##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580182_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8494223
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.519861910854	34.0	31.0	34.0	31.0	34.0
2	32.714723524447145	34.0	31.0	34.0	31.0	34.0
3	32.79272618578533	34.0	31.0	34.0	31.0	34.0
4	36.27075437035265	37.0	37.0	37.0	35.0	37.0
5	36.20493551911694	37.0	37.0	37.0	35.0	37.0
6	36.23697917984965	37.0	37.0	37.0	35.0	37.0
7	36.20612362072434	37.0	37.0	37.0	35.0	37.0
8	36.19596695306916	37.0	37.0	37.0	35.0	37.0
9	37.98698750903997	39.0	38.0	39.0	35.0	39.0
10-11	37.93628687403192	39.0	38.0	39.0	35.0	39.0
12-13	37.896497125163776	39.0	38.0	39.0	35.0	39.0
14-15	39.28776010472059	41.0	39.0	41.0	36.0	41.0
16-17	39.270582900872746	41.0	39.0	41.0	36.0	41.0
18-19	39.28293458977943	41.0	39.0	41.0	36.0	41.0
20-21	39.274700876113094	41.0	39.0	41.0	36.0	41.0
22-23	39.202369775316704	41.0	39.0	41.0	36.0	41.0
24-25	39.12087827220924	40.5	39.0	41.0	35.0	41.0
26-27	38.97952408360364	40.0	38.5	41.0	35.0	41.0
28-29	38.862375640479414	40.0	38.0	41.0	35.0	41.0
30-31	38.73075230071073	40.0	38.0	41.0	35.0	41.0
32-33	38.55709645249483	40.0	38.0	41.0	34.5	41.0
34-35	38.51126600985164	40.0	38.0	41.0	34.5	41.0
36-37	38.5449251803255	40.0	38.0	41.0	35.0	41.0
38-39	38.444595638706446	40.0	38.0	41.0	34.0	41.0
40-41	38.315446686530365	40.0	38.0	41.0	34.0	41.0
42-43	38.27701132875838	40.0	38.0	41.0	34.0	41.0
44-45	38.179515889799454	40.0	38.0	41.0	33.5	41.0
46-47	37.966708903215746	40.0	38.0	41.0	33.0	41.0
48-49	37.74248851248666	40.0	37.0	41.0	33.0	41.0
50-51	37.57419530897647	40.0	37.0	41.0	33.0	41.0
52-53	37.41078483576426	40.0	36.5	41.0	32.0	41.0
54-55	37.22409383412703	40.0	36.0	41.0	32.0	41.0
56-57	37.028125115151795	39.0	36.0	41.0	31.5	41.0
58-59	36.814970539388945	39.0	35.0	41.0	31.0	41.0
60-61	36.56350151155674	39.0	35.0	41.0	31.0	41.0
62-63	36.283222903377975	38.0	35.0	40.0	31.0	41.0
64-65	35.99841657088588	37.5	35.0	40.0	30.0	41.0
66-67	35.67845552206482	37.0	35.0	40.0	30.0	41.0
68-69	35.32617692047877	36.5	35.0	39.5	29.5	41.0
70-71	34.990802219343664	36.0	34.0	39.0	29.0	41.0
72-73	34.6281704636198	35.5	34.0	39.0	29.0	40.0
74-75	34.267153511274664	35.0	34.0	37.5	29.0	40.0
76-77	33.01953333459694	34.5	32.5	36.0	26.5	39.0
78-79	33.64002775768896	35.0	33.5	37.0	28.5	39.0
80-81	33.56547938522452	35.0	34.0	36.0	29.0	38.5
82-83	33.33878925712216	35.0	34.0	36.0	29.0	37.0
84-85	33.099262581168404	35.0	34.0	35.5	28.5	37.0
86-87	32.90497053114805	35.0	33.0	35.0	28.0	36.5
88-89	32.730582185092146	35.0	33.0	35.0	27.0	36.0
90-91	32.56100528559234	35.0	33.0	35.0	27.0	36.0
92-93	32.430351310531876	35.0	33.0	35.0	27.0	36.0
94-95	32.321877351230356	35.0	33.0	35.0	26.5	35.0
96-97	32.21520090772282	35.0	33.0	35.0	26.0	35.0
98-99	32.13650718847386	35.0	33.0	35.0	25.5	35.0
100	30.877697583404625	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.17170197683511645
1101	2	0.10238257555850794
1101	3	0.049598967447543885
1101	4	0.06471614391603708
1101	5	0.0483984083328437
1101	6	0.06491276777929045
1101	7	0.028226994055351895
1101	8	0.07743313574749777
1101	9	0.06273089443330093
1101	10-11	0.033007109389814104
1101	12-13	0.017447180882726343
1101	14-15	0.06576150744396614
1101	16-17	0.013501613076698504
1101	18-19	0.023402932864406978
1101	20-21	-0.0172586586625485
1101	22-23	-0.005139137184364984
1101	24-25	-0.023433035321204443
