##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580184_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5809194
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.539857508632004	34.0	31.0	34.0	31.0	34.0
2	32.73447882787182	34.0	31.0	34.0	31.0	34.0
3	32.81725554354012	34.0	31.0	34.0	31.0	34.0
4	36.28442672081531	37.0	37.0	37.0	35.0	37.0
5	36.21704990399701	37.0	37.0	37.0	35.0	37.0
6	36.2452854905517	37.0	37.0	37.0	35.0	37.0
7	36.214608773609555	37.0	37.0	37.0	35.0	37.0
8	36.20398544100954	37.0	37.0	37.0	35.0	37.0
9	37.99205277014333	39.0	38.0	39.0	35.0	39.0
10-11	37.941793043923134	39.0	38.0	39.0	35.0	39.0
12-13	37.91003519593252	39.0	38.0	39.0	35.0	39.0
14-15	39.31796295665113	41.0	39.0	41.0	36.0	41.0
16-17	39.30526033387764	41.0	39.0	41.0	36.0	41.0
18-19	39.300909816404825	41.0	39.0	41.0	36.0	41.0
20-21	39.28425845306595	41.0	39.0	41.0	36.0	41.0
22-23	39.21288874153626	41.0	39.0	41.0	36.0	41.0
24-25	39.13454603168701	40.5	39.0	41.0	35.5	41.0
26-27	39.00441111107668	40.0	38.5	41.0	35.0	41.0
28-29	38.902454281953744	40.0	38.0	41.0	35.0	41.0
30-31	38.80140480762047	40.0	38.0	41.0	35.0	41.0
32-33	38.67098292809639	40.0	38.0	41.0	35.0	41.0
34-35	38.649013529243476	40.0	38.0	41.0	34.5	41.0
36-37	38.70596557801306	40.0	38.0	41.0	35.0	41.0
38-39	38.62042927125519	40.0	38.0	41.0	35.0	41.0
40-41	38.50182779228926	40.0	38.0	41.0	34.0	41.0
42-43	38.48318854560547	40.0	38.0	41.0	34.0	41.0
44-45	38.40435273809069	40.0	38.0	41.0	34.0	41.0
46-47	38.22638966782655	40.0	38.0	41.0	34.0	41.0
48-49	38.03076803770024	40.0	37.0	41.0	33.0	41.0
50-51	37.86074479523321	40.0	37.0	41.0	33.0	41.0
52-53	37.681436357608305	40.0	36.5	41.0	33.0	41.0
54-55	37.48462325410375	40.0	36.0	41.0	33.0	41.0
56-57	37.271225319725936	39.0	35.5	41.0	32.0	41.0
58-59	37.03802524067883	39.0	35.0	41.0	32.0	41.0
60-61	36.776007738767206	38.5	35.0	40.5	32.0	41.0
62-63	36.48943553959465	38.0	35.0	40.0	31.0	41.0
64-65	36.19482169127077	37.0	35.0	40.0	31.0	41.0
66-67	35.863294632611684	37.0	35.0	40.0	31.0	41.0
68-69	35.50788457056177	36.0	35.0	39.0	30.5	41.0
70-71	35.18004916000395	36.0	34.0	39.0	30.0	40.5
72-73	34.826514745419075	35.0	34.0	38.5	30.0	40.0
74-75	34.46985571492362	35.0	34.0	37.0	29.5	39.5
76-77	33.244653044811386	34.5	32.5	36.0	28.0	39.0
78-79	33.86821381761394	35.0	34.0	36.0	29.0	39.0
80-81	33.800096536627976	35.0	34.0	36.0	29.0	37.5
82-83	33.594044371732124	35.0	34.0	36.0	29.0	37.0
84-85	33.37827717580098	35.0	34.0	35.0	29.0	37.0
86-87	33.21295553221324	35.0	34.0	35.0	29.0	36.0
88-89	33.057254414295684	35.0	33.5	35.0	29.0	36.0
90-91	32.90282231924084	35.0	33.0	35.0	29.0	36.0
92-93	32.7942987443697	35.0	33.0	35.0	29.0	35.0
94-95	32.70092735756458	35.0	33.0	35.0	29.0	35.0
96-97	32.60731084208928	35.0	33.0	35.0	28.0	35.0
98-99	32.53565666768918	35.0	33.0	35.0	27.0	35.0
100	31.25948625575252	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.18971387159126607
1101	2	0.10339320158467302
1101	3	0.10461286130313141
1101	4	0.08952999858895083
1101	5	0.041411159660526664
1101	6	0.0993725986168883
1101	7	0.06387381745850007
1101	8	0.07843764026753774
1101	9	0.03709030461238427
1101	10-11	0.09557023298149403
1101	12-13	0.027262266522171785
1101	14-15	0.05551724647701661
1101	16-17	0.0035822279337978102
1101	18-19	0.0442253996093811
1101	20-21	0.049989763829174194
1101	22-23	0.04737587479342409
