FastQCFastQC Report
Thu 26 May 2016
ERR580184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809194
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99230.1708154349811695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79230.13638725096803447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83350.048.855531
GTATCAA143650.040.393451
CAACGCA165950.033.5154725
ATCAACG170700.032.558113
TATCAAC179650.031.8805122
AACGCAG186200.030.5627776
TCAACGC190300.029.3769764
ACGCAGA206600.026.7941257
CGCAGAG215150.025.7074938
GTACTAG28250.025.1339041
GTACATG237200.023.3920751
GCAGAGT237450.022.723189
TACATGG242500.022.6289462
ACATGGG240050.022.2315313
CATGGGG181500.020.3874134
GAGTACT99950.020.13107712-13
CAGAGTA231950.019.53510510-11
AGAGTAC215850.019.1630110-11
CTGTGCG41750.019.1377939
TAGTACT41050.019.0133634
Produced by FastQC (version 0.11.5)