1101	26-27	-0.016922658894415576
1101	28-29	0.0042865090150243645
1101	30-31	-0.002675695617888607
1101	32-33	-0.04447842112384848
1101	34-35	-0.1118426152307066
1101	36-37	-0.03106099682209873
1101	38-39	0.02971797859976988
1101	40-41	0.10128743048909428
1101	42-43	0.0515756199294799
1101	44-45	0.05290767509056593
1101	46-47	0.06254146235179547
1101	48-49	0.022952887760226304
1101	50-51	0.014850769449218149
1101	52-53	-0.02546374192655776
1101	54-55	-0.018208074606306468
1101	56-57	-0.01138228406114905
1101	58-59	0.006426606093988596
1101	60-61	-0.06569004117046973
1101	62-63	-0.021376220122178324
1101	64-65	0.0037678247802332976
1101	66-67	-0.02438639158618372
1101	68-69	-0.06242768045036229
1101	70-71	-0.10221946861344122
1101	72-73	-0.022791729093469826
1101	74-75	-0.06995685563178
1101	76-77	-0.10833526112261183
1101	78-79	-0.03895261308801423
1101	80-81	4.40972231466219E-4
1101	82-83	0.012634646632626811
1101	84-85	0.018108330828255248
1101	86-87	-0.041233919276088216
1101	88-89	-0.011296242423945557
1101	90-91	-0.03191333605032298
1101	92-93	-0.13616778236488614
1101	94-95	-0.08086845465456349
1101	96-97	-0.045886631965366576
1101	98-99	-0.08051881661177163
1101	100	-0.014568540307017486
1102	1	0.13790438859773246
1102	2	0.038058098970907395
1102	3	0.04891776970172401
1102	4	0.04373729362657741
1102	5	0.018376890303379412
1102	6	0.05879582139106532
1102	7	0.047313776746754854
1102	8	0.00535970098545846
1102	9	0.00848669171774219
1102	10-11	0.025517726332566326
1102	12-13	0.028559212435098402
1102	14-15	0.06257834739572132
1102	16-17	-0.021670227787303986
1102	18-19	-0.009394356487717914
1102	20-21	-0.021321596762312822
1102	22-23	-0.02729972188852514
1102	24-25	-0.00929649966412427
1102	26-27	-0.0022544482364921237
1102	28-29	-0.05509901686280472
1102	30-31	-0.0244531132663397
1102	32-33	-0.05432985763177811
1102	34-35	0.0025327508983892244
1102	36-37	0.03341345649988625
1102	38-39	0.008523403228338111
1102	40-41	-0.00374470021029083
1102	42-43	0.0013974798289879686
1102	44-45	0.02712596409104151
1102	46-47	0.029497816985674774
1102	48-49	0.07823007659801817
1102	50-51	0.06495416152984745
1102	52-53	0.007682024349861649
1102	54-55	-0.021065564021412797
1102	56-57	0.02012850614532624
1102	58-59	0.026964659378883482
1102	60-61	0.013526115682125806
1102	62-63	0.04713406546171939
1102	64-65	0.028674671495885207
1102	66-67	0.04324983087161627
1102	68-69	0.07189344302492628
1102	70-71	-0.027528464482884374
1102	72-73	0.012226636647227451
1102	74-75	0.02838277693804514
1102	76-77	0.07820802297477059
1102	78-79	0.10023268637194604
1102	80-81	0.11068596948189935
1102	82-83	0.09751851400705647
1102	84-85	0.08575053526406862
1102	86-87	0.06069033350008368
1102	88-89	0.0976583300077678
1102	90-91	0.04043868797330674
1102	92-93	-0.03164478742777277
1102	94-95	0.008828207786258702
1102	96-97	0.005546441664669999
1102	98-99	-0.060052253305137526
1102	100	0.04286646016085882
1103	1	0.13584931207463313
1103	2	0.08413229803024791
1103	3	0.0686777797938376
1103	4	0.03572570931829944
1103	5	0.04540687298432289
1103	6	0.04795679812610132
1103	7	0.003372790410779203
1103	8	0.03822869853526356
1103	9	0.046536003898779654
1103	10-11	0.026722986404898563
1103	12-13	0.02519493475258372
1103	14-15	0.05018893189647855
1103	16-17	0.05861277638744866
1103	18-19	0.05416003617308007
1103	20-21	0.00785500616326118
1103	22-23	0.008353172971105494