1101	24-25	-0.013895603583499394
1101	26-27	0.0301844768943198
1101	28-29	-0.010162201969663442
1101	30-31	-0.02560615251716314
1101	32-33	0.045115038102188976
1101	34-35	-0.003999985767940473
1101	36-37	0.06713969884430071
1101	38-39	0.11214117477009466
1101	40-41	0.14264639327144124
1101	42-43	0.08711963401847811
1101	44-45	-0.019961713977103557
1101	46-47	0.07330469414504392
1101	48-49	-0.01174500392184541
1101	50-51	-0.07383664075289431
1101	52-53	-0.04272906925061193
1101	54-55	-0.021422977084959882
1101	56-57	-0.016321305653896445
1101	58-59	-0.0930851599321798
1101	60-61	-0.10745737614776374
1101	62-63	-0.1250201027973361
1101	64-65	-0.1030075021475696
1101	66-67	-0.10348124877793197
1101	68-69	-0.09142171374700325
1101	70-71	-0.12337562440065852
1101	72-73	-0.1306224655600019
1101	74-75	-0.10394798515466164
1101	76-77	-0.09461786473442402
1101	78-79	0.04468313331033613
1101	80-81	-0.011827696469822513
1101	82-83	0.0388494451320156
1101	84-85	-0.042471507535431385
1101	86-87	-0.01707768188511949
1101	88-89	0.025142195915179855
1101	90-91	-0.019742418082714153
1101	92-93	-0.12790939266302814
1101	94-95	-0.0922812004542024
1101	96-97	-0.0821327267033709
1101	98-99	-0.0788298333502766
1101	100	0.032507838283908796
1102	1	0.05321396025209424
1102	2	-0.009210914077691257
1102	3	-0.03007309085699461
1102	4	-0.02513970759952855
1102	5	-0.059886937014077546
1102	6	-0.036124557547545066
1102	7	-0.007578843375767974
1102	8	-0.05255909715995699
1102	9	-0.019179949643515215
1102	10-11	-0.04936185209495392
1102	12-13	-0.08378356516625729
1102	14-15	-0.043742441694696765
1102	16-17	-0.0722422083037415
1102	18-19	-0.09149584101951547
1102	20-21	-0.1741627164970012
1102	22-23	-0.1402278212806678
1102	24-25	-0.1368242969272231
1102	26-27	-0.181991950611895
1102	28-29	-0.12554505620615686
1102	30-31	-0.08464575021834975
1102	32-33	-0.16481425093527236
1102	34-35	-0.17077142788433974
1102	36-37	-0.11002470168080691
1102	38-39	-0.10470338516450539
1102	40-41	-0.07204823281215766
1102	42-43	-0.09761620657981496
1102	44-45	-0.11899269613231667
1102	46-47	-0.054077077432062026
1102	48-49	-0.07561467080281403
1102	50-51	-0.0905944796956959
1102	52-53	-0.06501142637837631
1102	54-55	-0.11723185571886319
1102	56-57	-0.09446734207708118
1102	58-59	-0.0947924889596905
1102	60-61	-0.08038234792589094
1102	62-63	-0.0745510517503476
1102	64-65	-0.02896312357240305
1102	66-67	-0.1186140648866214
1102	68-69	-0.06265939335815318
1102	70-71	-0.09283084435856637
1102	72-73	-0.09460926446143958
1102	74-75	-0.166833291854239
1102	76-77	-0.16029358550802897
1102	78-79	-0.07788824108816073
1102	80-81	-0.00590456942067874
1102	82-83	-0.055151524907429916
1102	84-85	-0.11797058042775888
1102	86-87	-0.1250013511152872
1102	88-89	-0.035171825168333726
1102	90-91	-0.12701034380610565
1102	92-93	-0.043901130300923796
1102	94-95	-0.13883877660097
1102	96-97	-0.17173741495171413
1102	98-99	-0.2427360652213295
1102	100	-0.13817811922748646
1103	1	0.12267932251027247
1103	2	0.0789038478811861
1103	3	0.07084804814613932
1103	4	0.019690763409698775
1103	5	0.012903278771453586
1103	6	0.052561218770662776
1103	7	0.009565672464951547
1103	8	-0.005171078787761019
1103	9	-0.021525202174871083
1103	10-11	-0.03907783972983481
1103	12-13	-0.030064401859632994
1103	14-15	-0.025333956470468877
1103	16-17	-0.03418206641264021
