FastQCFastQC Report
Thu 26 May 2016
ERR580182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8494223
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267980.31548500669219537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230500.27136090022595355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128240.15097319672440904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143250.054.4283261
GTATCAA254850.040.976961
ATCAACG307050.033.2441023
CAACGCA309100.033.1292725
TATCAAC322450.032.0351372
TCAACGC325800.031.5037964
AACGCAG330250.031.3334126
GTGGTAT55850.029.1368561
ACGCAGA366300.027.9292837
CGCAGAG374950.027.2099238
TGGTATC58650.026.8434352
GTACATG294250.025.5516261
TACATGG296200.024.9418582
GCAGAGT409400.024.4843739
ACATGGG297350.024.1659243
CATGGGG233800.022.191464
GAGTACT255100.022.05145812-13
CAGAGTA403350.021.45522510-11
ATGGGGG170650.021.2325275
AGAGTAC380600.020.63246310-11
Produced by FastQC (version 0.11.5)