1103	24-25	-8.781505765611541E-4
1103	26-27	-0.05551013286808626
1103	28-29	-0.03667447197862117
1103	30-31	-0.09116541010960333
1103	32-33	-0.07831757928092742
1103	34-35	-0.04944196445809723
1103	36-37	0.06878798130689745
1103	38-39	0.040271573980660946
1103	40-41	0.027470934817145576
1103	42-43	0.019531493435287928
1103	44-45	0.030570765614598372
1103	46-47	0.01610089579335039
1103	48-49	0.03847301091302313
1103	50-51	0.04694187824629381
1103	52-53	0.03605556660333775
1103	54-55	0.04876383720866073
1103	56-57	0.06348962374592304
1103	58-59	0.05319871807223109
1103	60-61	0.007756381752415109
1103	62-63	-0.027205615427732255
1103	64-65	0.10616672038973007
1103	66-67	0.11870314939842785
1103	68-69	0.07466464123437788
1103	70-71	0.10258230683108849
1103	72-73	0.08107542594018469
1103	74-75	-0.017352600152825914
1103	76-77	0.01750865832860171
1103	78-79	0.09259331164626161
1103	80-81	0.08917732737675266
1103	82-83	0.05146056927143405
1103	84-85	0.0920059048320283
1103	86-87	0.10917552498468552
1103	88-89	0.13546604442787924
1103	90-91	0.11647903815782001
1103	92-93	0.07296743662130467
1103	94-95	0.032965996593716795
1103	96-97	-0.00866858758419653
1103	98-99	0.09366074062427288
1103	100	0.11593267578906463
1104	1	0.10451740142673316
1104	2	0.07006939194913997
1104	3	0.02428281959660694
1104	4	0.03738881788847692
1104	5	0.006262608392731295
1104	6	0.04006014720533102
1104	7	0.002278835530844958
1104	8	0.0017536480679254396
1104	9	0.017222008860713345
1104	10-11	-0.013729093538096038
1104	12-13	-0.022085835608216087
1104	14-15	0.017679469489493727
1104	16-17	0.015870462185723966
1104	18-19	-0.02642684321894251
1104	20-21	-0.04759833797508861
1104	22-23	-0.030187127505222122
1104	24-25	-0.028500845137862996
1104	26-27	0.005103127510544425
1104	28-29	-0.020433989064549962
1104	30-31	-0.022098441256467538
1104	32-33	-0.05983943427700211
1104	34-35	-0.07973570765953042
1104	36-37	7.721252296093439E-4
1104	38-39	0.03676448524274889
1104	40-41	0.05876832026511636
1104	42-43	0.06110317258621478
1104	44-45	-0.0010409249111091867
1104	46-47	-0.008903659380884221
1104	48-49	0.05502687192331024
1104	50-51	0.0693493553129727
1104	52-53	0.05801700998021175
1104	54-55	-0.01646087750337699
1104	56-57	0.03840479715771039
1104	58-59	0.04499925534813798
1104	60-61	0.008730743051124534
1104	62-63	0.08671373686863149
1104	64-65	0.04251841878713236
1104	66-67	0.0036792435173111926
1104	68-69	-0.003198409912208433
1104	70-71	-0.007425857607287867
1104	72-73	-0.04913145844432165
1104	74-75	-0.09117396000075217
1104	76-77	-0.07257357154450972
1104	78-79	0.0657689361653766
1104	80-81	0.07290308418456704
1104	82-83	0.0612878958206764
1104	84-85	0.08135062172318897
1104	86-87	0.01828656837356135
1104	88-89	0.07587346849454235
1104	90-91	0.14055744155491823
1104	92-93	0.08501807973515696
1104	94-95	0.04637702074546013
1104	96-97	-0.014953664921549148
1104	98-99	0.0491443717771034
1104	100	0.105095380182334
1105	1	0.1264271985155574
1105	2	0.08194363782568104
1105	3	0.0011354912350824975
1105	4	0.009254732035806512
1105	5	-0.02972962060561457
1105	6	0.014624825582913559
1105	7	0.0037454166630652708
1105	8	0.007224155746911265
1105	9	-0.036273108314226477
1105	10-11	-0.0066109975723946945
1105	12-13	0.010469678412441397
1105	14-15	0.037354300728942746
1105	16-17	-0.019611068055297665
1105	18-19	-0.04283279740411672
1105	20-21	0.011170923193667193
1105	22-23	-0.01613208551938783