1103	18-19	-0.03453204909578034
1103	20-21	-0.005366088952037273
1103	22-23	-0.08758862252043542
1103	24-25	-0.07387126742843009
1103	26-27	-0.09064961194997778
1103	28-29	-0.07202401276567372
1103	30-31	-0.1585313042443275
1103	32-33	-0.14893613190344723
1103	34-35	-0.15117314460751174
1103	36-37	-0.12810947025187147
1103	38-39	-0.11226367641719293
1103	40-41	-0.12363977276122995
1103	42-43	-0.1785042534817478
1103	44-45	-0.16949722900456976
1103	46-47	-0.07462211654178219
1103	48-49	-0.04513399982167954
1103	50-51	-0.07006553351717315
1103	52-53	-0.11721659548781815
1103	54-55	-0.14173060136928228
1103	56-57	-0.07341212408349662
1103	58-59	-0.09745054971413225
1103	60-61	-0.12117019670196782
1103	62-63	-0.13362610438652212
1103	64-65	-0.1744311743390341
1103	66-67	-0.1889239014615569
1103	68-69	-0.15411893770703244
1103	70-71	-0.14158472404361788
1103	72-73	-0.1449252373279748
1103	74-75	-0.13492769220943757
1103	76-77	-0.22298289471910948
1103	78-79	-0.19131871219329355
1103	80-81	-0.12358408382052488
1103	82-83	-0.1104794119373338
1103	84-85	-0.12070925567924462
1103	86-87	-0.09546803533134351
1103	88-89	-0.11340870400516678
1103	90-91	-0.07830666088035798
1103	92-93	-0.15899133103042118
1103	94-95	-0.12330202550064939
1103	96-97	-0.10410344025063978
1103	98-99	-0.07451090155891649
1103	100	0.04384228265346124
1104	1	0.15280316363097768
1104	2	0.08638716924628653
1104	3	0.07555828319152624
1104	4	0.03978987241155352
1104	5	0.02113678768343874
1104	6	0.066891004425635
1104	7	0.07711013210927575
1104	8	0.05145704883237556
1104	9	0.09969276943032668
1104	10-11	0.05966409174234144
1104	12-13	0.03719221844518472
1104	14-15	0.06305503583108418
1104	16-17	0.008817069066417105
1104	18-19	0.04122009415393535
1104	20-21	-0.015231356070962931
1104	22-23	-0.03491728289409224
1104	24-25	0.03914528884967439
1104	26-27	0.0676113770940816
1104	28-29	2.0987124008797764E-4
1104	30-31	0.03612029983316489
1104	32-33	0.003422144831517926
1104	34-35	-0.02319621387750459
1104	36-37	0.09755882850731723
1104	38-39	0.08733761093672854
1104	40-41	0.126205275399208
1104	42-43	0.08686055028604045
1104	44-45	0.02932904842040074
1104	46-47	0.08977050233485784
1104	48-49	0.11566789851386972
1104	50-51	0.06820165360046815
1104	52-53	0.02174311101634885
1104	54-55	0.00996549444611361
1104	56-57	0.11614537558553906
1104	58-59	0.12910909821923866
1104	60-61	0.05884096584802734
1104	62-63	0.039547118732095043
1104	64-65	0.04277269213560686
1104	66-67	0.07818072563105005
1104	68-69	0.02984675625656763
1104	70-71	0.07971985009456972
1104	72-73	0.022984888599225428
1104	74-75	-0.011494761784327068
1104	76-77	-0.043726182145242376
1104	78-79	0.070558421528645
1104	80-81	0.015282601433675325
1104	82-83	0.061425613115723365
1104	84-85	0.023992193747282897
1104	86-87	0.04059873537202918
1104	88-89	0.05049273258392617
1104	90-91	0.1048459120222276
1104	92-93	0.022667636518953316
1104	94-95	0.025470545315847914
1104	96-97	-3.621807379019515E-4
1104	98-99	0.08932258689340955
1104	100	0.12398447729303186
1105	1	0.10698675839561389
1105	2	0.08961175346858141
1105	3	0.03600131971461451
1105	4	0.033720601248198534
1105	5	0.012795176187999857
1105	6	0.038255888025453544
1105	7	0.049590529311899445
1105	8	0.047295431999963
1105	9	0.03363278425037919
1105	10-11	0.017982136111513114
1105	12-13	0.03456460298172459
