FastQCFastQC Report
Thu 26 May 2016
ERR580183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11440772
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258950.2263396211374547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215260.1881516387180865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129510.11320040290987356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163350.049.2872921
GTATCAA294350.041.997511
ATCAACG355300.033.597683
CAACGCA357050.033.5112535
TATCAAC381050.032.1780052
TCAACGC387700.031.1411974
AACGCAG393700.031.0709726
GTACTAG59200.027.4067361
GTGGTAT66900.027.3543361
ACGCAGA438350.027.2628637
CGCAGAG455900.026.1105218
GTACATG422700.025.6858351
TACATGG427500.024.7790572
ACATGGG429400.023.7612113
GCAGAGT500600.023.3752639
CATGGGG327350.022.152394
TAGTACT79950.021.6319854
TGGTATC81200.021.1253472
GCATCGT69350.020.7359948
TAGGCAT81400.020.553555
Produced by FastQC (version 0.11.5)