1105	24-25	-0.031621329816452715
1105	26-27	0.006712876163341264
1105	28-29	-1.223680612341127E-4
1105	30-31	-0.020709012736318755
1105	32-33	-0.034124813318953784
1105	34-35	-0.06251640255072033
1105	36-37	-0.034795189992806286
1105	38-39	0.0046977126069407404
1105	40-41	0.030414160918923017
1105	42-43	0.04295887340744997
1105	44-45	0.06265615348880971
1105	46-47	0.02411543234354241
1105	48-49	0.007488472515937872
1105	50-51	0.014325549380259872
1105	52-53	-0.021759329614553735
1105	54-55	-0.015925112422515042
1105	56-57	0.04278783399902153
1105	58-59	0.012527449347821573
1105	60-61	0.0034775698732971705
1105	62-63	0.01494991703923887
1105	64-65	0.03506626310991834
1105	66-67	-0.013853598331749595
1105	68-69	0.03567420057819248
1105	70-71	0.05604597436801839
1105	72-73	0.02807645271585102
1105	74-75	-0.023068927717574184
1105	76-77	-0.059771047403373245
1105	78-79	0.04674162349165556
1105	80-81	0.07850379135864927
1105	82-83	0.059007340676515696
1105	84-85	0.0952105526909719
1105	86-87	0.05480430096714173
1105	88-89	0.01643782735763466
1105	90-91	0.04187854324931095
1105	92-93	0.11929183766085316
1105	94-95	0.06596128999313322
1105	96-97	0.02914528700626562
1105	98-99	0.017931106808504182
1105	100	0.1322062901224612
1106	1	0.13677983072621203
1106	2	0.08471968210619707
1106	3	0.060264577546760734
1106	4	0.040430475387417175
1106	5	0.05161504933339245
1106	6	0.0436262736361428
1106	7	0.020551128466578916
1106	8	0.049984402659113414
1106	9	0.01459765145818892
1106	10-11	0.022736605786022324
1106	12-13	-8.904227616071125E-4
1106	14-15	0.049305190207377336
1106	16-17	0.029200405799826967
1106	18-19	0.024303433248341832
1106	20-21	-0.00222098446798924
1106	22-23	0.023891470609541443
1106	24-25	0.014573865240073758
1106	26-27	-0.018676115180674913
1106	28-29	-0.015136689236143752
1106	30-31	-0.0348656803109364
1106	32-33	0.009202143387462058
1106	34-35	0.04948903378997471
1106	36-37	0.03203629883724801
1106	38-39	-0.0039123273116956625
1106	40-41	0.0110109433925345
1106	42-43	0.039200410364991
1106	44-45	-0.012109311327876071
1106	46-47	0.0065839254478063935
1106	48-49	0.007863059508274262
1106	50-51	0.007071912767308675
1106	52-53	-0.04023782740780035
1106	54-55	-0.04271866409254699
1106	56-57	-0.05469211962098797
1106	58-59	-0.026065213621130567
1106	60-61	-0.05842666676291941
1106	62-63	-0.10652970371819492
1106	64-65	-0.06982615978132145
1106	66-67	-0.055010068769639986
1106	68-69	0.007791752214643566
1106	70-71	-0.047135222426057055
1106	72-73	-0.08007840424808421
1106	74-75	-0.11684870033870709
1106	76-77	-0.11374542802402487
1106	78-79	0.04407448637093836
1106	80-81	0.03147906344775464
1106	82-83	-0.04120907052084988
1106	84-85	0.017352913549444793
1106	86-87	0.048076427077425876
1106	88-89	0.015215245376431596
1106	90-91	0.03895478245887318
1106	92-93	-0.041039071419312734
1106	94-95	-0.037844412793084814
1106	96-97	0.06457705764234589
1106	98-99	0.1146947684771007
1106	100	0.1589649192075271
1107	1	0.11604469421110508
1107	2	0.08425694941620776
1107	3	0.05778158612756101
1107	4	0.02159498783319691
1107	5	0.022783579355248662
1107	6	0.03913740695656287
1107	7	0.06685786867350174
1107	8	-0.0028847694325477846
1107	9	0.025010612706140023
1107	10-11	0.0346807491571397
1107	12-13	0.02874236495880922
1107	14-15	0.041093151017115304
1107	16-17	0.07031838695057502
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2316	26-27	-0.008462233916809225