1105	14-15	0.08395539386732054
1105	16-17	0.04990242586253402
1105	18-19	-0.014274754386256916
1105	20-21	-0.0031647565884327378
1105	22-23	-0.00886836525213397
1105	24-25	-0.021259133513353845
1105	26-27	0.02125651029975728
1105	28-29	0.004787859872642741
1105	30-31	-0.04371656244317279
1105	32-33	0.012576109709378613
1105	34-35	0.012569545193635179
1105	36-37	-0.06539470254129753
1105	38-39	0.015583176515654884
1105	40-41	0.008494293686574395
1105	42-43	0.014735348311170071
1105	44-45	0.036628406087103826
1105	46-47	0.042632032661252595
1105	48-49	-0.04867207856799638
1105	50-51	0.03820367752935283
1105	52-53	-0.04956822828566487
1105	54-55	-0.021785152543785102
1105	56-57	-0.04590116069204697
1105	58-59	-0.047095632944611054
1105	60-61	-0.021894871244249714
1105	62-63	-0.020650353277453348
1105	64-65	0.02888115335688468
1105	66-67	0.047566751904007276
1105	68-69	-0.01612387774574131
1105	70-71	-1.5478883358355233E-4
1105	72-73	0.10820439846869334
1105	74-75	0.062078397207621094
1105	76-77	0.046948288085673084
1105	78-79	0.1129491073954938
1105	80-81	0.04574907901288583
1105	82-83	0.04697918814682822
1105	84-85	0.059504791543012914
1105	86-87	0.0446656516279873
1105	88-89	0.0800412064501046
1105	90-91	0.0682424523674996
1105	92-93	0.041924184908452844
1105	94-95	0.05814455252014028
1105	96-97	0.01884124654699093
1105	98-99	-0.019725289663675483
1105	100	0.03934853013204531
1106	1	0.09898741959557356
1106	2	0.04169784198531801
1106	3	-0.027385410978489233
1106	4	0.011495154090134463
1106	5	0.008214005037572747
1106	6	-0.02481108058684356
1106	7	0.001619432490954864
1106	8	-0.021022807135722132
1106	9	-0.004435380495912966
1106	10-11	-0.00803847017113668
1106	12-13	-0.011718072513161815
1106	14-15	-8.443380576821369E-4
1106	16-17	-0.038994692780654816
1106	18-19	-0.009044719096117149
1106	20-21	-0.012049146427486335
1106	22-23	-0.03702029286060338
1106	24-25	-0.011564588686177046
1106	26-27	-0.02184735651285763
1106	28-29	-0.031740221784843925
1106	30-31	-0.04835923646832896
1106	32-33	-0.07965560140192451
1106	34-35	-0.05701395258058284
1106	36-37	-0.011164366521484226
1106	38-39	-0.007473264355283504
1106	40-41	0.007272078249052072
1106	42-43	-0.07027426878233456
1106	44-45	0.04282261789119701
1106	46-47	-0.002015397703708288
1106	48-49	-0.08391939369877122
1106	50-51	-0.0346155058693185
1106	52-53	-0.07664354719084088
1106	54-55	-0.056735835256539247
1106	56-57	0.012433214577519891
1106	58-59	-0.020642039374820342
1106	60-61	-0.01465246717970814
1106	62-63	-0.05186063909575722
1106	64-65	0.0068261167428502745
1106	66-67	-0.0032038271297025744
1106	68-69	0.057066173274328946
1106	70-71	0.014438178004290592
1106	72-73	-0.04437965186215109
1106	74-75	-0.07357879317012817
1106	76-77	-0.06981425874308655
1106	78-79	-0.04137557121453739
1106	80-81	0.06975700812562025
1106	82-83	0.0677325313964019
1106	84-85	0.04267547268207039
1106	86-87	0.05602346151942328
1106	88-89	0.020844953860787996
1106	90-91	0.09517339812046544
1106	92-93	0.023335557812096397
1106	94-95	0.010768967416765918
1106	96-97	0.0458799985567353
1106	98-99	0.06840277870637834
1106	100	0.04557811930606093
1107	1	0.06136261525832509
1107	2	0.02309326215650742
1107	3	-0.007223111381215119
1107	4	-0.026840916173803464
1107	5	-0.029872971368817502
1107	6	-0.01074860791629817
1107	7	-0.06401305093344689
1107	8	-0.016538686890683607
