648267576176 46-47 22.846485664237687 26.58336082556764 27.340241591172298 23.22991191902237 48-49 23.325135634173616 26.49597887230859 27.32925763652062 22.849627856997174 50-51 23.00605548487857 26.482742947529754 27.3023008943177 23.20890067327398 52-53 22.747456246152815 26.848869360523835 27.12616497841379 23.27750941490956 54-55 22.630457687032283 26.71816758235873 27.196628557181562 23.454746173427417 56-57 23.340782937168257 26.480073229900146 26.85518323485796 23.32396059807363 58-59 22.675035497326935 26.792764065884807 27.44638413076266 23.085816306025603 60-61 22.804098647088733 27.19262769076535 26.9661211851209 23.037152477025018 62-63 22.96495138149236 26.67653981242238 26.789978304363952 23.568530501721312 64-65 22.471339887680923 26.750354568192495 27.099294606516942 23.679010937609636 66-67 22.868849164858357 26.52441697086074 26.945565030330343 23.661168833950555 68-69 22.511326832252458 26.58080395939456 27.22307372075535 23.68479548759763 70-71 22.590017472473743 26.258767639841214 27.30828660157487 23.842928286110173 72-73 23.01327569419467 26.369530472409842 27.36660725105989 23.250586582335597 74-75 23.11509608179557 26.638457806186256 27.01059767419622 23.23584843782195 76-77 22.918083782628447 26.523348246624195 26.845104995077396 23.713462975669966 78-79 22.80710713734216 26.518993012920056 26.86486056453477 23.809039285203013 80-81 22.486089368680798 26.93024795250027 27.22788591253383 23.355776766285107 82-83 23.16428148243346 26.24903915818222 27.945365225884224 22.641314133500096 84-85 23.316163019589993 26.461204852697946 27.028739542827285 23.19389258488478 86-87 22.333363353402603 27.006731655423128 27.390953570843063 23.2689514203312 88-89 23.105743504312603 26.121720337366312 27.321303888922788 23.4512322693983 90-91 22.899495759937512 26.23388407966995 27.361110911721383 23.505509248671153 92-93 23.23443328291097 26.30372615290631 27.277542524956637 23.18429803922608 94-95 22.390046963775024 26.321037340725862 27.72393235524665 23.564983340252464 96-97 22.669540481683157 26.17646940274308 27.406040168588163 23.7479499469856 98-99 22.256453565923653 26.619874157804134 27.3004054664902 23.823266809782016 100 22.888336280744472 26.144937530087503 26.653218240545197 24.31350794862283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1432.0 1 1007.0 2 781.0 3 996.0 4 846.0 5 790.0 6 1224.0 7 2168.0 8 3399.0 9 4154.5 10 3922.0 11 3647.0 12 4089.5 13 4763.0 14 5503.0 15 6404.5 16 7221.5 17 8121.0 18 9033.5 19 9686.5 20 10519.5 21 11526.0 22 12676.0 23 14431.0 24 16696.0 25 19964.5 26 25163.5 27 30863.0 28 37973.5 29 51508.0 30 71493.5 31 90392.5 32 114788.0 33 141604.5 34 164674.5 35 189274.5 36 215171.0 37 251285.0 38 266618.5 39 263059.0 40 257485.5 41 245848.5 42 248577.5 43 264013.5 44 283690.0 45 321924.5 46 359042.5 47 383995.0 48 402785.0 49 402398.5 50 392652.5 51 398762.0 52 399094.0 53 384395.0 54 368396.0 55 327880.5 56 291860.5 57 248250.0 58 203763.5 59 189538.5 60 159641.0 61 119407.0 62 92313.5 63 74161.0 64 54190.0 65 31923.5 66 20530.5 67 14518.5 68 10590.5 69 7643.0 70 5549.5 71 4211.5 72 3187.5 73 2407.5 74 1823.5 75 1266.5 76 867.0 77 586.0 78 373.0 79 246.5 80 181.5 81 154.5 82 131.0 83 112.0 84 101.0 85 82.0 86 78.5 87 71.5 88 63.0 89 61.5 90 46.0 91 36.0 92 32.0 93 29.5 94 31.0 95 30.0 96 21.5 97 17.0 98 18.5 99 15.