2316	28-29	0.010797681030389583
2316	30-31	-0.42104941341094815
2316	32-33	-0.17719188880129622
2316	34-35	-0.21138065593660826
2316	36-37	-0.49422450360165016
2316	38-39	-0.38174056083893504
2316	40-41	-0.2932067270253924
2316	42-43	-0.3934399394496566
2316	44-45	-0.2437013869102742
2316	46-47	-0.4187238326048828
2316	48-49	-0.38076516979329256
2316	50-51	-0.3321798398982878
2316	52-53	-0.35092402807453027
2316	54-55	-0.16582202742323204
2316	56-57	-0.8907379133720212
2316	58-59	-0.6989199833000654
2316	60-61	-0.40887003855880266
2316	62-63	-0.22562281963806896
2316	64-65	-0.10215284104092603
2316	66-67	-0.09907329075890203
2316	68-69	-0.16853524587163093
2316	70-71	-0.1756374919699084
2316	72-73	-0.08735975846119715
2316	74-75	-0.0428887891808003
2316	76-77	-0.08265637107795243
2316	78-79	-0.14116438154791666
2316	80-81	-0.12667831059297896
2316	82-83	-0.2674370188298596
2316	84-85	-0.08451254763204474
2316	86-87	-0.18935086557898018
2316	88-89	-0.22245200526469233
2316	90-91	-0.16382094062144859
2316	92-93	-0.23931479677576561
2316	94-95	-0.15873044088996835
2316	96-97	-0.38850084574783494
2316	98-99	-0.16379781145496253
2316	100	-0.3545510686890303
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	15.0
13	81.0
14	392.0
15	1235.0
16	3138.0
17	7285.0
18	13215.0
19	20724.0
20	29256.0
21	41792.0
22	54194.0
23	57716.0
24	55272.0
25	53796.0
26	57871.0
27	67268.0
28	81301.0
29	100575.0
30	123473.0
31	152642.0
32	193987.0
33	252031.0
34	371361.0
35	553029.0
36	964193.0
37	2014181.0
38	2613102.0
39	610912.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.102455830263445	19.393662998556227	13.933428167023868	23.57045300415646
2	15.916077517620316	20.36060724929987	40.21015821200701	23.5131570210728
3	18.826572099933163	25.623881789071557	30.3453940688038	25.20415204219148
4	12.921565649608965	17.115071502488195	38.355189654949136	31.608173192953704
5	13.351826044347423	37.58978463930796	34.52320558167331	14.535183734671314
6	30.508535036106306	36.817069671940565	18.387155599752912	14.287239692200215
7	26.8379683304498	32.84949429117414	22.339212942077292	17.97332443629877
8	25.8606966286976	35.126118484447325	20.348237825950548	18.664947060904534
9	26.437517630199757	16.09756735030636	20.69766615006355	36.76724886943033
10-11	24.969813284132798	26.076501581988172	28.24852062576931	20.705164508109725
12-13	25.51890729925216	23.755019516507666	28.219679277183733	22.50639390705644
14-15	23.064867336504282	25.235943405133714	26.309382004045585	25.389807254316416
16-17	22.27479068252868	27.88022365105096	27.039580881787245	22.80540478463312
18-19	21.872740906720022	27.238814602084364	29.14518691613816	21.743257575057452
20-21	22.96925422231881	26.486415977383178	28.882348876122276	21.661980924175737
22-23	22.7775345074783	26.288638159913525	28.622951906236832	22.31087542637134
24-25	22.327716143077478	26.206934995702376	28.993782009255	22.471566851965154
26-27	22.304729011142328	26.498382956019146	28.649754001286905	22.54713403155162
28-29	22.07375384701244	27.023555919922092	28.707510962491632	22.195179270573824
30-31	22.977857303854478	26.401955655506676	28.753652829353012	21.86653421128584
32-33	22.731595023219818	26.503312566724375	28.49999546683424	22.265096943221568
34-35	22.783392766881988	26.773113296352548	28.606300847062	21.83719308970347