1107	9	-0.09737168699836474
1107	10-11	-0.06100628433247124
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2316	4	-0.16455314539926036
2316	5	-0.06491935070912547
2316	6	-0.06228025988353636
2316	7	-0.0810187176879964
2316	8	-0.028919588037283006
2316	9	-0.05766146746471179
2316	10-11	-0.05762136027851028
2316	12-13	-0.033580077328437596
2316	14-15	-0.007682877436373303
2316	16-17	-0.05204179198045722
2316	18-19	-0.026798943930906205
2316	20-21	-0.03251288671464181
2316	22-23	-0.039888212230145825
2316	24-25	-0.02063351595375451
2316	26-27	-0.03824860171975075
2316	28-29	-0.04301300531739827
2316	30-31	-0.34204755760178074
2316	32-33	-0.21099636982328462
2316	34-35	-0.2918551718664091
2316	36-37	-0.4728539003312804
2316	38-39	-0.3653651318451665
2316	40-41	-0.3355114412545035
2316	42-43	-0.37098922145948166
2316	44-45	-0.25963638948456236
2316	46-47	-0.36996084602115076
2316	48-49	-0.377441093037568
2316	50-51	-0.40936694171365673
2316	52-53	-0.45303367042623677
2316	54-55	-0.3429934503453751
2316	56-57	-1.060835689530819
2316	58-59	-0.8066616157175304
2316	60-61	-0.48374690796006803
2316	62-63	-0.2674991826939461
2316	64-65	-0.22064473196837042
2316	66-67	-0.1632167834060354
2316	68-69	-0.3086936792482007
2316	70-71	-0.3534684584898429
2316	72-73	-0.2089417027907743
2316	74-75	-0.17335340223024076
2316	76-77	-0.15694416489917984
2316	78-79	-0.27106167733032294
2316	80-81	-0.21747990624971436
2316	82-83	-0.40186000332919747
2316	84-85	-0.18814095786247265
2316	86-87	-0.279346347096201
2316	88-89	-0.3016254109679437
2316	90-91	-0.23281058977813984
2316	92-93	-0.23240741329877324
2316	94-95	-0.15774199340707895
2316	96-97	-0.36027363867184903
2316	98-99	-0.1792331430559173
2316	100	-0.5819438800318757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	3.0
12	9.0
13	36.0
14	189.0
15	564.0
16	1299.0
17	2673.0
18	4988.0
19	7852.0
20	12000.0
21	18036.0
22	24428.0
23	26768.0
24	26778.0
25	29200.0
26	34620.0
27	43067.0
28	53644.0
29	66714.0
30	82517.0
31	103777.0
32	131246.0
33	173444.0
34	254616.0
35	393597.0
36	719202.0
37	1499414.0
38	1765375.0
39	333098.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66576606613195	19.889112119584087	13.792852663710347	23.652269150573616
2	15.74209301028513	20.626306986685176	39.13809284778326	24.493507155246437
3	18.80476447135098	25.72635843776322	30.068422182441594	25.40045490844421
4	13.24748997608583	17.43195340833739	38.716477641662024	30.604078973914756
5	14.000758145154155	37.70190090609019	34.17993449377973	14.117406454975923
6	30.293651752721633	37.19293933030985	18.401829926836665	14.111578990131848
7	27.56916968831466	32.43244499407146	22.108255330517565	17.890129987096312
8	26.69720108614259	34.15955981410119	20.068780768101256	19.074458331654956
9	26.262653562484378	16.137204176390025	20.65964128988011	36.94050097124549
10-11	25.29207159963038	25.860145438470617	27.838294895400256	21.00948806649875
12-13	26.09767866128003	23.318462071569	27.809293927502125	22.77456533964884
14-15	23.587898322275542	24.82386492546794	25.363097842758474	26.225138909498042
16-17	23.045994626272737	27.68041910149428	26.033614499922926	23.23997177231006
18-19	22.639672054417055	27.178102004696225	27.81966057077866	22.362565370108058
20-21	23.900923425813627	26.245667743151856	27.51625938481716	22.337149446217357
