FastQCFastQC Report
Thu 26 May 2016
ERR580182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8494223
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304750.3587732509494983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243490.2866536468373858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136490.16068568013813622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143350.050.9079061
GTATCAA262750.041.840131
ATCAACG310150.034.6114853
CAACGCA311950.034.3331685
TATCAAC326200.033.269842
TCAACGC325600.032.9824144
AACGCAG333800.032.3780946
ACGCAGA371800.028.7271277
CGCAGAG378250.028.1499738
GTGGTAT58250.027.8493061
GCAGAGT413900.025.4355819
GTACATG293950.025.1300851
TACATGG297350.024.2422622
TGGTATC66950.023.8796122
ACATGGG302000.023.0742363
GTACTAG34000.022.6806851
CATGGGG240050.022.2900644
GAGTACT255900.022.23926712-13
ATGGGGG174850.021.161885
CAGAGTA410300.021.14195310-11
Produced by FastQC (version 0.11.5)