36-37	22.666819496979866	26.674158648815194	28.44726697777879	22.211754876426156
38-39	22.62961952119182	26.752813582056373	28.513621900538418	22.103944996213393
40-41	22.798956596112642	26.244729171366632	28.678188271431953	22.27812596108877
42-43	22.561052711886674	26.53704935125607	28.277904042128444	22.623993894728812
44-45	22.37674764535297	26.872994013791324	28.442022896460873	22.308235444394835
46-47	22.36854103365831	26.531053870427584	27.98902240993069	23.11138268598341
48-49	22.547733030246103	26.654646634666758	28.063364085752756	22.73425624933438
50-51	22.641995910236172	26.78619299121992	27.89946984237727	22.67234125616664
52-53	22.44621596358107	27.080919661238905	27.63026706395653	22.842597311223496
54-55	22.29999207068172	26.799529939180893	27.904518999135192	22.995958991002198
56-57	22.66188826857669	26.851353091012285	27.504899078482907	22.981859561928115
58-59	22.294726815419203	27.002900635017564	27.888507588347757	22.813864961215476
60-61	22.66882986962448	27.04017432141447	27.698429792253993	22.592566016707057
62-63	22.70977726793681	26.8942149846153	27.54709946325425	22.84890828419364
64-65	22.296695932917977	26.732535549559827	27.638830646518954	23.33193787100324
66-67	22.401304010789133	26.649917004170483	27.5986165223008	23.350162462739586
68-69	22.095033414507657	26.743049411559717	27.769318930335473	23.392598243597146
70-71	22.231726819050625	26.500024634643516	27.746984963920106	23.521263582385753
72-73	22.23405090080865	26.77016698294074	27.532780237796274	23.463001878454335
74-75	22.388089048038783	26.794320171383617	27.38005904660415	23.43753173397345
76-77	22.163010151747894	26.67355203451435	27.365293095628225	23.79814471810953
78-79	21.99755688265196	26.638620420420562	27.571512865569918	23.79230983135756
80-81	21.978426650517576	26.856376080386298	27.407052872883376	23.75814439621275
82-83	22.346273448042112	26.637469117184114	27.64138284378787	23.374874590985904
84-85	22.496273439890906	26.7632561379269	27.17482448244771	23.565645939734477
86-87	21.864925780277787	27.002299550473907	27.408941592218543	23.72383307702976
88-89	21.949449843205475	26.441820616562467	27.513043351318217	24.09568618891384
90-91	22.187622804355254	26.328341379213725	27.53836087251045	23.94567494392057
92-93	22.10206778367186	26.477668647432278	27.443171209116247	23.97709235977962
94-95	21.683585681245177	26.50168150183161	27.531350458182025	24.28338235874119
96-97	21.612601154667498	26.46080248908441	27.548717354636725	24.377879001611362
98-99	21.453552863413822	26.47335973464632	27.393983674636115	24.679103727303744
100	21.53110701278611	26.45472060522829	27.310561532661055	24.70361084932455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1913.0
1	1340.0
2	1323.0
3	1779.0
4	1292.0
5	1034.5
6	1641.0
7	2991.0
8	4883.0
9	6151.0
10	5652.0
11	4910.0
12	5249.5
13	6017.0
14	6854.5
15	7707.5
16	8564.5
17	9512.5
18	10490.0
19	11463.0
20	12477.0
21	13636.0
22	15618.5
23	18421.0
24	21924.5
25	26739.5
26	33229.0
27	41332.5
28	51658.5
29	64659.5
30	80484.0
31	97035.5
32	116633.5
33	136638.0
34	154449.5
35	174113.0
36	195992.0
37	221361.5
38	238908.5
39	246372.5
40	250390.0
41	253889.5
42	262989.5
43	275901.0
44	289999.0
45	312644.5
46	333614.5
47	349038.5
48	362348.5
49	367160.0
50	366419.0
51	364599.0
52	355058.5
53	338343.0
54	320267.5
55	289290.5
56	256089.5
57	221719.0
58	186089.5
59	157929.0
60	127265.0
61	98705.0
62	77227.5
63	59582.5
64	43599.5