22-23	23.826372756019545	25.975307532620135	27.22100819556218	22.977311515798142
24-25	23.484763979305907	26.177607427123277	27.49525149271999	22.842377100850825
26-27	23.32134232707577	26.492750310032093	27.064588240883747	23.121319122008387
28-29	23.052300225331603	26.44501412185364	27.434959892317366	23.06772576049739
30-31	23.5748924748814	26.151320758517205	27.349876480280617	22.923910286320783
32-33	23.52886438099721	26.433082830259767	26.9437933495553	23.094259439187717
34-35	23.630674367851423	26.451811887093555	27.220274755625056	22.697238989429966
36-37	23.59486522838543	26.215079356539515	26.988273524506372	23.201781890568675
38-39	23.520822869066357	26.301070464921196	27.20068107582779	22.97742559018466
40-41	23.59629718804493	25.812562899244224	27.490590561439802	23.100549351271045
42-43	23.36583584294506	25.930851693373548	27.22816896025682	23.47514350342457
44-45	23.481809114567824	26.276587943298797	27.090024681888025	23.15157826024536
46-47	23.429900627798737	26.168248271436017	26.736061139623075	23.66578996114217
48-49	23.769870957728706	26.061043354887293	26.92701515405456	23.242070533329443
50-51	23.640799554483273	26.211196964702093	26.682983343906034	23.4650201369086
52-53	23.394426789421676	26.32922811016275	26.659224012973286	23.617121087442285
54-55	23.387536242986496	26.226008186600435	26.687895649464423	23.69855992094865
56-57	23.746230752241754	26.19234925117697	26.466880110335655	23.59453988624562
58-59	23.23930411874229	26.311241296492156	26.909202926692227	23.54025165807333
60-61	23.388696095027008	26.474541670715578	26.64005586340698	23.49670637085043
62-63	23.441164888935752	26.198217893792325	26.51792111995972	23.842696097312206
64-65	23.076613222173517	26.1729486991566	26.743752790306573	24.00668528836331
66-67	23.37650969510012	26.13896769218057	26.599674305230398	23.884848307488916
68-69	23.095999236370982	26.136261817335498	26.750298045128652	24.017440901164868
70-71	23.159511063810193	25.897204277567642	26.746684213720012	24.196600444902153
72-73	23.222105880616297	26.113795862226862	26.695836706307453	23.968261550849387
74-75	23.273700349506967	26.304109280578935	26.56956207820128	23.85262829171282
76-77	23.103447593210838	26.303820738683775	26.36478653921948	24.227945128885903
78-79	23.080528825463496	26.170559983474206	26.491082956051713	24.257828235010585
80-81	22.974532599011752	26.5338009043114	26.510441290982715	23.981225205694134
82-83	23.33807324213463	26.113652632938862	26.851561841694277	23.69671228323223
84-85	23.39880148583875	26.174123589854865	26.467636735027977	23.95943818927841
86-87	22.81825597508253	26.436560289478116	26.748123344092328	23.997060391347024
88-89	23.067740543897806	25.93522135764092	26.757000130484794	24.240037967976484
90-91	23.093285092499613	26.07168835085601	26.649850198474432	24.185176358169944
92-93	23.03485233462097	26.14879636706673	26.694450756402045	24.12190054191025
94-95	22.706405435424042	25.990445177865258	26.920725136357284	24.382424250353417
96-97	22.763729333988973	26.014593863354225	26.83223546976542	24.38944133289138
98-99	22.57709232142679	26.210614634965918	26.700116059805744	24.51217698380155
100	22.833630283781343	25.919831986297243	26.504178824420517	24.7423589055009
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	69.5
2	110.5
3	188.5
4	147.0
5	119.5
6	265.0
7	515.0
8	814.0
9	1051.5
10	1034.0