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07732405989176176 2 0.09739188871163655 3 0.10178517576901289 4 0.11058278829345744 5 0.10411428021400385 6 0.045776284992310644 7 0.048878078115734636 8 0.04644962798351656 9 0.04278487596580566 10-11 0.03841918493265908 12-13 0.03896558621240814 14-15 0.04489321221695862 16-17 0.03723255589077979 18-19 0.032254233119732745 20-21 0.027182083866304545 22-23 0.031442910007378075 24-25 0.03716632543262839 26-27 0.03304347941270362 28-29 0.028837845320089598 30-31 0.0220823385886466 32-33 0.028114829485270124 34-35 0.03126629545230767 36-37 0.03578652422114085 38-39 0.035030393157245675 40-41 0.0390814895141731 42-43 0.03198931128712714 44-45 0.044159157972447245 46-47 0.04071517414857434 48-49 0.038446780956888825 50-51 0.03975483250537901 52-53 0.04369002556054148 54-55 0.03022316573642309 56-57 0.031636082176986324 58-59 0.02490265226492713 60-61 0.023633235150358593 62-63 0.02633212632002822 64-65 0.023539408667977442 66-67 0.02218720348071965 68-69 0.036934518829098484 70-71 0.03977690932476282 72-73 0.04255306936227575 74-75 0.034638529613183214 76-77 0.02858396189717589 78-79 0.030626067690177446 80-81 0.030946181571242556 82-83 0.023335198088677286 84-85 0.03896558621240814 86-87 0.04660968492404912 88-89 0.04562726646146999 90-91 0.04268553027857855 92-93 0.04161480453846422 94-95 0.03826464719697247 96-97 0.041035288029639454 98-99 0.03764649625422605 100 0.04897742380296174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9059276.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.99176075032838 #Duplication Level Percentage of deduplicated Percentage of total 1 74.40084159545813 44.634374886178044 2 15.252789853366117 18.30083439316353 3 4.879649362624977 8.782162713242698 4 2.1250016733663197 5.099303679305588 5 1.019514839966447 3.058124518033821 6 0.6040547851404945 2.1742986090143734 7 0.37229550432595954 1.5634263976762066 8 0.2679903208994028 1.286176897184056 9 0.18638104990205343 1.0063194618707103 >10 0.8287146032478974 8.510286197784671 >50 0.04178335637906218 1.73034110319309 >100 0.01913755292397329 2.1124978244602395 >500 0.0013072527803317641 0.5303121772597921 >1k 4.613568169293826E-4 0.4172091830762545 >5k 0.0 0.0 >10k+ 7.689280201513078E-5 0.7943319585570005 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22512 0.24849667898406008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17866 0.1972122275554912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11281 0.12452429973432756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.2076819383800648E-5 10-11 0.0 0.0 0.0 0.0 3.311522907570097E-5 12-13 0.0 0.0 0.0 0.0 3.311522907570097E-5 14-15 0.0 0.0 0.0 0.0 5.519204845950162E-5 16-17 0.0 0.0 0.0 1.6557614537850486E-5 6.623045815140194E-5 18-19 0.0 0.0 0.0 3.311522907570097E-5 7.726886784330227E-5 20-21 0.0 0.0 0.0 4.967284361355146E-5 7.726886784330227E-5 22-23 0.0 0.0 0.0 1.4349932599470422E-4 1.0486489207305307E-4 24-25 