FastQCFastQC Report
Thu 26 May 2016
ERR580183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440772
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287280.25110193612808646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228620.1998291723670396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133220.11644319107137176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161700.049.927311
GTATCAA292200.042.3851241
CAACGCA354300.033.8768045
ATCAACG354300.033.839223
TATCAAC373250.033.0177152
AACGCAG386950.031.4908476
TCAACGC382550.031.486834
ACGCAGA428800.027.9021997
GTGGTAT68200.027.3016381
GTACTAG59850.027.0254041
CGCAGAG444400.026.8907748
GTACATG418600.026.5313011
TACATGG427250.025.6310982
ACATGGG426100.024.9261953
GCAGAGT491550.023.7311219
CATGGGG329350.022.9115984
TAGTACT79350.021.8041364
GCATCGT63200.021.6374918
ATGGGGG228100.021.2704755
TAACGCC28250.021.1360234
Produced by FastQC (version 0.11.5)