11	955.5
12	1042.0
13	1258.0
14	1490.0
15	1724.5
16	2046.0
17	2338.5
18	2689.5
19	3077.0
20	3517.5
21	4034.5
22	4613.5
23	5348.5
24	6296.5
25	7519.0
26	9317.5
27	11604.5
28	14432.0
29	18870.0
30	25301.5
31	33142.5
32	44024.5
33	58689.5
34	73979.0
35	91028.5
36	114897.5
37	143302.0
38	163745.5
39	176302.0
40	182982.5
41	185738.5
42	193764.0
43	207281.0
44	222563.5
45	243606.5
46	260779.5
47	271988.5
48	281327.0
49	279374.0
50	270163.5
51	261982.0
52	253324.5
53	243625.5
54	233425.0
55	211648.5
56	185839.0
57	157607.0
58	131568.5
59	116283.5
60	96275.5
61	74770.5
62	58775.5
63	45148.0
64	32530.5
65	21475.0
66	14725.0
67	10831.0
68	7970.5
69	5760.5
70	4286.5
71	3295.5
72	2463.5
73	1757.0
74	1216.5
75	820.5
76	549.5
77	309.0
78	162.5
79	93.0
80	58.5
81	35.5
82	18.0
83	14.0
84	11.0
85	10.0
86	5.0
87	2.0
88	2.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3522691788223978
2	5.164227601970256E-4
3	0.00194519239674213
4	0.0014804119125648068
5	0.004819945761838905
6	0.0
7	3.442818401313504E-5
8	2.0656910407881025E-4
9	0.0027198265370376685
10-11	3.442818401313504E-5
12-13	5.164227601970256E-5
14-15	2.7542547210508033E-4
16-17	7.746341402955385E-5
18-19	0.0010242384743907676
20-21	1.8074796606895897E-4
22-23	2.3239024208866155E-4
24-25	0.0
26-27	0.002031262856774967
28-29	0.011077268206226199
30-31	0.003485853631329923
32-33	0.014038092031355812
34-35	0.011197766850272172
36-37	0.01302246060296833
38-39	0.01267817876283698
40-41	0.013728238375237598
42-43	0.006205680168367591
44-45	0.0013082709924991315
46-47	0.005611793994141012
48-49	0.012884747866915788
50-51	0.003597745229372612
52-53	0.005749506730193552
54-55	0.005362189660045783
56-57	1.8935501207224272E-4
58-59	7.229918642758359E-4
60-61	4.2174525416090425E-4
62-63	9.72596198371065E-4
64-65	3.5288888613463414E-4
66-67	0.0012738428084859965
68-69	0.0015578753265943607
70-71	8.865257383382272E-4
72-73	0.0026079349389949795
74-75	0.00757420048288971
76-77	0.00343421135531022
78-79	0.0016181246486173468
80-81	3.873170701477692E-4
82-83	1.8074796606895897E-4
84-85	0.0014545907745549555
86-87	0.004432628691691137
88-89	0.0015148400965779418
90-91	1.291056900492564E-4
92-93	0.001962406488748697
94-95	0.003270677481247829
96-97	0.001867728982712576
98-99	0.002065691040788102
100	0.002478829248945723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5809194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.09538007997584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23597735848325	45.96570632404991
2	14.451672005392528	17.658607879212063
3	4.757537024675467	8.71990598301315
4	2.068302648933226	5.054549458279851
5	1.102607937024817	3.3682125495864628
6	0.623921869944757	2.287124627069051
7	0.3988224834740383	1.7056347848600373
8	0.25760882222257275	1.2590967124514407
9	0.18327882175728943	1.0077740348284567
>10	0.8671743867432034	9.009999600049403
>50	0.037889003755383496	1.6017524218171222
>100	0.014298041364263069	1.5734391150522096
>500	7.074643520543325E-4	0.28008357972020276
>1k	1.3475458470292486E-4	0.17361091119370292
>5k	6.737729235146072E-5	0.3345020188168363
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8689	0.14957324544506517	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7563	0.13019017784567016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.442818401313504E-5	0.0