0.0 5.519204845950162E-6 0.0 3.863443392165114E-4 1.5453773568660455E-4 26-27 0.0 1.1038409691900324E-5 0.0 8.002847026627735E-4 1.5453773568660455E-4 28-29 0.0 1.1038409691900324E-5 0.0 0.003041081870118539 1.5453773568660455E-4 30-31 0.0 1.1038409691900324E-5 0.0 0.011871809623638799 1.600569405325547E-4 32-33 0.0 1.1038409691900324E-5 0.0 0.03314834430477667 1.6557614537850485E-4 34-35 0.0 1.1038409691900324E-5 0.0 0.06851540895762531 1.876529647623055E-4 36-37 0.0 2.2076819383800648E-5 0.0 0.12549016058236884 1.931721696082557E-4 38-39 0.0 3.311522907570097E-5 0.0 0.20002702202692577 1.9869137445420584E-4 40-41 0.0 3.311522907570097E-5 0.0 0.2741113086741148 1.9869137445420584E-4 42-43 0.0 3.311522907570097E-5 0.0 0.3485377860217528 2.04210579300156E-4 44-45 0.0 3.311522907570097E-5 0.0 0.44172955984562123 2.2076819383800648E-4 46-47 0.0 3.311522907570097E-5 0.0 0.5516776395817944 2.4284501322180714E-4 48-49 0.0 3.311522907570097E-5 0.0 0.6690656074503084 2.7596024229750813E-4 50-51 0.0 3.311522907570097E-5 0.0 0.790813747147123 2.7596024229750813E-4 52-53 0.0 3.311522907570097E-5 0.0 0.9052158251939779 2.8699865198940843E-4 54-55 0.0 4.4153638767601295E-5 0.0 1.0335814915010868 2.9803706168130873E-4 56-57 0.0 4.4153638767601295E-5 0.0 1.1865296961920577 3.035562665272589E-4 58-59 0.0 4.4153638767601295E-5 0.0 1.3608261852271637 3.090754713732091E-4 60-61 0.0 4.4153638767601295E-5 0.0 1.5389640408350513 3.090754713732091E-4 62-63 0.0 4.4153638767601295E-5 0.0 1.7197566339738408 3.311522907570097E-4 64-65 0.0 4.4153638767601295E-5 0.0 1.9076745205687518 3.311522907570097E-4 66-67 0.0 4.4153638767601295E-5 0.0 2.1171780173161743 3.4219070044891006E-4 68-69 0.0 4.4153638767601295E-5 0.0 2.353112986070852 3.5322911014081036E-4 70-71 0.0 4.4153638767601295E-5 0.0 2.611698771513309 3.9186354406246153E-4 72-73 0.0 4.4153638767601295E-5 0.0 2.8741480003479305 4.8569002644361427E-4 74-75 0.0 4.4153638767601295E-5 0.0 3.1331090917199123 4.8569002644361427E-4 76-77 0.0 4.4153638767601295E-5 0.0 3.417905580975787 4.912092312895645E-4 78-79 0.0 4.4153638767601295E-5 0.0 3.7306347659570145 4.967284361355146E-4 80-81 0.0 5.519204845950162E-5 0.0 4.071511895652589 5.298436652112156E-4 82-83 0.0 5.519204845950162E-5 0.0 4.423946240295582 5.629588942869166E-4 84-85 0.0 5.519204845950162E-5 0.0 4.770094210619039 5.629588942869166E-4 86-87 0.0 5.519204845950162E-5 0.0 5.13538278334825 5.629588942869166E-4 88 0.0 5.519204845950162E-5 0.0 5.430698877040506 5.629588942869166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13500 0.0 45.60682 1 GTATCAA 23595 0.0 39.1911 1 CAACGCA 25795 0.0 34.148636 5 ATCAACG 26775 0.0 32.902935 3 GTACTAG 6830 0.0 31.884836 1 TATCAAC 29330 0.0 31.159943 2 AACGCAG 30090 0.0 30.14904 6 TCAACGC 31335 0.0 28.429354 4 GTACATG 36335 0.0 28.375223 1 ACGCAGA 31990 0.0 27.520397 7 TAGTACT 10310 0.0 27.509035 4 GGGCACC 8965 0.0 27.469894 94 TACATGG 36655 0.0 27.42251 2 GTGGTAT 4535 0.0 27.27736 1 TAGGCAT 8565 0.0 27.234877 5 CTGTGCG 10240 0.0 26.897955 9 ACATGGG 36085 0.0 26.890497 3 CGCAGAG 32760 0.0 26.886711 8 GCATCGT 7585 0.0 26.33573 8 CTAACGC 3295 0.0 26.265684 3 >>END_MODULE