FastQCFastQC Report
Thu 26 May 2016
ERR580184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809194
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86890.14957324544506517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75630.13019017784567016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.049.6430241
GTATCAA149700.040.3003771
CAACGCA164850.034.9542545
ATCAACG169200.034.0845573
TATCAAC184300.032.4376262
TCAACGC183450.031.6157474
AACGCAG186650.031.5984426
GTACTAG27100.028.0231511
ACGCAGA206950.027.681417
CGCAGAG215250.026.4395878
GTACATG237750.023.9070991
GCAGAGT238500.023.3892359
TACATGG241900.022.9262732
ACATGGG241000.022.1356533
GTGGTAT36300.021.180751
CATGGGG182650.020.7663364
GAGTACT97750.020.72018412-13
CAGAGTA235350.019.58773410-11
GTGATCG19550.019.230788
GTATAAG35800.019.1049231
Produced by FastQC (version 0.11.5)