FastQCFastQC Report
Thu 26 May 2016
ERR580185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9059276
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194480.21467499168807752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166300.1835687531763024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106510.11757010162843035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137850.044.8446161
GTATCAA239600.038.632271
CAACGCA272100.032.4042635
GTACTAG67900.032.1247331
ATCAACG278900.031.6819523
TATCAAC308300.029.7904872
GGGCACC90900.029.42299894
AACGCAG311750.029.0655946
CTGTGCG102000.028.9359749
GTACATG367950.028.3632181
TAGTACT105500.028.1108974
TACATGG369600.027.6855412
TAGGCAT86950.027.3513495
TCAACGC327050.027.2328364
ACATGGG364250.026.8390033
CTAGGCA94250.026.529444
ACGCAGA338350.026.0443637
CTAGTAC118000.025.7307913
CGCAGAG348000.025.2411258
CATGGGG289100.025.2314054
Produced by FastQC (version 0.11.5)