65	29804.5
66	20903.0
67	15515.5
68	11785.5
69	8882.5
70	6816.0
71	5206.0
72	4032.5
73	3111.0
74	2311.5
75	1598.0
76	1030.0
77	612.5
78	375.0
79	233.5
80	136.0
81	77.5
82	50.5
83	31.5
84	15.0
85	11.0
86	9.0
87	4.5
88	3.5
89	3.0
90	3.0
91	3.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35651289117321266
2	6.004080655758625E-4
3	0.002189723533276675
4	0.0013303159100014209
5	0.005015173253633675
6	0.0
7	8.240895017707918E-5
8	3.296358007083167E-4
9	0.0032374944712423963
10-11	7.652259659300209E-5
12-13	4.120447508853959E-5
14-15	2.943176792038542E-4
16-17	1.0006801092931043E-4
18-19	9.359302198682563E-4
20-21	1.8836331469046668E-4
22-23	1.5893154677008126E-4
24-25	0.0
26-27	0.0020543374008429025
28-29	0.011113435566737535
30-31	0.0034552895538532483
32-33	0.01485715644621056
34-35	0.011584343853463701
36-37	0.01346209064678429
38-39	0.013362022635854981
40-41	0.014727656667360865
42-43	0.006816397450361263
44-45	0.0013597476779218065
46-47	0.00653973883190964
48-49	0.013874135397669686
50-51	0.003932084194163492
52-53	0.005986421595006394
54-55	0.00504460502155406
56-57	2.7665861845162297E-4
58-59	5.709762976554771E-4
60-61	3.355221542923938E-4
62-63	8.358622089389459E-4
64-65	2.943176792038542E-4
66-67	0.0014303839209307313
68-69	0.0018600877325683587
70-71	0.0010301118772134898
72-73	0.003196289996153856
74-75	0.008976689215717554
76-77	0.004302924469960349
78-79	0.0018483150254002042
80-81	4.591355795580126E-4
82-83	2.0013602185862085E-4
84-85	0.0015245655782759647
86-87	0.004579583088411971
88-89	0.0017011561857982775
90-91	2.4722685053123753E-4
92-93	0.002366314140798988
94-95	0.003861447951154567
96-97	0.002071996461595133
98-99	0.002366314140798988
100	0.002801904306020692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8494223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.66210116930357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60765513279776	43.91587367368188
2	15.55059903682964	18.555628259572096
3	5.108754551462693	9.143970926955218
4	2.161932117960143	5.159416509716193
5	1.1453735257220785	3.4167695584136295
6	0.6631858159802377	2.374023554823605
7	0.40247007744131935	1.680854733454501
8	0.27474258507411087	1.3113375924966078
9	0.19456699424677243	1.0447448125462417
>10	0.8391875746520557	8.485124349912585
>50	0.03507980619839892	1.4255239154678288
>100	0.014869690826160737	1.6202941216716786
>500	9.924241776821873E-4	0.4054551089058374
>1k	4.961599731149422E-4	0.49846857993575255
>5k	2.3626664490997935E-5	0.07907948268540174
>10k+	7.087999347299381E-5	0.8834348197610513
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26798	0.31548500669219537	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23050	0.27136090022595355	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12824	0.15097319672440904	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.3545414336308335E-5	1.1772707168154167E-5
5	0.0	0.0	0.0	2.3545414336308335E-5	1.1772707168154167E-5
6	0.0	0.0	0.0	2.3545414336308335E-5	3.53181215044625E-5
7	0.0	0.0	0.0	3.53181215044625E-5	3.53181215044625E-5
8	0.0	0.0	0.0	3.53181215044625E-5	4.709082867261667E-5
9	0.0	0.0	0.0	4.709082867261667E-5	5.886353584077084E-5
10-11	0.0	0.0	0.0	5.297718225669375E-5	5.886353584077084E-5
12-13	0.0	0.0	0.0	6.474988942484791E-5	5.886353584077084E-5
14-15	0.0	0.0	0.0	7.652259659300209E-5	7.0636243008925E-5
16-17	0.0	0.0	0.0	8.240895017707918E-5	1.1184071809746459E-4