6	0.0	0.0	0.0	3.442818401313504E-5	0.0
7	0.0	0.0	0.0	3.442818401313504E-5	0.0
8	0.0	0.0	0.0	5.164227601970256E-5	0.0
9	0.0	0.0	0.0	1.549268280591077E-4	1.721409200656752E-5
10-11	0.0	0.0	0.0	1.9796205807552647E-4	1.721409200656752E-5
12-13	0.0	0.0	0.0	2.2378319608537777E-4	2.582113800985128E-5
14-15	0.0	0.0	0.0	2.496043340952291E-4	6.885636802627008E-5
16-17	0.0	0.0	0.0	2.9263956411164783E-4	1.0328455203940512E-4
18-19	0.0	0.0	0.0	2.9263956411164783E-4	1.0328455203940512E-4
20-21	0.0	0.0	0.0	4.561734381740393E-4	1.2049864404597264E-4
22-23	0.0	0.0	0.0	6.369214042429983E-4	1.5492682805910767E-4
24-25	0.0	0.0	0.0	0.0010844877964137538	1.721409200656752E-4
26-27	0.0	0.0	0.0	0.0018246937526961572	1.8935501207224272E-4
28-29	0.0	0.0	0.0	0.0033567479412806665	1.8935501207224272E-4
30-31	0.0	0.0	0.0	0.008934113751408543	1.9796205807552647E-4
32-33	0.0	0.0	0.0	0.02318738193284645	2.7542547210508033E-4
34-35	0.0	0.0	0.0	0.04788960396227084	3.6149593213791794E-4
36-37	0.0	0.0	0.0	0.08805008061359287	3.6149593213791794E-4
38-39	0.0	0.0	0.0	0.14727516416218844	3.6149593213791794E-4
40-41	0.0	0.0	0.0	0.21411748342369008	3.6149593213791794E-4
42-43	0.0	0.0	0.0	0.28326649101407186	3.701029781412017E-4
44-45	0.0	0.0	0.0	0.37095507569552677	3.873170701477692E-4
46-47	0.0	0.0	0.0	0.4788443973466887	4.131382081576205E-4
48-49	0.0	0.0	0.0	0.5984306945163133	4.30352300164188E-4
50-51	0.0	0.0	0.0	0.7236718209100952	4.30352300164188E-4
52-53	0.0	0.0	0.0	0.8485686654637459	4.733875301806068E-4
54-55	0.0	0.0	0.0	0.9894229733074846	5.164227601970257E-4
56-57	0.0	0.0	0.0	1.156408272817193	5.250298062003094E-4
58-59	0.0	0.0	0.0	1.3479064393442532	5.422438982068769E-4
60-61	0.0	0.0	0.0	1.5482870773467026	5.852791282232957E-4
62-63	0.0	0.0	0.0	1.7529023819827674	6.197073122364308E-4
64-65	0.0	0.0	0.0	1.964067648627331	6.45528450246282E-4
66-67	0.0	0.0	0.0	2.2007786278096413	6.79956634259417E-4
68-69	0.0	0.0	0.0	2.4701791677124225	6.885636802627008E-4
70-71	0.0	1.721409200656752E-5	0.0	2.7642905366906323	6.885636802627008E-4
72-73	0.0	1.721409200656752E-5	0.0	3.0644526590091496	7.229918642758359E-4
74-75	0.0	1.721409200656752E-5	0.0	3.3651312040878647	7.229918642758359E-4
76-77	0.0	1.721409200656752E-5	0.0	3.691725564682467	7.229918642758359E-4
78-79	0.0	1.721409200656752E-5	0.0	4.058420152606368	7.229918642758359E-4
80-81	0.0	1.721409200656752E-5	0.0	4.446537678032444	7.488130022856871E-4
82-83	0.0	1.721409200656752E-5	0.0	4.854064092195923	8.090623243086735E-4
84-85	0.0	1.721409200656752E-5	0.0	5.260721194713071	8.090623243086735E-4
86-87	0.0	1.721409200656752E-5	0.0	5.686038372965337	8.176693703119572E-4
88	0.0	1.721409200656752E-5	0.0	6.021678738909391	8.60704600328376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8295	0.0	49.643024	1
GTATCAA	14970	0.0	40.300377	1
CAACGCA	16485	0.0	34.954254	5
ATCAACG	16920	0.0	34.084557	3
TATCAAC	18430	0.0	32.437626	2
TCAACGC	18345	0.0	31.615747	4
AACGCAG	18665	0.0	31.598442	6
GTACTAG	2710	0.0	28.023151	1
ACGCAGA	20695	0.0	27.68141	7
CGCAGAG	21525	0.0	26.439587	8
GTACATG	23775	0.0	23.907099	1
GCAGAGT	23850	0.0	23.389235	9
TACATGG	24190	0.0	22.926273	2
ACATGGG	24100	0.0	22.135653	3
GTGGTAT	3630	0.0	21.18075	1
CATGGGG	18265	0.0	20.766336	4
GAGTACT	9775	0.0	20.720184	12-13
CAGAGTA	23535	0.0	19.587734	10-11
GTGATCG	1955	0.0	19.23078	8
GTATAAG	3580	0.0	19.104923	1
>>END_MODULE