18-19	0.0	0.0	0.0	1.0006801092931043E-4	1.1772707168154168E-4
20-21	0.0	0.0	0.0	1.5893154677008126E-4	1.2949977884969585E-4
22-23	0.0	5.886353584077084E-6	0.0	4.061583973013188E-4	1.4127248601785E-4
24-25	0.0	1.1772707168154167E-5	0.0	9.06498451947871E-4	1.5304519318600418E-4
26-27	0.0	1.1772707168154167E-5	0.0	0.0019130649148250524	1.7070425393823544E-4
28-29	0.0	1.1772707168154167E-5	0.0	0.005132900325315218	1.7659060752231253E-4
30-31	0.0	1.1772707168154167E-5	0.0	0.016258108599220906	1.7659060752231253E-4
32-33	0.0	1.765906075223125E-5	0.0	0.04249947287703655	1.8836331469046668E-4
34-35	0.0	2.3545414336308335E-5	0.0	0.08448683299225838	1.8836331469046668E-4
36-37	0.0	2.3545414336308335E-5	0.0	0.15022562981923127	1.8836331469046668E-4
38-39	0.0	2.3545414336308335E-5	0.0	0.2435125614196849	1.8836331469046668E-4
40-41	0.0	2.3545414336308335E-5	0.0	0.34968472101568326	1.8836331469046668E-4
42-43	0.0	2.3545414336308335E-5	0.0	0.456575015748939	1.8836331469046668E-4
44-45	0.0	2.3545414336308335E-5	0.0	0.5847444786886334	1.8836331469046668E-4
46-47	0.0	2.3545414336308335E-5	0.0	0.7308673200597631	2.0013602185862085E-4
48-49	0.0	2.3545414336308335E-5	0.0	0.8898341849513487	2.0013602185862085E-4
50-51	0.0	2.3545414336308335E-5	0.0	1.0543283358583828	2.1190872902677503E-4
52-53	0.0	2.3545414336308335E-5	0.0	1.2194287811845768	2.1190872902677503E-4
54-55	0.0	2.3545414336308335E-5	0.0	1.410505704877303	2.177950826108521E-4
56-57	0.0	2.3545414336308335E-5	0.0	1.6275061297543048	2.4722685053123753E-4
58-59	0.0	2.3545414336308335E-5	0.0	1.872325461669655	2.589995576993917E-4
60-61	0.0	2.943176792038542E-5	0.0	2.118298518887484	2.589995576993917E-4
62-63	0.0	3.53181215044625E-5	0.0	2.3797880041529402	2.707722648675459E-4
64-65	0.0	3.53181215044625E-5	0.0	2.6385344486482163	2.707722648675459E-4
66-67	0.0	3.53181215044625E-5	0.0	2.934400238844683	2.7665861845162297E-4
68-69	0.0	3.53181215044625E-5	0.0	3.2579436635934798	2.825449720357E-4
70-71	0.0	3.53181215044625E-5	0.0	3.592129615622288	2.943176792038542E-4
72-73	0.0	3.53181215044625E-5	0.0	3.9245320025151207	3.237494471242396E-4
74-75	0.0	3.53181215044625E-5	0.0	4.271332410274606	3.4729486146054797E-4
76-77	0.0	5.886353584077084E-5	0.0	4.646393201591247	3.5318121504462506E-4
78-79	0.0	5.886353584077084E-5	0.0	5.061528288108283	3.590675686287021E-4
80-81	0.0	5.886353584077084E-5	0.0	5.4943224353775495	3.7672662938093335E-4
82-83	0.0	6.474988942484791E-5	0.0	5.934256729544304	4.002720437172417E-4
84-85	0.0	7.0636243008925E-5	0.0	6.384668733090714	4.002720437172417E-4
86-87	0.0	8.240895017707918E-5	0.0	6.854552794293251	4.120447508853959E-4
88	0.0	8.240895017707918E-5	0.0	7.2316208321820605	4.2381745805355006E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14325	0.0	54.428326	1
GTATCAA	25485	0.0	40.97696	1
ATCAACG	30705	0.0	33.244102	3
CAACGCA	30910	0.0	33.129272	5
TATCAAC	32245	0.0	32.035137	2
TCAACGC	32580	0.0	31.503796	4
AACGCAG	33025	0.0	31.333412	6
GTGGTAT	5585	0.0	29.136856	1
ACGCAGA	36630	0.0	27.929283	7
CGCAGAG	37495	0.0	27.209923	8
TGGTATC	5865	0.0	26.843435	2
GTACATG	29425	0.0	25.551626	1
TACATGG	29620	0.0	24.941858	2
GCAGAGT	40940	0.0	24.484373	9
ACATGGG	29735	0.0	24.165924	3
CATGGGG	23380	0.0	22.19146	4
GAGTACT	25510	0.0	22.051458	12-13
CAGAGTA	40335	0.0	21.455225	10-11
ATGGGGG	17065	0.0	21.232527	5
AGAGTAC	38060	0.0	20.632463	10-11
>>END_MODULE